Effect of Camellia Oil on the Permeation of Flurbiprofen and Diclofenac Sodium through Rat and Pig Skin

Wang, Aiping; Seki, Toshinobu; Yuan, Dan; Saso, Yuko; Hosoya, Osamu; Chono, Sumio; Morimoto, Kazuhiro

doi:10.1248/bpb.27.1476

Cited by 9 publications

(8 citation statements)

References 10 publications

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“…In this study, we used human abdominal skins from 6 humans and selected three model drugs with different partition coefficients [11,16,18,26,32]. The log K ow values of NR, ISDN, and FP are -1.02 (low), 1.34 (intermediate), and 3.86 (high), respectively.…”

Section: Discussionmentioning

confidence: 99%

“…On the other hand, numerous animal models have been suggested as an alternative to human skin, including primate, porcine, mouse, rat, guinea pig, and snake skin [3,6,8,9,24,26,27,32,34]. Animal skins with a small variation in skin permeability may be much better suited than human skin for determining or estimating the skin permeabilities of drugs and for developing transdermal formulations [3,18,28].…”

Section: Introductionmentioning

confidence: 99%

“…The advantages of these animals are their small size and easy handling. Sprague-Dawley (SD) rats are broadly used for in vivo pharmacokinetic, pharmacological, and toxicological studies as parts of preclinical studies for drug development [14,15,19,32,35]. SD rats as well as hairless rats are often used for in vivo percutaneous absorption and in vitro skin permeation studies.…”

Section: Introductionmentioning

confidence: 99%

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Usefulness of Rat Skin as a Substitute for Human Skin in the in Vitro Skin Permeation Study

et al. 2011

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Sprague-Dawley (SD) rats are broadly used in preclinical studies for drug development, so a lot of information for the rats can be obtained especially from pharmacokinetic, pharmacological and toxicological studies. The purpose of this study was to clarify whether SD rat skin can be used to predict human skin permeability. In vitro permeation studies of the three model drugs, nicorandil, isosorbide dinitrate, and flurbiprofen, through human skin and SD rat skin were performed using Franz-type diffusion cells. The permeation rates of the three model drugs through human skin and SD rat skin were determined, and their variations were evaluated. The inter-individual variations in SD rat skin permeability of the three model drugs were much lower than that in human skin permeability, although the permeation rates of the three model drugs through the SD rat skin were about twice those through human skin. In addition, no difference in the skin permeability coefficients of the three model drugs was obtained between fresh SD rat skin and frozen SD rat skin. The markedly smaller variation in the permeability through SD rat skin compared with that through human skin indicated that in vitro permeation studies using SD rat skin would be especially useful for evaluating differences in the skin permeability of the three model drugs as well as for predicting human skin permeability.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Usefulness of Rat Skin as a Substitute for Human Skin in the in Vitro Skin Permeation Study

et al. 2011

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“…Numerous animal models, including primate, porcine, mouse, rat, guinea pig, and snake skin, have been suggested as alternatives to human skin [1,3,7,32,[60][61][62][63]. These animal skins with a small variation in skin permeability may be preferred compared with human skin for determining or estimating the skin permeabilities of drugs and for developing transdermal formulations [1,57,64].…”

Section: Intra-and Inter-individual Variationmentioning

confidence: 99%

Transdermal Permeation of Drugs in Various Animal Species

Todo

2017

Official Journal of the Japan Society of Drug Delivery System

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Excised human skin is utilized for in vitro permeation experiments to evaluate the safety and effect of topically-applied drugs by measuring its skin permeation and concentration. However, ethical considerations are the major problem for using human skin to evaluate percutaneous absorption. Moreover, large variations have been found among human skin specimens as a result of differences in age, race, and anatomical donor site. Animal skins are used to predict the in vivo human penetration/permeation of topically-applied chemicals. In the present review, skin characteristics, such as thickness of skin, lipid content, hair follicle density, and enzyme activity in each model are compared to human skin. In addition, intra-and inter-individual variation in animal models, permeation parameter correlation between animal models and human skin, and utilization of cultured human skin models are also descried. Pig, guinea pig, and hairless rat are generally selected for this purpose. Each animal model has advantages and weaknesses for utilization in in vitro skin permeation experiments. Understanding of skin permeation characteristics such as permeability coefficient (P), diffusivity (D), and partition coefficient (K) for each skin model would be necessary to obtain better correlations for animal models to human skin permeation.

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“…Recently, pig skin has been frequently used for skin permeation studies. 1,[17][18][19][20][21][22] It has been reported that the histological characteristics and permeability properties of pig skin closely resemble those of human skin. 6,7,[23][24][25][26][27][28] In addition, the Yucatan micropig (YMP) has been utilized for numerous research applications and has been suggested as an animal The purpose of this study was to evaluate the variations in the in vitro Yucatan micropig (YMP) skin permeabilities of drugs and to clarify whether YMP skin can be used to predict human skin permeability.…”

mentioning

confidence: 96%

Variation Assessment for in Vitro Permeabilities through Yucatan Micropig Skin

Takeuchi

Terasaka

Sakurai

et al. 2011

Biological & Pharmaceutical Bulletin

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Animal skins are frequently used as an alternative to human skin in percutaneous absorption studies, because human skin is not always available and the use of human tissues and organs creates ethical problems. In addition, a large variation has been found among human skin specimens as a result of differences in gender, age, race and anatomical donor site. [1][2][3][4][5][6][7][8][9][10] Numerous animal models, including primate, porcine, mouse, rat, guinea pig and snake, have been utilized for skin permeability studies. 1,3,4,[11][12][13] Animal skins with a small variation in skin permeability may be much better than human skin for determining or estimating the skin permeability of drugs and for developing transdermal formulations. 1,14,15) In our earlier study, 16) we investigated the in vitro permeation studies of the three model drugs, nicorandil (NR), isosorbide dinitrate (ISDN) and flurbiprofen (FP), through human abdominal skin (human skin) and Sprague-Dawley rat dorsal skin (SD rat skin) were performed using Franz-type diffusion cells. The three model drugs were selected because of their different log K ow (logarithm of octanol/water partition coefficient at 37°C) (Table 1). NR, ISDN and FP were used as hydrophilic, lipophilic and highly lipophilic drugs, respectively. The permeation rates of the three model drugs were determined, and their variations were evaluated. The inter-individual variations in human skin permeability for each model drug were larger than the intra-individual variations in human skin permeability. Although the interindividual variations in SD rat skin permeability for each model drug were much lower than those in human skin permeability, and the permeation rates of those drugs through the SD rat skin were approximately twice that through human skin.16) The markedly smaller variation in the permeability through SD rat skin compared with that through human skin indicated that the in vitro permeation studies using SD rat skin would be particularly useful for evaluating differences in the skin permeability of the three model drugs as well as for predicting human skin permeability. Recently, pig skin has been frequently used for skin permeation studies. 1,[17][18][19][20][21][22] It has been reported that the histological characteristics and permeability properties of pig skin closely resemble those of human skin. 6,7,[23][24][25][26][27][28] In addition, the Yucatan micropig (YMP) has been utilized for numerous research applications and has been suggested as an animal The purpose of this study was to evaluate the variations in the in vitro Yucatan micropig (YMP) skin permeabilities of drugs and to clarify whether YMP skin can be used to predict human skin permeability. In vitro permeation studies of the three model drugs, nicorandil, isosorbide dinitrate and flurbiprofen, through YMP skin were performed using Franz-type diffusion cells. The permeation rates of the three model drugs were determined, and their variations were evaluated. The inter-individual variations in YMP skin permeab...

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Effect of Camellia Oil on the Permeation of Flurbiprofen and Diclofenac Sodium through Rat and Pig Skin

Cited by 9 publications

References 10 publications

Usefulness of Rat Skin as a Substitute for Human Skin in the in Vitro Skin Permeation Study

Usefulness of Rat Skin as a Substitute for Human Skin in the in Vitro Skin Permeation Study

Transdermal Permeation of Drugs in Various Animal Species

Variation Assessment for in Vitro Permeabilities through Yucatan Micropig Skin

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