Effect of carbon and nitrogen limitation on lignin peroxidase and manganese peroxidaseproduction by Phanerochaete flavido‐alba

Abstract

The ligninolytic enzymes lignin peroxidase (LiP) and manganese dependent peroxidase (MnP), were detected in extracellular fluids of Phanerochaete flavido-alba FPL 106507 cultures under carbon or nitrogen limitation. MnP activities were found to be higher than LiP activities under all growth conditions tested. Higher titres of both peroxidases were obtained under carbon limitation in excess nitrogen. Isoelectric points (pIs) observed after FPLC and IEF of concentrated extracellular fluids revealed more acidic pIs for LiP enzymes obtained in nitrogen-limited cultures than those in carbon-limited cultures. However, the change in the limiting growth factor does not significantly affect MnP pIs.

Biosurfactants are useful tools for the bioremediation of contaminated soil: a review

“…White-rot fungi secrete one or more of the three enzymes that are essential for the degradation of lignin: laccase (Lac), lignin peroxidase (LiP), and Mn dependant peroxidase (MnP) (Reddy, 1995;Pointing, 2001;Eichlerová et al, 2005). These enzymes are secreted during secondary metabolism, as a result of nutrient depletion of carbon or sulphur, or especially, manganese and nitrogen (Hamman et al, 1997;Leung and Pointing, 2002). Other factors are also implicated, such as initial pH of the medium and incubation temperature , the level of aeration and agitation during incubation, the availability of mediator compounds and metals (Leung and Pointing, 2002;Mouso et al, 2003;Cordi et al, 2007), or the presence and concentration of surfactants (Rodríguez Couto et al, 2000;Ürek and Pazarlioğlu, 2005;Wang et al, 2008).…”

Peroxidase production from hairy root cultures of red beet (Beta vulgaris)

“…This was in contrast to the earlier reports where NO 3 -had very little effect on secretion of POD (Uozumi et al 1992). However, certain white rot fungi such as Phanerochaete flavido-alba has also been observed to respond positively to carbon limitation and excess of nitrogen leading to the leaching of lignin POD (Hamman et al 1997).…”

The effect of manganese on the production of Phanerochaete flavido-alba ligninolytic peroxidases in nitrogen limited cultures

“…In order to increase the yield in enzymatic activities of the cultures, these experiments were performed with 1 l Erlenmeyer £asks (70 ml culture media each), and P. £avido-alba ultra¢ltration concentrated extracellular £uids were obtained as previously [12].…”

“…£avido-alba extracellular laccase was partially characterized from high nitrogen, cultures not sup-plemented with Mn(II) [8]. In nitrogen limited cultures P. £avido-alba haemproteins (M r about 45 kDa) [11] showed pIs lower than 3.55 (LiPs) [12] and a whole range of pIs (lower than 3.55 to 5.85) for MnPs [11].…”