Effect of cationic polyelectrolytes on the performance of paper diagnostics for blood typing

McLiesh, Heather; Sharman, Scot Andrew; Garnier, Gil

doi:10.1016/j.colsurfb.2015.05.049

Cited by 9 publications

(8 citation statements)

References 23 publications

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“…Poly(amidoamine) epichlorohydrin (PAE) bonds covalently with CNC when heated to >100 °C, 34 thus enhancing the stability of CNCs on a glass surface that has been precoated with this polymer. Aligned CNC was hence fabricated on glass substrates that had been spin coated with a 1 wt % PAE solution (Figure 3a,b).…”

Section: Resultsmentioning

confidence: 99%

Decreasing the Wettability of Cellulose Nanocrystal Surfaces Using Wrinkle-Based Alignment

Prathapan

Berry

Fery

et al. 2017

ACS Appl. Mater. Interfaces

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Cellulose nanocrystals (CNCs) are a particularly appealing format of the natural biopolymer due to their exceptional strength, nanoscale dimensions, and needle-like shape anisotropy. However, CNCs are hydrophilic and hence their wettability makes them impractical for many coating applications, with various approaches using chemical functionalization to overcome this. Here we show that CNC-coated surfaces can be rendered hydrophobic by alignment of the native CNCs using a wrinkled template-mediated printing process. We present a novel and simple method allowing full release of the CNCs from the template and their permanent adsorption into fine patterns onto the surface, thus preventing CNC repositioning during wetting. The aligned CNCs induce strong pinning effects that capture and retain water droplets with high contact angle and large roll-off angles, without becoming susceptible to oil contamination. The fabrication process for these coatings could be achieved by large-scale printing, making them a practical and cost-effective solution to hydrophobic coatings from raw cellulosic materials.

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Section: Resultsmentioning

confidence: 99%

Decreasing the Wettability of Cellulose Nanocrystal Surfaces Using Wrinkle-Based Alignment

Prathapan

Berry

Fery

et al. 2017

ACS Appl. Mater. Interfaces

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“…For better adhesion, the resulting samples comprising random CNC/PAE and aligned CNC/PAE were baked at 105 °C for 5 min, which covalently bonds CNC to PAE. 19 As a control, PAE solution on glass slides was drop-cast after hydrophilizing the slides using plasma treatment for 8−10 min.…”

Section: Methodsmentioning

confidence: 99%

“…Surfaces coated with randomly oriented CNCs were prepared by spin-coating 5 mg/mL CNC dispersion at 2500 rpm for 1 min onto the glass microscope slide coated with PAE. For better adhesion, the resulting samples comprising random CNC/PAE and aligned CNC/PAE were baked at 105 °C for 5 min, which covalently bonds CNC to PAE . As a control, PAE solution on glass slides was drop-cast after hydrophilizing the slides using plasma treatment for 8–10 min.…”

Section: Methodsmentioning

confidence: 99%

“…18 McLiesh et al investigated blood typing via paper diagnostics by using a cationic polyelectrolyte to retain biomolecules on paper. 19 An increase in the concentration and charge density of the cationic polyelectrolyte resulted in a nonlinear retention of red blood cells on paper. The blood group phenotype of red blood cells using flow-through and direct reporting (via paper-based methods) was studied by Then et al 10 Optimization of the process parameters including antibody−antigen reaction time, antibody concentration, etc., was undertaken in order to identify blood groups.…”

Section: Introductionmentioning

confidence: 99%

“…Thin and porous papers were investigated for blood-typing diagnostics wherein the paper structure plays a critical role in the performance of the blood typing assay . McLiesh et al investigated blood typing via paper diagnostics by using a cationic polyelectrolyte to retain biomolecules on paper . An increase in the concentration and charge density of the cationic polyelectrolyte resulted in a nonlinear retention of red blood cells on paper.…”

Section: Introductionmentioning

confidence: 99%

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Surface Engineering of Transparent Cellulose Nanocrystal Coatings for Biomedical Applications

Prathapan

McLiesh

Garnier

2018

ACS Appl. Bio Mater.

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The concept of blood typing diagnostics using blood drops dried onto transparent cellulose nanocrystal thin film (∼35 nm) coatings has been demonstrated. The substrate onto which the blood drops are dried plays an important role in such tests, depending on surface composition, roughness, and wettability. The drying profile of three different fluid dispersions: model latex particles, reagent blood cells, and whole human blood was studied on a range of different surfaces, including cellulose nanocrystals (CNCs), regenerated cellulose, and several hydrophobic polymers, in order to understand the role of surface chemistry, roughness, and fluid dispersion properties. The morphology of these surfaces was investigated using atomic force microscopy, roughness was calculated, and wettability was explored via contact angle measurement. The morphology of dried drops of human blood on different cellulosic surfaces was compared in order to understand the importance of cellulose crystallinity. Well-defined dried blood drops were observed on random and aligned CNC surfaces, facilitating visualization of individual cells. A simple antibody–antigen test was used to demonstrate the effectiveness of the CNC substrate for blood testing, showing high and reproducible selectivity.

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The Role of Paper Chemistry and Paper Manufacture in the Design of Paper-Based Diagnostics

Schabel

Biesalski

2018

Paper-Based Diagnostics

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Effect of cationic polyelectrolytes on the performance of paper diagnostics for blood typing

Cited by 9 publications

References 23 publications

Decreasing the Wettability of Cellulose Nanocrystal Surfaces Using Wrinkle-Based Alignment

Decreasing the Wettability of Cellulose Nanocrystal Surfaces Using Wrinkle-Based Alignment

Surface Engineering of Transparent Cellulose Nanocrystal Coatings for Biomedical Applications

The Role of Paper Chemistry and Paper Manufacture in the Design of Paper-Based Diagnostics

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