Effect of capsaicin treatment or inferior alveolar nerve resection on dentine formation and calcitonin gene-related peptide- and substance P-immunoreactive nerve fibres in rat molar pulp

Jacobsen, Ellen; Heyeraas, Karin J.

doi:10.1016/s0003-9969(96)00092-1

Cited by 27 publications

(31 citation statements)

References 31 publications

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“…Sensory nerve fibers play a possible role both in regulating the differentiation and proliferation of pulpal cells during the inflammation or healing processes following pulpal exposure or cavity preparation [1,3,9,10,13]. Sensory denervation showed a significant reduction in dentin formation at the pulp horn [8]. These findings support our opinion that the expression of CGRP plays a biological role in regulating the differentiation and function of odontoblasts during pulp wound healing and dentin bridge formation.…”

Section: Discussionsupporting

confidence: 74%

“…The immunohistochemical identification of nerve fibers has been used to evaluate sensory neuropeptides such as CGRP and substance P [5,8,10,13]. Although the pulpal nerve fibers that contain CGRP have recently been shown to play an important role in the pulp response to inflammation and wound healing [10,14], no studies have yet elucidated the relationship between the CGRP-IR nerve terminals and the differentiation of undifferentiated mesenchymal cells into fibroblast-like cells and odontoblasts during the healing process after a pulpotomy with the use of immunoelectron microscopy.…”

Section: Introductionmentioning

confidence: 99%

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Immunoelectron Microscopic Study of CGRP-Immunoreactive Nerve Terminals in Wound Healing and Dentin Bridge Formation after Pulpotomy in Rat Molar

Zhang¹,

Fukuyama²,

Zhang

et al. 2003

Acta Histochem. Cytochem

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The purpose of the present study was to investigate the relationship between the neuropeptide calcitonin gene-related peptide-immunoreactive (CGRP-IR) nerves and the differentiation of undifferentiated mesenchymal cells into fibroblast-like cells and odontoblasts during the healing process after a pulpotomy. The first maxillary molars from 56-day-old Wistar rats (n=60) were used. The rats were sacrificed to undergo an immunoelectron microscopic examination at 1, 3, 7, 14, and 28 days, postoperatively. In 1 to 3 postoperative days, numerous unmyelinated degenerated CGRP-IR nerve terminals were observed. In 7 postoperative days, regenerated terminals were found to contain numerous large granular vesicles, small clear vesicles, a few mitochondria and a labeled organelle. Certain terminals were found to be attached with the cell bodies of fibroblast-like cells and their processes at the fibrous matrix layer of the dentin bridge during healing process following a pulpotomy. In 14 to 28 postoperative days, CGRP-IR nerve terminals had come into contact with the differentiating odontoblasts at the odontoblast layer of the dentin bridge. These findings demonstrate strong evidence that CGRP may be related to both the proliferation and cytodifferentiation process as well as to the active function of the renewed odontoblasts in dentin bridge formation.

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Section: Discussionsupporting

confidence: 74%

Section: Introductionmentioning

confidence: 99%

Immunoelectron Microscopic Study of CGRP-Immunoreactive Nerve Terminals in Wound Healing and Dentin Bridge Formation after Pulpotomy in Rat Molar

Zhang¹,

Fukuyama²,

Zhang

et al. 2003

Acta Histochem. Cytochem

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“…There are immediate effects of inferior alveolar nerve transection on pulpal blood flow regulation Olgart et al, 1993), immune cell extravasation , and dentin formation (Jacobsen and Heyeraas, 1996;Heyeraas, 1989, 1990;Olgart et al, 1993), as summarized in more detailed reviews (Fristad,1997;Holland, 1996;Olgart, 1996). Similar denervation studies of sympathetic innervation found unusual neurochemistry, nerve sprouting, and immune cell entry in the affected pulps (Haug et al, 2001).…”

Section: Nerve Regeneration Tomentioning

confidence: 94%

Dental neuroplasticity, neuro‐pulpal interactions, and nerve regeneration

Byers

Suzuki

Maeda

2003

Microscopy Res & Technique

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This review covers current information about the ability of dental nerves to regenerate and the role of tooth pulp in recruitment of regenerating nerve fibers. In addition, the participation of dental nerves in pulpal injury responses and healing is discussed, especially concerning pulp regeneration and reinnervation after tooth replantation. The complex innervation of teeth is highly asymmetric and guided towards specific microenvironments along blood vessels or in the crown pulp and dentin. Pulpal products such as nerve growth factor are distributed in the same asymmetric gradients as the dentinal sensory innervation, suggesting regulation and recruitment of those nerve fibers by those specific factors. The nerve fibers have important effects on pulpal blood flow and inflammation, while their sprouting and cytochemical changes after tooth injury are in response to altered pulpal cytochemistry. Thus, their pattern and neuropeptide intensity are indicators of pulp status, while their local actions continually affect that status. When denervated teeth are injured, either by pulp exposure on the occlusal surface or by replantation, they have more pulpal necrosis than occurs for innervated teeth. However, small pulp exposures on the side of denervated crowns or larger lesions in germ-free animals can heal well, showing the value of postoperative protection from occlusal trauma or from infection. Current ideas about dental neuroplasticity, neuro-pulpal interactions, and nerve regeneration are related to the overall topics of tooth biomimetics and pulp/dentin regeneration.

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“…The immunohistochemical identification of nerve fibers has been used to evaluate dental innervation using neurofilament protein (Maeda et al 1986(Maeda et al , 1987Sato 1989) or sensory neuropeptides such as CGRP and substance P Swift and Byers 1992;Fristad et al 1994;Jacobsen and Heyeraas 1996). The rat molar has been used as a model for analyzing the dental responses of CGRP to exposure injury (Khayat et al 1988;Byers and Taylor 1993), cavity preparation (Taylor and Byers 1990;Swift and Byers 1992), tooth movement (Kvinnsland and Kvinnsland 1990), tooth replantation (Kvinnsland et al 1991), chronic dexamethasone treatment (Ferrari and Byres 1996), and inferior alveolar nerve resection (Jacobsen and Heyeraas 1996) in young adult rats.…”

Section: Introductionmentioning

confidence: 99%

“…The rat molar has been used as a model for analyzing the dental responses of CGRP to exposure injury (Khayat et al 1988;Byers and Taylor 1993), cavity preparation (Taylor and Byers 1990;Swift and Byers 1992), tooth movement (Kvinnsland and Kvinnsland 1990), tooth replantation (Kvinnsland et al 1991), chronic dexamethasone treatment (Ferrari and Byres 1996), and inferior alveolar nerve resection (Jacobsen and Heyeraas 1996) in young adult rats. Although these studies have shown CGRP-IR nerve fibers to play an important role in the pulp response to inflammation and wound healing, no studies have yet elucidated the relationship between CGRP innervation and dentin bridge formation following a pulpotomy in the rat molar.…”

Section: Introductionmentioning

confidence: 99%

CGRP immunohistochemistry in wound healing and dentin bridge formation following rat molar pulpotomy

Zhang

Fukuyama

1999

Histochemistry

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The aim of the present study was to investigate the neuropeptide CGRP in order to determine the effect on dentin bridge formation during the healing process after pulpotomy. First maxillary molars in 56-day-old Wistar rats (n=60) were used. The rats were killed for a neurohistopathological examination at 1, 3, 7, 14, and 28 days postoperatively. Neuronal changes in the residual pulp were studied using CGRP immunohistochemistry. By 1-3 days postoperatively, the CGRP-IR nerve fibers with abnormal beaded or knob-like structures were found to be more swollen than in the control and the leakage of a CGRP-IR-positive substance from the involved end of the nerve fibers was seen. At 7 days postoperatively, a vast number of newly sprouted CGRP-IR nerve fibers appeared in the residual pulp and some of them terminated in the differentiating odontoblast layer and the initial matrix layer of the dentin bridge. By 14-28 days, the nerve density had become progressively lower in the residual pulp. Regenerated axons also terminated in the odontoblast layer and the fibrous matrix layer of the calcified dentin bridge. These findings suggest that such sensory neuropeptides as CGRP may, therefore, play a role in dentin bridge formation in the rat molar.

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Effect of capsaicin treatment or inferior alveolar nerve resection on dentine formation and calcitonin gene-related peptide- and substance P-immunoreactive nerve fibres in rat molar pulp

Cited by 27 publications

References 31 publications

Immunoelectron Microscopic Study of CGRP-Immunoreactive Nerve Terminals in Wound Healing and Dentin Bridge Formation after Pulpotomy in Rat Molar

Immunoelectron Microscopic Study of CGRP-Immunoreactive Nerve Terminals in Wound Healing and Dentin Bridge Formation after Pulpotomy in Rat Molar

Dental neuroplasticity, neuro‐pulpal interactions, and nerve regeneration

CGRP immunohistochemistry in wound healing and dentin bridge formation following rat molar pulpotomy

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