Effect of cell seeding density on matrix‐forming capacity of meniscus fibrochondrocytes and nasal chondrocytes in meniscus tissue engineering

Abstract

The presence of intact menisci is imperative for the proper function of the knee joint. Meniscus injuries are often treated by the surgical removal of the damaged tissue, which increases the likelihood of post‐traumatic osteoarthritis. Tissue engineering holds great promise in producing viable engineered meniscal tissue for implantation using the patient's own cells; however, the cell source for producing the engineered tissue is unclear. Nasal chondrocytes (NC) possess many attractive features for engineering meniscus. However, in order to validate the use of NC for engineering meniscus fibrocartilage, a thorough comparison of NC and meniscus fibrochondrocytes (MFC) must be considered. Our study presents an analysis of the relative features of NC and MFC and their respective chondrogenic potential in a pellet culture model. We showed considerable differences in the cartilage tissue formed by the two different cell types. Our data showed that NC were more proliferative in culture, deposited more extracellular matrix, and showed higher expression of chondrogenic genes than MFC. Overall, our data suggest that NC produce superior cartilage tissue to MFC in a pellet culture model. In addition, NCs produce higher quality cartilage tissue at higher cell seeding densities during cell expansion.

Osteogenic differentiation of BMSCs on MoS2 composite nanofibers with different cell seeding densities

“…The cell seeding densities of the studied groups were 5.0 × 10 4 cells/cm 2 , 2.5 × 10 4 cells/ cm 2 , 1.0 × 10 4 cells/cm 2 , 5.0 × 10 3 cells/cm 2 and 2.5 × 10 3 cells/cm 2 . The cell densities were selected from previously published research studies (Anderson-Baron et al 2020;Eyckmans et al 2012;Mehrian et al 2020;Singh et al 2017;Wu et al 2020). The corresponding cells were then seeded onto MoS 2 /PAN in each well (400 μL of medium per well).…”

Three-dimensional culture and chondrogenic differentiation of mesenchymal stem cells in interconnected collagen scaffolds

“…Cartilage tissue engineering has been developed as an attractive technique to heal cartilage defects [1,2]. It has been broadly used for the regeneration of cartilage tissues to treat defects such as articular joint cartilage, nasal cartilage, auricular cartilage, tracheal cartilage, meniscus and intervertebral disc defects [3][4][5][6]. Different cell sources, such as chondrocytes and stem cells, are frequently used [7][8][9].…”

Table_8.DOCX

“…Because PTHrP has been demonstrated to suppress hypertrophic differentiation of chondrocytes, we sought to examine the use of PTHrP to prevent the hypertrophy and subsequence calcification of cocultured NC and BM-MSC-derived tissue engineered cartilage. Tissue engineered microtissue cartilage constructs were formed from cell aggregate pellet cultures of human NC, BM-MSC, and cocultures of NC and BM-MSC (Anderson-Baron et al, 2020). Cocultured microtissue pellets were treated with increasing concentrations of recombinant human PTHrP.…”