Effect of ceftiofur on mesophilic anaerobic digestion of dairy manure and the reduction of the cephalosporin-resistance gene cmy-2

Flores-Orozco, Daniel; Patidar, Rakesh; Levin, David B.; Sparling, Richard; Kumar, Ayush; Çiçek, Nazim

doi:10.1016/j.biortech.2019.122729

Cited by 24 publications

(10 citation statements)

References 40 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Considering the existing information with regards to ARGs reductions in anaerobic digestion process, previous research suggested that anaerobic digestion process may have an impact on reducing ARGs. As an example, Flores Orozco [ 57 ] demonstrated that anaerobic digestions removed more than 90% of cephalosporin-resistance gene cmy-2 in dairy manures in a semi-continuous lab-scale anaerobic digester under mesophilic temperature conditions, with a hydraulic retention time of 30 days. Zhang, Yang [ 27 ] found that among 35 studied ARG subtypes, only 8 subtypes were reduced by > 90.0% under thermophilic temperature conditions.…”

Section: Resultsmentioning

confidence: 99%

Degradation of antibiotic resistance genes and mobile gene elements in dairy manure anerobic digestion

et al. 2021

View full text Add to dashboard Cite

Antibiotic resistance genes (ARGs) are emerging contaminants causing serious global health concern. Interventions to address this concern include improving our understanding of methods for treating waste material of human and animal origin that are known to harbor ARGs. Anaerobic digestion is a commonly used process for treating dairy manure, and although effective in reducing ARGs, its mechanism of action is not clear. In this study, we used three ARGs to conducted a longitudinal bench scale anaerobic digestion experiment with various temperatures (28, 36, 44, and 52°C) in triplicate using fresh dairy manure for 30 days to evaluate the reduction of gene abundance. Three ARGs and two mobile genetic elements (MGEs) were studied: sulfonamide resistance gene (sulII), tetracycline resistance genes (tetW), macrolide-lincosamide-streptogramin B (MLSB) superfamily resistance genes (ermF), class 1 integrase gene (intI1), and transposase gene (tnpA). Genes were quantified by real-time quantitative PCR. Results show that the thermophilic anaerobic digestion (52°C) significantly reduced (p < 0.05) the absolute abundance of sulII (95%), intI1 (95%), tnpA (77%) and 16S rRNA gene (76%) after 30 days of digestion. A modified Collins–Selleck model was used to fit the decay curve, and results suggest that the gene reduction during the startup phase of anaerobic digestion (first 5 days) was faster than the later stage, and reductions in the first five days were more than 50% for most genes.

show abstract

Section: Resultsmentioning

confidence: 99%

Degradation of antibiotic resistance genes and mobile gene elements in dairy manure anerobic digestion

et al. 2021

View full text Add to dashboard Cite

show abstract

“…However, there are no consistent results and no key conclusions concerning the fate of ARGs during the anaerobic digestion process [ 153 ]. Some studies have pointed to anaerobic digestion as an alternative to reduce ARG content in different animal manures, although their complete removal has rarely been achieved [ 152 , 154 , 155 , 156 , 157 ]. The efficient removal of ARGs seems to be temperature dependent, since in thermophilic conditions (55 °C) ARG removal rates are higher than in mesophilic conditions (35 °C) [ 152 ].…”

Section: Manure Treatmentmentioning

confidence: 99%

Manure as a Potential Hotspot for Antibiotic Resistance Dissemination by Horizontal Gene Transfer Events

Lima

Domingues

Silva

2020

Veterinary Sciences

140

View full text Add to dashboard Cite

The increasing demand for animal-derived foods has led to intensive and large-scale livestock production with the consequent formation of large amounts of manure. Livestock manure is widely used in agricultural practices as soil fertilizer worldwide. However, several antibiotic residues, antibiotic resistance genes (ARGs) and antibiotic-resistant bacteria are frequently detected in manure and manure-amended soils. This review explores the role of manure in the persistence and dissemination of ARGs in the environment, analyzes the procedures used to decrease antimicrobial resistance in manure and the potential impact of manure application in public health. We highlight that manure shows unique features as a hotspot for antimicrobial gene dissemination by horizontal transfer events: richness in nutrients, a high abundance and diversity of bacteria populations and antibiotic residues that may exert a selective pressure on bacteria and trigger gene mobilization; reduction methodologies are able to reduce the concentrations of some, but not all, antimicrobials and microorganisms. Conjugation events are often seen in the manure environment, even after composting. Antibiotic resistance is considered a growing threat to human, animal and environmental health. Therefore, it is crucial to reduce the amount of antimicrobials and the load of antimicrobial resistant bacteria that end up in soil.

show abstract

“…Ceftiofur (CT), a third‐generation cephalosporin, is widely used for treating cow mastitis due to its excellent antibacterial activity and lack of significant toxic side effects (Wang et al ., 2018 ; Locatelli et al ., 2019 ; Flores‐Orozco et al ., 2020 ). However, the formation of S. xylosus biofilms compromises its effectiveness.…”

Section: Introductionmentioning

confidence: 99%

CD‐g‐CS nanoparticles for enhanced antibiotic treatment of Staphylococcus xylosus infection

Zheng

Zhang

et al. 2021

Microbial Biotechnology

View full text Add to dashboard Cite

Staphylococcus xylosus (S. xylosus)-induced cow mastitis is an extremely serious clinical problem. However, antibiotic therapy does not successfully treat S. xylosus infection because these bacteria possess a strong biofilm formation ability, which significantly reduces the efficacy of antibiotic treatments. In this study, we developed ceftiofur-loaded chitosan grafted with β-cyclodextrins (CD-g-CS) nanoparticles (CT-NPs) using host-guest interaction. These positively charged nanoparticles improved bacterial internalization, thereby significantly improving the effectiveness of antibacterial treatments for planktonic S. xylosus. Moreover, CT-NPs effectively inhibited biofilm formation and eradicated mature biofilms. After mammary injection in a murine model of S. xylosus-induced mastitis, CT-NPs significantly reduced bacterial burden and alleviated inflammation, thereby achieving optimized therapeutic efficiency for S. xylosus infection. In conclusion, this treatment strategy could improve the efficiency of antibiotic therapeutics and shows great potential in the treatment of S. xylosus infections.

show abstract

Effect of ceftiofur on mesophilic anaerobic digestion of dairy manure and the reduction of the cephalosporin-resistance gene cmy-2

Cited by 24 publications

References 40 publications

Degradation of antibiotic resistance genes and mobile gene elements in dairy manure anerobic digestion

Degradation of antibiotic resistance genes and mobile gene elements in dairy manure anerobic digestion

Manure as a Potential Hotspot for Antibiotic Resistance Dissemination by Horizontal Gene Transfer Events

CD‐g‐CS nanoparticles for enhanced antibiotic treatment of Staphylococcus xylosus infection

Contact Info

Product

Resources

About