Effect of Carbodiimide on Bonding Durability of Adhesive-cemented Fiber Posts in Root Canals

Abstract

This study was undertaken to investigate whether using a protein cross-linker, 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC), improves bonding stability of fiber posts to root dentin using three resin cements. Sixty human maxillary central incisor roots were randomly divided into six groups after endodontic treatment, according to the cements used with and without EDC pretreatment. In the etch-and-rinse group, 0.3 M EDC aqueous solution was applied on acid-etched root dentin prior to Excite DSC/Variolink II for post cementation. In the self-etch and self-adhesive groups, EDC was used on EDTA-conditioned root space prior to application of ED Primer II/Panavia F2.0 and Clearfil SA, respectively. After microslicing the root dentin, a push-out bond strength (BS) test was performed immediately or after one-year of water storage for each group. Data were analyzed using three-way analysis of variance and Tukey tests (α=0.05). A significant effect of cement type, time, EDC, and Time × Cement and Time × EDC interactions were observed (p≤0.001). EDC pretreatment did not affect immediate bonding of the three cements (p>0.05). Aging significantly reduced the BS in all the groups (p≤0.001), but EDC groups exhibited a higher BS compared with the respective control groups (p<0.001). Despite the significant effect of aging on decreasing the BS of fiber post to radicular dentin, EDC could diminish this effect for the three tested cements.

Effect of dimethyl sulfoxide on bond durability of fiber posts cemented with etch-and-rinse adhesives

“…This long-term water storage of root microslices prior to submitting to dislodging forces during the push-out test cannot closely mimic clinical aging conditions. However, this experimental set-up was previously designed to examine the role of MMP inhibitor agents in bond stability of fiber posts to root dentin 171840. Furthermore, direct exposure of micro-specimens was used to detect the effect of DMSO on bond stability in coronal dentin 16.…”

“…The other half of the samples were stored in 37℃ distilled water containing 0.4% sodium azide for 1 year before assessing the long-term bond strength 1718…”

Longevity of bond strength of resin cements to root dentine after radiation therapy

“…, Shafiei et al . ) when using conventional resin cements associated with total acid conditioning and self‐conditioning adhesive systems, as well as self‐adhesive resin cements (Shafiei et al . ), since the adhesive interface is exposed to degradation mechanisms of the exposed collagen by metalloproteinases and cysteine cathepsins over time (Bitter & Kielbassa , Shafiei et al .…”

“…), since the adhesive interface is exposed to degradation mechanisms of the exposed collagen by metalloproteinases and cysteine cathepsins over time (Bitter & Kielbassa , Shafiei et al . ). Therefore, the use of metalloproteinase inhibitors, such as chlorhexidine, and cross‐linking agents, such as carbodiimide (EDC), has been proposed as alternatives to improve the longevity of the adhesive interface (Shafiei et al .…”

Dual function of proanthocyanidins as both MMP inhibitor and crosslinker in dentin biomodification: A literature review

“…Crosslinking improves dentin mechanical properties and cause its resist against enzymatic degradations. Some agents such as glutaraldehyde (GD), carbodimide and proanthocyanidin (PA) are supposed to have both cross linking and MMP inhibitory effects [10][11][12][13][14] . Some drawbacks such as high cytotoxicity, mismatched mechanical properties and suboptimal long-term stability have been attributed to synthetic cross-linkers.…”