Effect of carbon source on the accumulation of cytochrome <i>P</i>-450 in the yeast <i>Saccharomyces cerevisiae</i>

Kärenlampi, Sirpa; Marín, E.; Hänninen, Osmo

doi:10.1042/bj1940407

Cited by 26 publications

(9 citation statements)

References 21 publications

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“…Unlike iso-1-cytochrome c and iso-2-cytochrome c, the apoproteins of cytochrome b (31), cytochrome c peroxidase, and cytochrome c1 (24) are not diminished in anaerobically grown cells. Also unlike iso-1-cytochrome c and iso-2-cytochrome c, cytochrome P-450 is present in glucose-grown cells but absent in derepressed respiring cells (32). Thus, various heme proteins respond differently to the induction by heme, the induction by oxygen, and the repression by glucose.…”

Section: Resultsmentioning

confidence: 91%

Differential regulation of the duplicated isocytochrome c genes in yeast.

Laz¹,

Pietras²,

Sherman³

1984

Proc. Natl. Acad. Sci. U.S.A.

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The two unlinked genes CYCI and CYC7 encode iso-l-cytochrome c and iso-2-cytochrome c, respectively, in the yeast Saccharomyces cerevisiae. An examination of the steady-state level of CYCI and CYC7 mRNAs in normal and mutant strains grown under different conditions, along with previous results of apoprotein levels, demonstrate that CYCI and CYC7 have similar and different modes of regulation. Both CYCI and CYC7 mRNAs are diminished after anaerobic growth. In contrast, CYCI mRNA but not CYC7 mRNA is decreased by heme deficiency in hem) mutants. Although both CYCI and CYC7 mRNAs are substantially lowered after growth in glucose medium, there is a difference in the kinetics of glucose derepression. CYCI mRNA levels rise in the early logarithmic phase of growth before complete exhaustion of glucose, whereas CYC7 mRNA levels rise in the late logarithmic phase when the level of CYCI mRNA has plateaued. For a brief period before cessation of growth, the level of CYC7 mRNA attains a level corresponding to the high derepressed level of CYCJ mRNA. The high amount of CYC7 mRNA is surprising because iso-2-cytochrome c constitutes only 5% of the total cytochrome c complement in derepressed cells. We suggest that iso-2-cytochrome c has the potential to comprise a major proportion of cytochrome c under certain physiologic conditions that have not been experimentally defined. The cyc3 mutant, which lacks the ability to attach heme groups to apocytochromes c, contains both CYCI and CYC7 mRNAs in normal amounts. Yet, cyc3 mutants contain only apoiso-2-cytochrome c and not apoiso-1-cytochrome c. The lack of accumulation of apoiso-1-cytochrome c in cyc3 mutants, which contain CYCI mRNA, suggests that apoiso-1-cytochrome c is extensively regulated by a post-transcriptional process.The total complement of cytochrome c in derepressed cells of the yeast Saccharomyces cerevisiae is typically composed of 95% iso-1-cytochrome c and 5% iso-2-cytochrome c (1).Both iso-1-cytochrome c and iso-2-cytochrome c carry out identical or similar functions in mitochondrial electron transport (2). Iso-1-cytochrome c and iso-2-cytochrome c are encoded by the unlinked nuclear genes CYCJ and CYC7 (3-6), respectively, that are -=80% homologous in their DNA sequence (7) and that appear to be evolutionarily related (8). The biosynthesis of the two isocytochromes c, similar to the biosynthesis of other heme proteins, is decreased by growth in glucose media (catabolite repression) and by growth with anaerobic conditions (reviewed in ref.

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Section: Resultsmentioning

confidence: 91%

Differential regulation of the duplicated isocytochrome c genes in yeast.

Laz¹,

Pietras²,

Sherman³

1984

Proc. Natl. Acad. Sci. U.S.A.

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“…Accordingly, cytochrome P450 accumulates both in the presence of fermentable carbon sources, especially glucose, and when the oxidative metabolism is repressed. 93,193 Translational and post-translational regulation by glucose have also been proposed. 187 Among the three CYP genes encoding cytochrome P450 in S. cerevisiae [i.e.…”

Section: Regulation Of Sterol Synthesis By Oxygen and Glucosementioning

confidence: 99%

Role of the non‐respiratory pathways in the utilization of molecular oxygen by Saccharomyces cerevisiae

Rosenfeld

Beauvoit

2003

Yeast

133

110

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Saccharomyces cerevisiae is a facultative anaerobe devoid of mitochondrial alternative oxidase. In this yeast, the structure and biogenesis of the respiratory chain, on the one hand, and the functional interactions of oxidative phosphorylation with the cellular energetic metabolism, on the other, are well documented. However, to our knowledge, the molecular aspects and the physiological roles of the non-respiratory pathways that utilize molecular oxygen have not yet been reviewed. In this paper, we review the various non-respiratory pathways in a global context of utilization of molecular oxygen in S. cerevisiae. The roles of these pathways are examined as a function of environmental conditions, using either physiological, biochemical or molecular data. Special attention is paid to the characterization of the so-called 'cyanide-resistant respiration' that is induced by respiratory deficiency, catabolic repression and oxygen limitation during growth. Finally, several aspects of oxygen sensing are discussed.

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“…This cyto-chrome is formed adaptively when the yeast is grown aerobically and supplied n-alkanes as a sole carbon source (64,90,91). In addition to these conditions, growth phase of the yeast and glucose concentration in the growth medium are known to affect the cytochrome P450 content in the yeast (54,64,83). The role of cyclic AMP on induction of cytochrome P450 has been described (104).…”

Section: Effects Of Growth Conditions On Cytochrome Compositionmentioning

confidence: 99%