Effect of caraway essential oil on the antioxidant and antimicrobial activity of chitosan film

Abstract

ABSTRACT:The aim of this study was to evaluate bioactivity of chitosan film with incorporated caraway essential oil by measuring antioxidant and antimicrobial activity. A Fourier transform infrared spectroscopy was used to determine the potential interaction of functional groups of chitosan film and incorporated caraway essential oil. New detected peaks and main shifts in the peaks of chitosan spectra are attributed mainly to presence of s-(+)-carvone and limonene, the main components of caraway essential oil. The antioxidant activity of chitosan film was analyzed by DPPH method. Chitosan film without incorporated caraway essential oil showed the lowest scavenging ability (29.95%, after 24 h). The addition of different concentrations of caraway essential oil into chitosan film significantly enhanced antioxidant activity of pure chitosan film, reaching the maximum of 95%. ASTM E 2149 -01 method was performed to evaluate the antimicrobial activity of chitosan films. The reduction of bacteria cell number in contact with examined films was tested on Gram-positive bacteria Staphylococcus aureus and Listeria monocytogenes and Gram-negative bacteria Escherichia coli and Salmonella Typhimurium. The most sensitive bacteria was Staphylococcus aureus and the most resistant bacteria was Salmonella Typhimurium for all tested films. These results suggested that incorporation of caraway essential oil into chitosan film significantly improved its antioxidant and antimicrobial activity. The film showed a great potential to be used as an active packaging material.

Development of chitosan edible film incorporated with Chrysanthemum morifolium essential oil [pdf]

“…According to Table 4, the readings of the scavenging effect increased from 4.97% to 18.63% as the concentration of essential oil increased from 1% to 4%. This trend is similar to results found by Hromiš et al (2015) that the antioxidant activity of chitosan film increased from 28% to 90% with the incorporation of 0% to 3% of Therefore, Table 4 showed the microbial inhibition of Chrysanthemum morifolium towards Staphylococcus aureus and Escherichia coli. The control film (0% Chrysanthemum morifolium chitosan film) and 1% Chrysanthemum morifolium chitosan film did not have inhibitory effects on the two bacteria.…”

“…The films were placed in the sample holder and fixed by a tiny probe. The spectra of the films were obtained with a resolution of 4 cm -1 as the average of 20 scans is within the range of 500 cm -1 to 4000 cm -1 against a background spectrum from an empty cell (Hromiš et al, 2015).…”

Chitosan/Thyme Oil Systems as Affected by Stabilizing Agent: Physical and Antimicrobial Properties

“…The FTIR spectrum of chitosan showed characteristic signals between 3500 and 3000 cm −1 related O-H and N-H bonds in the amino group. Due to the addition of TEO, this band was weakened, which suggests the fact that the most reactive functional amino and hydroxyl chitosan groups were weakened too, probably due to the interactions with thyme oil functional groups [28]. Bands located in the wavelength range from 2950 to 2800 cm −1 correspond to C-H stretching from the -CH 2 chitosan group.…”

Farklı Oranlarda Anason Tohumu Yağı Katkılı Kitosan Temelli Filmlerin Özelliklerinin Belirlenmesi

“…Due to its amine groups, chitosan is capable of interacting effectively with functional groups of various compounds and thus forming stable composite films [6,7]. In order to increase the structural, mechanical and antimicrobial properties of chitosan composite films, oil from plants such as garlic, bergamot, cumin, rosemary, cinnamon and thyme has been successfully incorporated into chitosan-based films [8][9][10][11][12]. In the current study, Pimpinella anisum seed oil was incorporated into chitosan-based films for the first time to enhance the structural properties of films.…”