Effect of carbogen breathing on the physiological profile of human glioma xenografts

Sanden, Boudewijn P.J. van der; Heerschap, Arend; Hoofd, Louis; Simonetti, Arjan W.; Nicolay, Klaas; Toorn, Annette van der; Colier, Willy N.J.M.; Kogel, Albert J. van der

doi:10.1002/(sici)1522-2594(199909)42:3<490::aid-mrm11>3.0.co;2-h

Cited by 53 publications

(20 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It may be useful to combine R 2 * imaging with diffusion imaging, as the latter has been shown to be sensitive to necrosis in melanoma xenografts (43) and to tissue pO 2 in RIF-1 tumors where there was no necrosis (25). This spatial heterogeneity in response to carbogen has been observed in other studies using BOLD (14,21,22). One may be able to use such volumetric knowledge to better predict the overall response of the tumor to therapy, and/or the effectiveness of the carbogen in oxygenating the tumor.…”

Section: Macroscopic and Microscopic Heterogeneity Of The Bold Responmentioning

confidence: 75%

Changes in oxygenation of intracranial tumors with carbogen: A BOLD MRI and EPR oximetry study

Dunn

O’Hara

Wadghiri

et al. 2002

Magnetic Resonance Imaging

View full text Add to dashboard Cite

Purpose: To examine, using blood oxygen level dependent (BOLD) MRI and EPR oximetry, the changes in oxygenation of intracranial tumors induced by carbogen breathing. Materials and Methods:The 9L and CNS-1 intracranial rat tumor models were imaged at 7T, before and during carbogen breathing, using a multi-echo gradient-echo (GE) sequence to map R 2 *. On a different group of 9L tumors, tissue pO 2 was measured using EPR oximetry with lithium phthalocyanine as the oxygen-sensitive material. Results:The average decline in R 2 * with carbogen breathing was 13 Ϯ 1 s -1 in the CNS-1 tumors and 29 Ϯ 4 s -1 in the 9L tumor. The SI vs. TE decay curves indicate the presence of multiple components in the tumor. Tissue pO 2 in the two 9L tumors measured was 8.6 Ϯ 0.5 and 3.6 Ϯ 0.6 mmHg during air breathing, and rose to 20 Ϯ 7 and 16 Ϯ 4 mmHg (mean Ϯ SE) with carbogen breathing. Significant changes were observed by 10 minutes, but changes in pO 2 and R 2 * continued in some subjects over the entire 40 minutes. Conclusion:EPR results indicate that glial sarcomas may be radiobiologically hypoxic. Both EPR and BOLD data indicate that carbogen breathing increases brain tumor oxygenation. These data support the use of BOLD imaging to monitor changes in oxygenation in brain tumors.

show abstract

Section: Macroscopic and Microscopic Heterogeneity Of The Bold Responmentioning

confidence: 75%

Changes in oxygenation of intracranial tumors with carbogen: A BOLD MRI and EPR oximetry study

Dunn

O’Hara

Wadghiri

et al. 2002

Magnetic Resonance Imaging

View full text Add to dashboard Cite

show abstract

“…(i) After 1 min of carbogen breathing, the oxyhaemoglobin and deoxyhaemoglobin concentrations in human glioma xenografts were found to be close to their final steady-state values (van der Sanden et al, 1999). (ii) Upon carbogen breathing, an increase in tumour pO 2 has been reported for both animals and patients; a maximum was reached in patients after 1 -6 min (Guichard et al, 1994).…”

Section: Treatmentmentioning

confidence: 79%

Correlation between 5-fluorouracil metabolism and treatment response in two variants of C26 murine colon carcinoma

et al. 2003

View full text Add to dashboard Cite

Following an i.p. dose of 150 mg kg À1 5-fluorouracil (5-FU), drug uptake and metabolism over a 2-h period were studied by in vivo 19 F magnetic resonance spectroscopy (MRS) for the murine colon carcinoma lines C26-B (5-FU-insensitive; n ¼ 11) and C26-10 (5-FUsensitive; n ¼ 15) implanted s.c. in Balb/C mice. Time courses for tumour growth, intracellular levels of FdUMP, thymidylate synthase (TS) activity, and 5-FU in RNA were also determined, and the effects of a 9.5-min period of carbogen breathing, starting 1 min before drug administration, on MRS-detected 5-FU metabolism and tumour growth curves were examined. Both tumour variants generated MRS-detectable 5-FU nucleotides and showed similar initial growth inhibition after treatment. However, the growth rate of C26-B tumours returned to normal, while the sensitive C26-10 tumours, which produced larger fluoronucleotide pools, still showed moderate growth inhibition. Carbogen breathing did not significantly influence 5-FU uptake or fluoronucleotide production but did significantly enhance growth inhibition in C26-10 tumours. While both tumour variants exhibited incorporation of 5-FU into RNA and inhibition of TS via FdUMP, clearance of 5-FU from RNA and recovery of TS activity were greater for the insensitive C26-B line, indicating that these processes, in addition to 5-FU uptake and metabolism, may be important determinants of drug sensitivity and treatment response.

show abstract

“…Although many NIRS investigations have been conducted in the brain and breast in both laboratory and clinical settings over the past decade, there have been relatively few reports regarding solid tumors. 43,[96][97][98][99][100][101][102][103][104][105][106] Most studies to date have used reflectance mode. By contrast, we have favored transmission mode, so as to interrogate deep tumor regions, and we have presented preliminary studies in rat breast and prostate tumors with respect to various interventions.…”

Section: 9495mentioning

confidence: 99%

Therapeutic Vascular Targeting and Irradiation: Correlation of MRI Tissue Changes at Cellular and Molecular Levels to Optimizing Outcome

Zhao¹

2008

View full text Add to dashboard Cite

Public reporting burden for this collection of information is estimated to average 1 hour per response, including the time for reviewing instructions, searching existing data sources, gathering and maintaining the data needed, and completing and reviewing this collection of information. Send comments regarding this burden estimate or any other aspect of this collection of information, including suggestions for reducing this burden to Washington Headquarters Services, Directorate for Information Operations and Reports, 1215 Jefferson Davis Highway, Suite 1204, Arlington, VA 22202-4302, and Abstract (Maximum 200 Words) (abstract should contain no proprietary or confidential information)Vascular targeting agents (VTA) are new types of anticancer drugs that act on existing tumor vasculature, causing vascular disruption, which ultimately leads to extensive ischemic tumor cell death. Interesting findings showed that VTA killed cells predominantly in the more hypoxic tumor center, as a consequence of hemorrhagic necrosis after vascular collapse, whereas the better perfused peripheral rim was less affected. This apparently limits the effectiveness of such agents and rapid re-growth of tumor residues occurs. However, these findings suggest the possibility and promise of a combination of VTA with treatments specifically targeting the viable tumor rim. Radiation can certainly be expected to be most effective against the well-perfused and oxygenated cell populations at the peripheries of the tumors.One major goal of this project is to fully understand and precisely assess the dynamic changes in blood perfusion and oxygenation after VTA, so that we may predict response and optimize the therapy. I propose to use in vivo MRI to measure and assess physiological changes, e.g. tumor blood perfusion and dynamic tissue oxygenation, in the tumors before and after treatment. I believe non-invasive MRI approaches may provide a valuable prognostic tool to predict the response of specific breast tumors to VTA. Introduction:Targeting tumor vasculature promises effective cancer therapy (1, 2). It avoids issues of drug delivery and is potentiated by massive downstream effects where one blood vessel may supply the nutrients for thousands of tumor cells. Thus, disrupting the vascular supply should generate magnified tumor cell kill. Thus, inhibiting the growth of new blood vessels, i.e., antiangiogenesis, should prevent growth and metastasis of the primary tumor (1, 2). In addition to the focus on the antiangiogenic approaches, vascular targeting, directly attacking the existing neovasculature, is an alternative strategy against the tumor blood vessel network. Tubulin binding agents, e.g., combretastatin A-4-phosphate (CA4P) represent one kind of vascular targeting agent (VTA) (3-5). Promising preclinical studies have shown that such agents selectively cause tumor vascular shutdown and subsequently trigger a cascade of tumor cell death in experimental tumors (4-6). However, survived tissues in a thin viable rim of tumor usually re-grow ...

show abstract

Effect of carbogen breathing on the physiological profile of human glioma xenografts

Cited by 53 publications

References 30 publications

Changes in oxygenation of intracranial tumors with carbogen: A BOLD MRI and EPR oximetry study

Changes in oxygenation of intracranial tumors with carbogen: A BOLD MRI and EPR oximetry study

Correlation between 5-fluorouracil metabolism and treatment response in two variants of C26 murine colon carcinoma

Therapeutic Vascular Targeting and Irradiation: Correlation of MRI Tissue Changes at Cellular and Molecular Levels to Optimizing Outcome

Contact Info

Product

Resources

About