Effect of cdri-85/287 on uterine estradiol and progesterone receptor levels/ morphometric measurements during preimplantation period in rat

Gupta, Sandeep; Dhar, J.D.; Dwivedi, Anil Kumar; Bansode, F.W.; Chowdhury, S.R.; Setty, B.S.

doi:10.1080/07435809809135526

Cited by 1 publication

(2 citation statements)

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“…In this context it may be noted that clomiphene does not react with receptors but diminishes receptors, by one of the actions of estrogen on the uterus (6) and/or a disturbance in the balance of estrogen and progesterone as decidualization appears to be the result of estrogenic modulation and continual progesterone action. Moreover, estrogen action stimulates the PRs in rat (23) but reduction in total PRs may reflect the inhibition of action (14). Triphenylethylene anti-estrogens bind to ERs (25) leading to a partial/incomplete activation of them.…”

Section: Discussionmentioning

confidence: 99%

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Changes in uterine ornithine decarboxylase activity and steroid receptor levels during decidualization in the rat induced by CDRI-85/287

Dwivedi

Gupta

Keshri³

et al. 1999

European Journal of Endocrinology

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CDRI-85/287 is an anti-estrogen and interferes with decidualization in the rat uterus. In this study, uterine estrogen receptor (ER) and progesterone receptor (PR) levels were determined during the inhibition of decidualization. The effect of 85/287 on uterine ornithine decarboxylate (ODC) activity (a marker enzyme for decidualization) was also studied, using immature ovariectomized rats divided into four different groups: control, 2.5 mg/kg 85/287 only; 1 mg estradiol only; and 85/287 þ estradiol. Pseudopregnant rats were administered 85/287 (2.5 mg/kg p.o.) on day 3 post-coitum. Deciduoma was induced in one of the uterine horns on day 4 and animals were autopsied 18 h post-traumatization. Both ERs and PRs showed an increase in traumatized horns compared with non-traumatized. In the 85/287-treated uterus, there was a reduction in cytosolic ERs in both traumatized horns. However, nuclear ER and PR levels increased in both horns under the influence of 85/287. Similarly, in a tamoxifen (0.03 mg/kg)-treated group a decline was noticed in cytosolic ER with a mild increase in nuclear PR. Total ER content, expressed per 100 mg DNA, showed a decline in 85/287-or tamoxifentreated rats. However, no significant alterations were observed in total PR levels in non-traumatized horns. In an immature rat model, 85/287 caused a significant (> 50%) reduction in estradiol-induced ODC activity. These findings suggest that the decidualization inhibitory activity of 85/287 may be attributed to inhibition of certain timed biochemical events and genomic/non-genomic actions of estrogens in the rat uterus.

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Section: Discussionmentioning

confidence: 99%

“…After separating the nuclear fraction, the pellet was kept cold (0-4 ЊC) and the supernatant ultracentrifuged at 105 000 g for 60 min at 0-4 ЊC. Cytosolic and nuclear ERs (ER c and ER n ) and PRs (PR c and PR n ) were measured by the method of Martel & Psychoyos (13) with certain modifications as described by Gupta et al (14).…”

Section: Receptor Assaymentioning

confidence: 99%