Effect of catechin on the activity and gene expression of superoxide dismutase in cultured rat brain astrocytes

Chan, Paul; Cheng, Juei Tang; Tsai, Jo Ting; Lien, Gi Shih; Chen, Fu Chean; Kao, Pai Feng; Liu, Ju Chi; Chen, Yi Jen; Hsieh, M. H.

doi:10.1016/s0304-3940(02)00552-9

Cited by 38 publications

(27 citation statements)

References 18 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Recent evidence suggests that ROS, such as superoxide anions and hydrogen peroxide, may play a role as intracellular second messengers regulating the transcription of SOD [23]. In the previous reports from our laboratory, we found that SOD gene expression and activity also can be regulated by other antioxidants, such as catechin [21] and trilinolein [24]. These results implicated that the antioxidant effect of ascorbate in the present study may be one of the major mechanisms related to SOD changes.…”

Section: Discussionsupporting

confidence: 82%

“…Vitamin C is much more effective than vitamin E and ß-carotene in preventing low-density lipoprotein oxidation in vitro [16]. Several previous studies performed by the same group [17][18][19][20][21] have shown that naturally existing antioxidants such as trilinolein isolated from the natural herb Panax pseudoginseng, catechin isolated from tea, and vitamins E and C all should be beneficial to the SOD system [17][18][19][20][21]. The present data revealed that short-term (2 days) and long-term (7 days) incubation with vitamin C resulted in a decrease of SOD activity and mRNA.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Downregulation of Superoxide Dismutase Activity and Gene Expression in Cultured Rat Brain Astrocytes after Incubation with Vitamin C

Kao¹,

Lee²,

Liu³

et al. 2003

Pharmacology

Self Cite

View full text Add to dashboard Cite

Reactive oxygen species have been linked with neuropathological changes in the central nervous system. Epidemiological studies supported the beneficial effect of supplementation of antioxidants. Superoxide dismutase (SOD) is an endogenous enzyme which can scavenge reactive oxygen species. This study investigated the effect of supplementation with ascorbic acid (vitamin C) on the changes of SOD in cultured neurological cells. Rat brain astrocytes (RBA-1 cells) were incubated with vitamin C and divided into four groups: a control group (without vitamin C) and three treatment groups with vitamin C at 40, 80, and 160 µmol/l. After short-term (2 days) and long-term (7 days) incubation, SOD activity, SOD mRNA level by Northern blotting, and SOD protein amounts by Western blotting were measured. After 2 days of incubation, vitamin C resulted in a decrease in the activity of SOD in a concentration-dependent manner (Mn-SOD from 14.8 ± 1.2 to 13.2 ± 0.5 U/mg protein and Cu/Zn-SOD from 64.8 ± 1.2 to 51.7 ± 0.9 U/mg protein; p < 0.05), and vitamin C also attenuated the Cu/Zn-SOD mRNA level from 100 to 86.3 ± 6.7%; p < 0.01), whereas the protein amounts of these two SODs remained unchanged. After 7 days of incubation with vitamin C, the SOD activity of RBA-1 cells decreased significantly (Mn-SOD from 14.9 ± 0.3 to 11.8 ± 0.3 U/mg protein and Cu/Zn SOD from 61.8 ± 1.8 to 54.6 ± 0.9 U/mg protein; p < 0.01), and the mRNA level was also attenuated (Mn-SOD from 100 to 86.8 ± 8.7% and Cu/Zn-SOD from 100 to 84.7 ± 4.8%; p < 0.01). These results suggest that 2 and 7 days of incubation with relatively high concentrations of vitamin C may downregulate activity and gene expression of SOD in cultured RBA-1 cells.

show abstract

Section: Discussionsupporting

confidence: 82%

Section: Discussionmentioning

confidence: 99%

Downregulation of Superoxide Dismutase Activity and Gene Expression in Cultured Rat Brain Astrocytes after Incubation with Vitamin C

Kao¹,

Lee²,

Liu³

et al. 2003

Pharmacology

Self Cite

View full text Add to dashboard Cite

show abstract

“…These areas were quantified by the image processing program JAVA (Jandel Scientific Software) and the total infarct volume was calculated by integrating the infarcted area of all 20 sections (area of infarct in mm 2 x section thickness). To compensate for swelling, the following formula was applied: corrected infarct size = infarct size x contralateral hemisphere size / ipsilateral hemisphere size, according to Chan et al (2002). Measurements were performed someone blinded to the treatment of the processed animal tissue.…”

Section: Morphometric Determination Of Infarct Volumementioning

confidence: 99%

Some aspects of thein vivo neuroprotective capacity of flavonoids: Bioavailability and structure-activity relationship

et al. 2004

View full text Add to dashboard Cite

On the basis of previous work showing that flavonoids structurally related to quercetin are neuroprotective for cells in culture, this work was directed towards determining if several flavonoids (quercetin, fisetin and catechin) could acutely and by an intraperitoneal (IP) route reach significant cerebral concentrations and either prevent or facilitate recovery from a brain lesion induced by focal ischemia in rats. Aqueous and liposomal preparations of quercetin, fisetin and catechin were administered IP in a single dose and assessed in the brain by HPLC at 30 min, 1 h, 2 h and 4 h. Ischemic damage from focal middle cerebral artery occlusion was assessed spectrophotometrically with 2,3,5,-triphenylltetrazolium chloride (TTC). Infarct volume was assessed by an image analysis system following perfusion with TTC. The status of the cerebral tissue was evaluated by hematoxylin-eosin. Flavonoids administered in aqueous preparations were undetected in the brain. Cerebral concentrations of catechin (10.5 ng/g), fisetin (8.23 ng/g) and quercetin (509 ng/g) were detected in the brain only after IP injection of the liposomal preparations. Spectrophotometric analysis of brain tissue with the TTC-technique showed that liposomal quercetin reduced ischemic damage and infarct volume after permanent occlusion of the middle cerebral artery (ischemic: 41.3 mm3 vs liposomal quercetin: 17 mm3). In liposomal quercetin-treated animals there was also recovery of the cytoarchitecture in ischemic areas of striatum and cortex. Although a liposomal preparation of fisetin had similar effects, catechin failed to protect brain tissue. In conclusion, early administration of liposomal preparations of quercetin and structurally related flavonoids are beneficial and neuroprotective in experimental focal ischemia.

show abstract

“…While some research, in vivo (rats) and in vitro, has indicated that green tea or catechins may protect the nervous system, by suppression of lipid peroxidation [18,19], there are no reports on the effects of intake of catechin alone, or in combination with DHA, on animal behavior. Furthermore, the relationship between a combined intake of these compounds and brain fatty acid composition is not clear.…”

Section: Introductionmentioning

confidence: 99%

Effect of Dietary Docosahexaenoic Acid and Catechins on Maze Behavior in Mice

Shirai

Suzuki²

2004

Ann Nutr Metab

View full text Add to dashboard Cite

Background/Aims: Docosahexaenoic acid (DHA; 22:6 n–3) and catechins are food components that play an important role in maintaining human health. However, the effect of a simultaneous intake of DHA and catechins on brain function is unknown. The purpose of this study was to investigate the effect of DHA and catechins on maze behavior in mice. Method: Adult (5 months old) and old (15 months old) male mice were fed 5% lard diets containing 0 or 1.5% DHA ethyl ester (DHA-EE), either with or without 0.5% catechins, for 3.5 months. Maze behavior was assessed 3 months after the start of the feeding experiment. The time required and distance traveled to reach the maze exit, and the number of times that a mouse strayed into blind alleys in the maze were measured. The fatty acid compositions of plasma and brain lipids were measured after the maze behavior experiment. Results: Adult mice in the catechin, DHA-EE, and DHA-EE + catechin diet groups required less time and traveled a shorter distance to reach the maze exit, and strayed into blind alleys fewer times than those in the corresponding lard groups. Among old mice, the DHA-EE + catechin diet group showed an improvement in maze behavior. No marked differences in the brain fatty acid composition between lard and catechin diet groups were observed; in the DHA-EE intake groups, the brain DHA percentage was raised. Conclusion: These results suggest that a simultaneous intake of DHA and catechins may certainly enhance brain function in both adult and old mice.

show abstract

Effect of catechin on the activity and gene expression of superoxide dismutase in cultured rat brain astrocytes

Cited by 38 publications

References 18 publications

Downregulation of Superoxide Dismutase Activity and Gene Expression in Cultured Rat Brain Astrocytes after Incubation with Vitamin C

Downregulation of Superoxide Dismutase Activity and Gene Expression in Cultured Rat Brain Astrocytes after Incubation with Vitamin C

Some aspects of thein vivo neuroprotective capacity of flavonoids: Bioavailability and structure-activity relationship

Effect of Dietary Docosahexaenoic Acid and Catechins on Maze Behavior in Mice

Contact Info

Product

Resources

About