Effect of cationic liposome composition on in vitro cytotoxicity and protective effect on carried DNA

Cortesi, Rita; Esposito, Elisabetta; Menegatti, Enea; Gambari, Roberto; Nastruzzi, Claudio

doi:10.1016/0378-5173(96)04574-7

Cited by 112 publications

(80 citation statements)

References 15 publications

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“…Transfection experiments were carried out in medium not deprived of endogenous estrogenic activity, in the presence of DNA-102 complexed with PC:DOTAP cationic liposomes (Cortesi et al 1996). Cytotoxic effects were not observed: decoy-treated cells showed only slight changes in their morphology (see Fig.…”

Section: The Decoy Effect On Gene Expression In the Te85 Cell Linementioning

confidence: 99%

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Modulation of gene expression in human osteoblasts by targeting a distal promoter region of human estrogen receptor-alpha gene

Lambertini

Penolazzi²,

Sollazzo³

et al. 2002

Journal of Endocrinology

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Estrogen receptor (ER) is expressed during osteoblast differentiation; however, both its functional role in bone metabolism and its involvement in osteoporotic pathogenesis caused by estrogen deficiency are not well understood. Loss of ER gene expression could be one of the mechanisms leading to osteoporosis. Therefore, we investigated a possible modulation of ER gene expression in a human osteoblastic cell line and in four primary osteoblast cultures by using a decoy strategy. Double stranded DNA molecules, mimicking a regulatory region of the ER gene promoter (DNA-102) and acting as a 'silencer' in breast cancer cells, were introduced into osteoblasts as 'decoy' cis-elements to bind and functionally inactivate a putative negative transcription factor, and thus to induce ER gene expression.We found that the DNA-102 molecule was able to specifically bind osteoblast nuclear proteins.Before decoy treatment, absence or variable low levels of ER RNAs in the different cultures were detected. When the cells were transfected with the DNA-102 decoy, an increase in expression of ER and osteoblastic markers, such as osteopontin, was observed, indicating a more differentiated osteoblastic phenotype both in the cell line and in primary cultures. These results showed that the DNA-102 sequence competes with endogenous specific negative transcription factors that may be critical for a decrease in or lack of ER gene transcription. Therefore, osteoblastic transfection with the DNA-102 decoy molecule may be considered a tempting model in a putative therapeutic approach for those pathologies, such as osteoporosis, in which the decrease or loss of ER expression plays a critical role in bone function.

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Section: The Decoy Effect On Gene Expression In the Te85 Cell Linementioning

confidence: 99%

“…The extrusion step was performed in order to obtain unilamellar liposomes with a homogeneous size distribution, as confirmed by freezefracture electron microphotographs (Cortesi et al 1996).…”

Section: Liposome Preparationmentioning

confidence: 99%

Modulation of gene expression in human osteoblasts by targeting a distal promoter region of human estrogen receptor-alpha gene

Lambertini

Penolazzi²,

Sollazzo³

et al. 2002

Journal of Endocrinology

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“…First, previous studies have shown that cationic molecules can have direct cell toxicity; this is especially the case for cationic lipids (29)(30)(31). Moreover, intrapulmonary delivery of cationic lipids and DNA can generate toxic and inflammatory responses on their own (32).…”

Section: Introductionmentioning

confidence: 99%

Incorporation of adenovirus in calcium phosphate precipitates enhances gene transfer to airway epithelia in vitro and in vivo.

Fasbender

Lee²,

Walters³

et al. 1998

J. Clin. Invest.

141

134

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“…The cationic liposomal system, however, has some disadvantages such as low efficiency of transfection due to DNA degradation in lysosomes and strong cyototoxicity. 11 Polycations have been also used for nonviral systems. Among them, polyethylenimine (PEI) has been revealed to be effective to deliver genes, [12][13][14][15][16][17][18][19][20] where genes may be delivered to the cytoplasm via endosomes due to the proton-sponge effect of PEI.…”

Section: Introductionmentioning

confidence: 99%

Polycation liposomes, a novel nonviral gene transfer system, constructed from cetylated polyethylenimine

et al. 2000

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A novel gene transfer system was developed by using liposomes modified with cetylated polyethylenimine (PEI, MW 600). This polycation liposome, PCL, showed remarkable transfection efficiency as monitored by the expression of the GFP reporter gene. Most conventional cationic liposomes require phosphatidylethanolamine or cholesterol as a component, although PCLs did not. Egg yolk phosphatidylcholine-and dipalmitoylphosphatidylcholine-based PCL were as effective as dioleoylphosphatidylethanolaminebased PCLs for gene transfer. Concerning the cytotoxicity against COS-1 cells and hemolytic activity, the PCL was superior to conventional cationic liposome preparations. Fur-

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Effect of cationic liposome composition on in vitro cytotoxicity and protective effect on carried DNA

Cited by 112 publications

References 15 publications

Modulation of gene expression in human osteoblasts by targeting a distal promoter region of human estrogen receptor-alpha gene

Modulation of gene expression in human osteoblasts by targeting a distal promoter region of human estrogen receptor-alpha gene

Incorporation of adenovirus in calcium phosphate precipitates enhances gene transfer to airway epithelia in vitro and in vivo.

Polycation liposomes, a novel nonviral gene transfer system, constructed from cetylated polyethylenimine

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