Effect of cell-seeding density on the proliferation and gene expression profile of human umbilical vein endothelial cells within ex vivo culture

Heng, Boon Chin; Bezerra, Paula Porto; Preiser, Peter R.; Law, S.K. Alex; Xia, Yun; Boey, Freddy; Venkatraman, Subbu S.

doi:10.3109/14653249.2010.542455

Cited by 34 publications

(22 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, the difference with bare ePET was less marked, probably due to the fact that large numbers of cells were seeded, 10 6 cells per mat. It has been reported that the influence of the substrate on cell adhesion can be reduced when cell density is very high, possibly due to cell–cell interactions that may dominate over those with the substrate. After exposure to shear, cell densities were significantly greater on ePET‐LPPE:N than on bare (ePET) samples ( p < 0.05), as also confirmed by SEM observation (Figure ).…”

Section: Resultsmentioning

confidence: 99%

Electrospun Nanofiber Scaffolds and Plasma Polymerization: A Promising Combination Towards Complete, Stable Endothelial Lining for Vascular Grafts

Savoji

Hadjizadeh

Maire

et al. 2014

Macromolecular Bioscience

View full text Add to dashboard Cite

In the quest to reduce risk of thrombosis in vascular grafts, it is essential to provide a surface with morphological and mechanical properties close to those of the extracellular matrix beneath the luminal endothelium, and to favor the growth of a confluent, stable monolayer of endothelial cells. This is accomplished here by combining electrospun poly(ethylene terephthalate) (PET) mats with an amine-rich thin plasma-polymerized coating, designated "L-PPE:N." Its deposition does not modify the open, highly porous mats and leads only to small changes in mechanical properties. L-PPE:N significantly improves the adhesion and growth of human umbilical vein endothelial cells (HUVEC) and their resistance to flow-induced shear stress. These properties favor the formation of desired confluent HUVEC monolayers on the topmost surface, unlike conventional vascular grafts (ePTFE or woven PET), where cells migrate inside the material. This combination is therefore highly advantageous for the pre-endothelialization of the luminal side of small-diameter vascular prostheses.

show abstract

Section: Resultsmentioning

confidence: 99%

Electrospun Nanofiber Scaffolds and Plasma Polymerization: A Promising Combination Towards Complete, Stable Endothelial Lining for Vascular Grafts

Savoji

Hadjizadeh

Maire

et al. 2014

Macromolecular Bioscience

View full text Add to dashboard Cite

show abstract

“…This could result in lower proliferation rates than expected. 45 Although cell counts were initially lower on the 3D scaffolds, the maturation of MG63 and human osteoblasts was enhanced. This finding is consistent with previous studies reporting that osteoblastic proliferation and differentiation are reversely correlated, which means when proliferation rate is high, the differentiation state is reduced.…”

Section: Discussionmentioning

confidence: 96%

Role of integrin α₂β₁ in mediating osteoblastic differentiation on three‐dimensional titanium scaffolds with submicron‐scale texture

Wang

Schwartz

Gittens

et al. 2014

J Biomedical Materials Res

View full text Add to dashboard Cite

Hierarchical surface roughness of titanium and titanium alloy implants plays an important role in osseointegration. In vitro and in vivo studies show greater osteoblast differentiation and bone formation when implants have submicron-scale textured surfaces. In this study, we tested the potential benefit of combining a submicron-scale textured surface with three-dimensional (3D) structure on osteoblast differentiation and the involvement of an integrin-driven mechanism. 3D titanium scaffolds were made using orderly oriented titanium meshes and microroughness was added to the wire surface by acid-etching. MG63 and human osteoblasts were seeded on 3D scaffolds and 2D surfaces with or without acid etching. At confluence, increased osteocalcin, vascular endothelial growth factor, osteoprotegerin (OPG), and alkaline phosphatase (ALP) activity were observed in MG63 and human osteoblasts on 3D scaffolds in comparison to 2D surfaces at the protein level, indicating enhanced osteoblast differentiation. To further investigate the mechanism of osteoblast-3D scaffold interaction, the role of integrin α2β1 was examined. The results showed β1 and α2β1 integrin silencing abolished the increase in osteoblastic differentiation markers on 3D scaffolds. Time course studies showed osteoblasts matured faster in the 3D environment in the early stage of culture, while as cells proliferated, the maturation slowed down to a comparative level as 2D surfaces. After 12 days of postconfluent culture, osteoblasts on 3D scaffolds showed a second-phase increase in ALP activity. This study shows that osteoblastic differentiation is improved on 3D scaffolds with submicron-scale texture and is mediated by integrin α2β1.

show abstract

“…The difference in the experimental conditions, particularly initial cell seeding densities, could help explain the variable results of different studies. In particular, a recent study showed that HUVEC proliferation depends highly on the cell seeding density, and even a small increase of cell density might inhibit proliferation [26]. The diversity of nicotine effects might also be explained by the fact that ECs express a variety of nicotinic acetylcholine receptors which might differently regulate various cellular functions [27].…”

Section: Discussionmentioning

confidence: 99%

Effect of Nicotine and Porphyromonas gingivalis Lipopolysaccharide on Endothelial Cells In Vitro

Andrukhov

Tang

et al. 2014

PLoS ONE

View full text Add to dashboard Cite

Smoking is considered a significant risk factor for both periodontal disease and cardiovascular disease (CVD). Endothelial cells play an important role in the progression of both diseases. In the present study, we investigated in vitro the impact of nicotine on functional properties of human umbilical vein endothelial cells (HUVECs) stimulated with lipopolysaccharide (LPS) of periodontal pathogen Porphyromonas gingivalis. HUVECs were stimulated with different concentrations of nicotine (10 µM-10 mM) and/or P. gingivalis LPS. Expression levels of intercellular adhesion molecule-1, vascular cell adhesion molecule-1, E-selectin, monocyte chemoattractant protein 1, and interleukin-8 were measured on both gene and protein levels. Cell proliferation/viability, apoptosis, and migration were also investigated. Nicotine at a concentration of 10 mM significantly decreased P. gingivalis LPS-induced expression of all investigated proteins after 4 h stimulation, while lower nicotine concentrations had no significant effect on protein expression with or without P. gingivalis LPS. Proliferation/viability of HUVECs was also significantly inhibited by 10-mM nicotine but not by lower concentrations. Migration of HUVECs was significantly decreased by nicotine at concentrations of 1–10 mM. Nicotine at a concentration similar to that observed in the serum of smokers had no significant effect on the functional properties of HUVECs. However, high concentrations of nicotine, similar to that observed in the oral cavity of smokers, inhibited the inflammatory response of HUVECs. This effect of nicotine might be associated with decreased gingival bleeding indices in smoking periodontitis patients.

show abstract

Effect of cell-seeding density on the proliferation and gene expression profile of human umbilical vein endothelial cells within ex vivo culture

Cited by 34 publications

References 34 publications

Electrospun Nanofiber Scaffolds and Plasma Polymerization: A Promising Combination Towards Complete, Stable Endothelial Lining for Vascular Grafts

Electrospun Nanofiber Scaffolds and Plasma Polymerization: A Promising Combination Towards Complete, Stable Endothelial Lining for Vascular Grafts

Role of integrin α₂β₁ in mediating osteoblastic differentiation on three‐dimensional titanium scaffolds with submicron‐scale texture

Effect of Nicotine and Porphyromonas gingivalis Lipopolysaccharide on Endothelial Cells In Vitro

Contact Info

Product

Resources

About

Effect of cell-seeding density on the proliferation and gene expression profile of human umbilical vein endothelial cells within ex vivo culture

Cited by 34 publications

References 34 publications

Electrospun Nanofiber Scaffolds and Plasma Polymerization: A Promising Combination Towards Complete, Stable Endothelial Lining for Vascular Grafts

Electrospun Nanofiber Scaffolds and Plasma Polymerization: A Promising Combination Towards Complete, Stable Endothelial Lining for Vascular Grafts

Role of integrin α2β1 in mediating osteoblastic differentiation on three‐dimensional titanium scaffolds with submicron‐scale texture

Effect of Nicotine and Porphyromonas gingivalis Lipopolysaccharide on Endothelial Cells In Vitro

Contact Info

Product

Resources

About

Role of integrin α₂β₁ in mediating osteoblastic differentiation on three‐dimensional titanium scaffolds with submicron‐scale texture