Effect of Carbon Sources and Sucrose Concentrations on Shoot Organogenesis of Aloe saponaria

Abstract

In the present study, the effect of various carbon sources and sucrose concentrations on in vitro organogenesis of Aloe saponaria was investigated and a rapid micropropagation protocol was developed from in vitro-derived meristem explants. Meristem explants were cultured in initial shoot regeneration media with five different carbon sources (fructose, glucose, lactose, maltose, and sucrose), and sucrose as the best carbon sources for shoot regeneration and shoot elongation was investigated at five different concentrations (

Influence of Different Types of Carbon Sources on Glucosinolate and Phenolic Compounds in Radish Sprouts

“…Similar results were reported in Echinacea angustifolia, in which the plants supplemented with sucrose showed the highest shoot length [43]. In addition, the highest shoot regeneration of an Aloe saponaria explant was obtained in the sucrose treatment, followed by glucose, and fructose treatments [44]. In Pharbitis nil, glucose, fructose, and maltose showed very low levels of shoot regeneration and elongation [45].…”

An efficient protocol for in vitro direct shoot organogenesis of Sesamum indicum L. using cotyledon as explant

“…Previously, Seo et al (2007) reported that pre-culture of cotyledon explants for 2 weeks on 9% sucrose and subsequent sub-culture to medium with 3% sucrose significantly enhanced adventitious shoot formation in sesame. In a recent study, Kim et al (2016) reported that 4% sucrose concentration was necessary for shoot organogenesis in Aloe saponaria. For further investigation of the assumption of ABA-mediated sucrose uptake, cotyledons explants were cultured in varying concentrations of sucrose (3 to 9%) supplemented in the AG 3 medium independently for 7 days and explants from every treatment were sub-cultured in AG 3 medium that was supplemented with 3% sucrose only.…”