Effect of Carbohydrates on the Ability of Bull Sperm to Bind to Bovine Oviduct Epithelial Cells

Kon, Yoshihiro; Iwata, Hisataka; Shiono, H.; Matsubara, Kazuei; Kurita, A; Sakaguchi, Yosuke; Kuwayama, Takehito; Monji, Yasunori

doi:10.1111/j.1439-0531.2007.01013.x

Cited by 6 publications

(4 citation statements)

References 33 publications

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“…Furthermore, sperm-somatic cell interaction depends on the carbohydrate chains [23]. In addition, 5 mM GlcNAc increases sperm binding to oviduct cells in bovine [24]. Therefore, the contradictory results might be due to differences in oocyte state (COCs vs. denuded oocytes) and/or GlcNAc concentrations (50 vs. 5 mM).…”

Section: Discussionmentioning

confidence: 81%

Effect of N-Acetyl-D-Glucosamine on Bovine Sperm-Oocyte Interactions

Sakaguchi

Iwata

Kuwayama

et al. 2009

Journal of Reproduction and Development

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Abstract. N-Acetyl-D-glucosamine (GlcNAc) is a major component of glycosaminoglycan, which is involved in spermoocyte interactions. We examined the effect of adding GlcNAc and other monosaccharides, D-mannose and D-fucose, to the in vitro fertilization (IVF) medium on bovine sperm-oocyte interactions. In medium in which sperm and a zona pellucida (ZP) were co-incubated with monosaccharides for 5 min, addition of GlcNAc (5 or 25 mM) significantly reduced the number of sperm that attached to the ZP. Pretreatment of gametes with GlcNAc (5 mM) prior to coincubation also suppressed sperm-ZP attachment. Addition of GlcNAc (5 or 25 mM) to the medium in which sperm and a ZP were co-incubated for 5 h, however, significantly increased the number of sperm binding to and penetrating the ZP in a concentration-related manner. The other monosaccharides, D-fucose and D-mannose, did not have this effect. Supplementation of the sperm-oocyte co-incubation medium with 5 mM GlcNAc also enhanced the rate of polyspermic fertilization. When the ZPs were removed from the oocytes, GlcNAc did not affect the fertilization rate. Furthermore, incubation of sperm with 5 mM GlcNAc induced sperm membrane destabilization and an acrosome reaction, as evidenced by the hypo-osmotic swelling test and fluorescein isothiocyanate-labeled peanut agglutinin/ propidium iodide (FITC-PNA/PI) staining. Finally, GlcNAc suppressed ZP hardening following fertilization, as determined by measuring the time required for pronase to dissolve the ZP. In conclusion, supplementation of IVF medium with GlcNAc has various effects on sperm-oocyte interactions including suppression of initial attachment, induction of sperm membrane destabilization and acrosome reaction, increase in the number of sperm secondarily bound to and penetrating the ZP, suppression of ZP hardening following sperm-oocyte co-incubation and increase in the rate of polyspermic fertilization. Key words: Bovine, Fertilization, Monosaccharides, Sperm, Zona pellucida (J. Reprod. Dev. 55: [676][677][678][679][680][681][682][683][684] 2009) ertilization of oocytes comprises several interactions between the sperm and oocyte. Contact between the sperm and oocyte first occurs at the outer surface of the surrounding extracellular matrix of the egg, the zona pellucida (ZP). Binding of the lectinlike protein on the sperm head to distinct oligosaccharides of ZP glycoproteins leads the capacitated sperm to recognize the oocyte. Upon anchoring of the sperm to the oocyte through the ZP carbohydrates, a signaling cascade induces the acrosome reaction of the sperm, allowing the sperm to pass through the ZP. The acrosomereacted, penetrated sperm fuses with the egg plasma membrane, which enables the egg to complete meiosis, and initiates mechanisms to prevent polyspermic fertilization by the exocytosis of cortical granules (CG) [1]. Oligosaccharides on the ZP include some monosaccharides, such as N-acetyl-D-glucosamine (GlcNAc), D-mannose and D-fucose [2], and these monosaccharides are involved in many sperm-oocyte in...

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Section: Discussionmentioning

confidence: 81%

Effect of N-Acetyl-D-Glucosamine on Bovine Sperm-Oocyte Interactions

Sakaguchi

Iwata

Kuwayama

et al. 2009

Journal of Reproduction and Development

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“…Spermatozoa incubated with OEC cultures showed higher penetration and monospermy rates when short time periods were used (Hunter 1981). Moreover, the fertilization and cleavage rates of spermatozoa unbound to oviductal cells culture were lower than those for spermatozoa attached to the OEC (Kon et al 2009). These results indicate that the ability of spermatozoa to bind to oviductal cells is related to their fertilizing ability.…”

Section: Discussionmentioning

confidence: 96%

Boar sperm tyrosine phosphorylation patterns in the presence of oviductal epithelial cells: in vitro, ex vivo, and in vivo models

Luño¹,

López‐Úbeda²,

García–Vázquez³

et al. 2013

Reproduction

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Spermatozoa transport through the oviduct is a controlled process that regulates sperm capacitation. A crucial event involved in capacitation is protein tyrosine phosphorylation (TP). This study was undertaken to determine whether similarities exist in protein TP distribution between spermatozoa bound or unbound to oviductal epithelial cells (OEC) in three different conditions: i) in vitro, spermatozoa coincubated with OEC cultures; ii) ex vivo, spermatozoa deposited in porcine oviductal explants from slaughtered animals; iii) in vivo, in which sows were inseminated and the oviduct was recovered. The localization of phosphotyrosine protein was determined using indirect immunofluorescence. The distribution of protein TP was significantly (P!0.05) different between bound and unbound cell populations in all experiments. In sows inseminated close to ovulation, spermatozoa were found mainly in the utero-tubal junction, where spermatozoa exhibited higher proportion of flagellum phosphorylation. Spermatozoa not bound to OEC exhibited high levels of protein phosphorylation (phosphorylated equatorial subsegment and acrosome and/or phosphorylated flagellum) in the ex vivo and in vivo experiments (P!0.05). However, unbound spermatozoa coincubated with OEC in in vitro conditions tended to show intermediate levels of TP (equatorial subsegment with or without phosphorylated flagellum). In spermatozoa bound to OEC, protein TP was located in the equatorial subsegment or presented no phosphorylation (P!0.05). Although sperm capacitation conditions in vivo were not reproducible in vitro in our experimental conditions, sperm and OEC binding seemed to be a mechanism for selecting spermatozoa with a low level of TP in in vivo, ex vivo, and in vitro experiments.

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“…Regarding the reservoir assembly, structural studies have pointed at the interaction between the rostral portion of the acrosome of the bull sperm and the OEC microvilli [3,36,54]. Several studies have suggested the participation of species-specific carbohydrate recognition in spermoviduct interaction [55][56][57], and specific proteins have been proposed to play a role in the formation of the oviduct sperm reservoir; among them are seminal plasma protein PCD-109 (BSP-A1/A2) [58,59], BSP-A3, and BSP-30-kDarelated proteins [60] in bovine spermatozoa. These proteins appear to be lost from the plasma membrane overlying the head during sperm capacitation, and this event is associated to a decrease in OEC-sperm binding [59,61,62].…”

Section: Discussionmentioning

confidence: 99%