Effect of cell‐phone radiofrequency on angiogenesis and cell invasion in human head and neck cancer cells

Alahmad, Yaman M.; Aljaber, Mohammed; Saleh, Alaaeldin; Yalcin, Huseyin C.; Aboulkassim, Tahar; Yasmeen, Amber; Batist, Gerald; Moustafa, Ala-Eddin Al

doi:10.1002/hed.25210

Cited by 2 publications

(1 citation statement)

References 22 publications

(50 reference statements)

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“…For instance, it can aid in understanding the mechanisms of cancer metastasis as well as the effect of certain inhibitors, drugs, or radiation on cancer metastasis using various cell types. In the earlier studies, using the chicken embryo CAM assay and in vitro cell culture, our group showed that cell-phone radio frequency can promote angiogenesis and cancer cell invasion [41]; additionally, the effect of cell-phone radio frequency on cancer cell metastasis can be verified using this model. Our ex ovo model also overcomes some of the limitations of widely-used zebrafish models [42].…”

Section: Discussionmentioning

confidence: 93%

A novel in ovo model to study cancer metastasis using chicken embryos and GFP expressing cancer cells

Augustine

Alhussain

Hasan

et al. 2019

Bosn J of Basic Med Sci

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Cancer metastasis is the leading cause of cancer-related mortality worldwide. To date, several in vitro methodologies have been developed to understand the mechanisms of cancer metastasis and to screen various therapeutic agents against it. Nevertheless, mimicking an in vivo microenvironment in vitro is not possible; while in vivo experiments are complex, expensive and bound with several regulatory requirements. Herein, we report a novel in ovo model that relies on chicken embryo to investigate cancer cell invasion and metastasis to various organs of the body. In this model, we directly injected green fluorescent protein (GFP) expressing cancer cells to the heart of chicken embryo at 3 days of incubation, then monitored cell migration to various organs. To this end, we used a simple tissue processing technique to achieve rapid imaging and quantification of invasive cells. We were able to clearly observe the migration of GFP expressing cancer cells into various organs of chicken embryo. Organ specific variation in cell migration was also observed. Our new slide pressing based tissue processing technique improved the detectability of migrated cells. We herein demonstrate that the use of GFP expressing cancer cells allows easy detection and quantification of migrated cancer cells in the chicken embryo model, which minimizes the time and effort required in this types of studies compared to conventional histopathological analysis. In conclusion, our investigation provides a new cancer metastasis model that can be further improved to include more complex aspects, such as the use of multiple cell lines and anti-metastatic agents, thus opening new horizons in cancer biology and pharmaceutical research.

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Section: Discussionmentioning

confidence: 93%

A novel in ovo model to study cancer metastasis using chicken embryos and GFP expressing cancer cells

Augustine

Alhussain

Hasan

et al. 2019

Bosn J of Basic Med Sci

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Assessing the effect of selenium on cyclin D1 level and nuclear factor kappa b activity in NIH/3T3 fibroblast cells at 2100 MHz electromagnetic field exposure

Ergun,

Ozsobaci,

Yilmaz

et al. 2023

Electromagnetic Biology and Medicine

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Purpose: Although there are various studies on the biological effects of the 2100 MHz electromagnetic eld (EMF), which is among the mobile phone frequencies, there are con icting results in the literature on this subject. Materials andMethods: The present study was designed to assess the effect of 200 nM selenium (Se) on cell viability% [trypan blue], cell cycle biomarker [cyclin D1] and the transcription factor [nuclear factor kappa b (NF-κB)] in NIH/3T3 broblast cells exposed to 2100 MHz mobile phone frequency. Cells were divided to following groups: Control, sham control, 2100 MHz EMF, and 200 nM Se + 2100 MHz EMF for 2 h. And we measurement cell viability%, cyclin D1 level and NF-κB activity. Results and Conclusions: While of cell viability% decreased in 2100 MHz EMF group compared to control group, cell viability% increased in 200 nM Se + 2100 MHz EMF group compared to 2100 MHz EMF. It was also shown that while cyclin D1 level and NF-κB activity increased signi cantly in the 2100 MHz EMF group compared to the control group, the same parameters decreased in the 200 nM Se + 2100 MHz EMF group compared to the 2100 MHz EMF group. Although future studies will be required to investigate the biological effects of EMF emitted by mobile phones, our results provide insight into the molecular mechanisms underlying selenium's protective function and e cacy against 2100 MHz EMF exposure.

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Effect of cell‐phone radiofrequency on angiogenesis and cell invasion in human head and neck cancer cells

Abstract: Our investigation reveals that cell-phone radiofrequency could enhance head and neck cancer by stimulating angiogenesis and cell invasion via Erk1/Erk2 activation.

Cited by 2 publications

References 22 publications

A novel in ovo model to study cancer metastasis using chicken embryos and GFP expressing cancer cells

A novel in ovo model to study cancer metastasis using chicken embryos and GFP expressing cancer cells

Assessing the effect of selenium on cyclin D1 level and nuclear factor kappa b activity in NIH/3T3 fibroblast cells at 2100 MHz electromagnetic field exposure

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