Effect of camel milk protein hydrolysates against hyperglycemia, hyperlipidemia, and associated oxidative stress in streptozotocin (STZ)-induced diabetic rats

Kilari, Bhanu Priya; Mudgil, Priti; Azimullah, Sheikh; Bansal, Nidhi; Ojha, Shreesh; Maqsood, Sajid

doi:10.3168/jds.2020-19412

Cited by 43 publications

(31 citation statements)

References 39 publications

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“…Once accumulated in tissues, lipids can generate species that will lead to increased inflammation and oxidative stress inducing the progression of many diabetic complications ( Carlier et al, 2020 ). The hyperlipidemic effect of STZ was indicated by the increase of serum lipid levels in addition to the histology of liver tissue which displayed the presence of lipid accumulation in hepatocytes of diabetic rats compared with the control group ( Kilari et al, 2021 ). However, in harmony with our results, ( El-Kholy et al, 2018 ) reported marked amelioration in the lipid profile markers in BM-MSCs-treated diabetic rats; compared to the diabetic untreated rats.…”

Section: Discussionmentioning

confidence: 99%

AD-MSCs and BM-MSCs Ameliorating Effects on The Metabolic and Hepato-renal Abnormalities in Type 1 Diabetic Rats

El-Sawah

Rashwan

Althobaiti

et al. 2022

Saudi Journal of Biological Sciences

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Section: Discussionmentioning

confidence: 99%

AD-MSCs and BM-MSCs Ameliorating Effects on The Metabolic and Hepato-renal Abnormalities in Type 1 Diabetic Rats

El-Sawah

Rashwan

Althobaiti

et al. 2022

Saudi Journal of Biological Sciences

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“…Kilari et al [96] found that the histology of liver and pancreatic tissue displayed the absence of lipid accumulation in hepatocytes and preservation of β-cells in camel milk protein hydrolysate treated groups compared with the diabetic control group. Our results cleared that orall administration of fermented camel milk fortified with sage and mint leaves powder showed restoration of insulin secretion in diabetic rats and this means that the Langerhans islets β-cells restored their activity.…”

Section: Discussionmentioning

confidence: 99%

The biological effects of fermented camel milk fortified with sage (Salvia officinalis L.) and mint (Mentha piperita) leaves powder on alloxan-induced diabetic rats

Shahein

Elmeligy

El-Sayed

2022

Preprint

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Background: Diabetes mellitus (DM) is a chronic metabolic condition described by persistent hyperglycemia due to low secretion of insulin, insulin resistance, or a combination of both. Many studies suggested the potential anti-diabetic effect of camel milk and the important role of the bioactive components of mint and sage in decreasing the side effects of diabetes disease. This study was designed to assess the anti-diabetic potential of fermented camel milk fortified with sage or mint leaves powder (1 and 1.5%) in alloxan-induced diabetic rats. Methods: Forty-two adult normal male albino rats were taken for the study where one group was kept as the non-diabetic control group (6 rats) while others 36 rats were made diabetic by alloxan injection (150 mg/kg of body weight). Among diabetic rats, a control (+) group (6 rats) was kept and referred to as diabetic control whereas the other 5 groups (7rats each) of diabetic rats were fed on fermented camel milk (FCM) or fermented camel milk fortified with sage or mint leaves powder( 1 and 1.5%). Results: The oral administration of fermented camel milk fortified with sage or mint leaves powder caused a significant decreased in blood glucose level and lipid profile, and increased in insulin level compared to the control (+) and FCM groups, and the best results were observed with fermented camel milk fortified with 1.5% sage powder. The results also found that the fermented camel milk fortified with sage or mint leaves powder improved the liver and kidney functions of diabetic rats. Importantly, treatment of diabetic animals fermented camel milk fortified with sage or mint leaves powder resulted in significant amelioration of the histopathological changes of pancreatic, liver, and kidney observed in diabetic animals. Conclusion: Our study recommends the use of sage and mint leaves powder (at a ratio of 1.5%) with fermented camel milk to produce functional food products with anti-diabetic activity.

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“…Thus, the exact insulin levels in CM and its putative implication in the antidiabetic effects of CM is still a matter of debate and further studies are required to address this important aspect. Recent analytical studies on CM fractionation and processing suggest the existence of bioactive molecules (proteins/peptides) toward the key pathways involved in glucose homeostasis including insulin secretion by the pancreatic beta-cells as well as insulin action at the level of the major target tissues (liver, skeletal muscles, adipocytes) (32)(33)(34)(35)(36)(37)(38)(39). Moreover, other studies reported a link between diabetes and some CM components and proteins such as lactoferrin (40)(41)(42)(43)(44).…”

Section: Introductionmentioning

confidence: 99%

Camel Milk Targeting Insulin Receptor—Toward Understanding the Antidiabetic Effects of Camel Milk

et al. 2022

Self Cite

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Camel milk (CM) is known for its beneficial virtues in the human diet and health. This includes its antidiabetic properties demonstrated in many in vitro and in vivo studies. Nevertheless, the scientific rationale behind the molecular and cellular basis of such beneficial effects and the exact antidiabetic agent(s)/mechanism(s) are still elusive. In this review, we focused on the recent advances supporting the targeting of insulin receptor (IR) by CM components. Indeed, our recent work reported that CM proteins and derived peptides pharmacologically target IR in vitro leading to its activation and potentiation of insulin-mediated responses. The review describes the experimental approaches used to investigate the effects of CM on IR in vitro based on the fractionation of CM whey proteins to purify functional proteins and their hydrolysis by gastric proteases to generate bioactive peptides. In addition, we illustrated our cellular and molecular model consisting of studying the functional activity of CM fractions on IR and its downstream signaling pathways in the hepatocarcinoma (HepG2) and the human embryonic kidney (HEK293) cells using the bioluminescence resonance energy transfer (BRET), phosphorylation, and glucose uptake assays. Overall, our work demonstrated for the first time that CM lactoferrin and CM-derived bioactive peptides positively modulate IR and its related signaling pathways in HepG2 and HEK293 cells. As a conclusion, the pharmacological targeting of IR by CM sheds more light on the antidiabetic properties of CM by providing its molecular basis that may constitute a solid rationale for the development of new generation of antidiabetic tools from CM-derived proteins and peptides and the utilization of CM in the management of diabetes. The sequencing and the synthesis of the potent bioactive CM peptides may open promising perspectives for their application as antidiabetic agents.

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Effect of camel milk protein hydrolysates against hyperglycemia, hyperlipidemia, and associated oxidative stress in streptozotocin (STZ)-induced diabetic rats

Cited by 43 publications

References 39 publications

AD-MSCs and BM-MSCs Ameliorating Effects on The Metabolic and Hepato-renal Abnormalities in Type 1 Diabetic Rats

AD-MSCs and BM-MSCs Ameliorating Effects on The Metabolic and Hepato-renal Abnormalities in Type 1 Diabetic Rats

The biological effects of fermented camel milk fortified with sage (Salvia officinalis L.) and mint (Mentha piperita) leaves powder on alloxan-induced diabetic rats

Camel Milk Targeting Insulin Receptor—Toward Understanding the Antidiabetic Effects of Camel Milk

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