Effect of Cell Confluence on Production of Cloned Mice Using an Inbred Embryonic Stem Cell Line1

Gao, Shaorong; McGarry, Michelle H.; Ferrier, Tricia; Pallante, Benedetta A.; Gasparrini, B.; Fletcher, Judy; Harkness, Linda; Sousa, Paul A. De; McWhir, Jim; Wilmut, Ian

doi:10.1095/biolreprod.102.005819

Cited by 52 publications

(37 citation statements)

References 29 publications

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“…The high reprogrammability (the ability of the genome to be reprogrammed) of the ES cell genome might be attributed to the consecutive expression of Oct3/4, a key upstream factor required for normal early embryonic development [38]. Better cell cycle synchrony between the ES cell nucleus and the recipient ooplasm can be achieved by confluent culture [39] or through cell cycle arrest at the M-phase with nocodazole treatment [40].…”

Section: What Determines Genomic Reprogrammability? Lessons From Micementioning

confidence: 99%

Recent advancements in cloning by somatic cell nuclear transfer

Ogura

Inoue

Wakayama

2013

Phil. Trans. R. Soc. B

189

178

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Somatic cell nuclear transfer (SCNT) cloning is the sole reproductive engineering technology that endows the somatic cell genome with totipotency. Since the first report on the birth of a cloned sheep from adult somatic cells in 1997, many technical improvements in SCNT have been made by using different epigenetic approaches, including enhancement of the levels of histone acetylation in the chromatin of the reconstructed embryos. Although it will take a considerable time before we fully understand the nature of genomic programming and totipotency, we may expect that somatic cell cloning technology will soon become broadly applicable to practical purposes, including medicine, pharmaceutical manufacturing and agriculture. Here we review recent progress in somatic cell cloning, with a special emphasis on epigenetic studies using the laboratory mouse as a model.

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Section: What Determines Genomic Reprogrammability? Lessons From Micementioning

confidence: 99%

Recent advancements in cloning by somatic cell nuclear transfer

Ogura

Inoue

Wakayama

2013

Phil. Trans. R. Soc. B

189

178

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“…For reasons not fully understood, the 129 strain is unique as an easy source for spontaneous teratocarcinomas and derivation of embryo carcinoma and ES cell lines. It is also the only inbred strain from which ES cell-derived viable clones have been obtained Amano et al, 2001;Gao et al, 2003b), although at lower efficiency than with 129-hybrids (Table 1). Among the F1-hybrids, only one cell line, v6.5, has given extraordinarily high cloning efficiencies (up to 33%) in some cases , but not in others (Humpherys et al, 2001), possibly depending on which targeted sub-clone and passage number was used for NT.…”

Section: Embryonic Stem Cellsmentioning

confidence: 99%

“…An alternative method of inducing quiescence, culture to confluence, has also been tested and significantly increases cloning efficiency with ES cells (E14), however, this may be a cell cycle-, or even cell typeindependent effect (Gao et al, 2003b). Confluent cultures contain a larger proportion of smaller cells, perhaps affecting cloning efficiency because nuclei from smaller cells are less likely to be damaged during NT by direct microinjection.…”

Section: Embryonic Stem Cellsmentioning

confidence: 99%

Donor cell differentiation, reprogramming, and cloning efficiency: Elusive or illusive correlation?

Oback

Wells

2006

Molecular Reproduction Devel

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Compared to other assisted reproductive technologies, mammalian nuclear transfer (NT) cloning is inefficient in generating viable offspring. It has been postulated that nuclear reprogramming and cloning efficiency can be increased by choosing less differentiated cell types as nuclear donors. This hypothesis is mainly supported by comparative mouse cloning experiments using early blastomeres, embryonic stem (ES) cells, and terminally differentiated somatic donor cells. We have re-evaluated these comparisons, taking into account different NT procedures, the use of donor cells from different genetic backgrounds, sex, cell cycle stages, and the lack of robust statistical significance when post-blastocyst development is compared. We argue that while the reprogrammability of early blastomeres appears to be much higher than that of somatic cells, it has so far not been conclusively determined whether differentiation status affects cloning efficiency within somatic donor cell lineages. Mol. Reprod. Dev. 74: 646-654, 2007. ß

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“…The potential of nuclear-transferred oocytes to develop into young might be different among cell lines as ES cells with a h y b r i d g e n e t i c b a c k g r o u n d h a v e h i g h developmental potential when they are transferred to oocytes or injected into tetraploid blastocysts [6]. The genetic background of ES cells is not the only factor underlying the low potential of nucleartransferred oocytes to develop into young because cell confluence and cell passages of ES cells are also important factors [7].…”

mentioning

confidence: 99%

Effects of Nuclear Transfer Procedures on ES Cell Cloning Efficiency in the Mouse

Yabuuchi

Yasuda

Kato

et al. 2004

Journal of Reproduction and Development

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Abstract. Enucleated oocytes receiving mouse embryonic stem (ES) cells develop into fertile young.The developmental potential to young is low, however, and the rate of postnatal death is high. We examined the effect of various nuclear transfer procedures on the in vitro and in vivo developmental potential of nuclear-transferred oocytes. The potential of oocytes receiving ES cells at M phase to develop into blastocysts after fusion by Sendai virus was high compared with that after direct injection (67% vs. 30%). The developmental potential of oocytes receiving ES cells at the M phase is higher than that of oocytes receiving ES cells at the G1 phase (30-67% vs. 2-5%). Developmental ability to live young was low in all groups (0-4%). Different activation protocols affected the potential to develop into blastocysts to a different extent (27-62%), but did not affect the potential to develop into live young (0-3%). The present study demonstrated that the various conditions examined did not affect the potential of nuclear-transferred oocytes receiving ES cells to develop into live young or the incidence of postnatal death.

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Effect of Cell Confluence on Production of Cloned Mice Using an Inbred Embryonic Stem Cell Line1

Cited by 52 publications

References 29 publications

Recent advancements in cloning by somatic cell nuclear transfer

Recent advancements in cloning by somatic cell nuclear transfer

Donor cell differentiation, reprogramming, and cloning efficiency: Elusive or illusive correlation?

Effects of Nuclear Transfer Procedures on ES Cell Cloning Efficiency in the Mouse

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