Effect of cellulase and lactic acid bacteria on the fermentation quality, carbohydrate conversion, and microbial community of ensiling oat with different moisture contents

Xu, Jinyi; Zhang, Keyi; Yang, Lin; Li, Mengxin; Wang, Xuekai; Yu, Qiang; Sun, Hong; Cheng, Qiming; Xie, Yixiao; Wang, Chunmei; Li, Ping; Chen, Chao; Fang, Yang; Zheng, Yulong

doi:10.3389/fmicb.2022.1013258

Cited by 17 publications

(20 citation statements)

References 51 publications

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“…HMEC from each treatment in this experiment were individually aggregated within a narrow range, indicating good reproducibility of the samples and significant separation and differences in the distribution and composition of the microbial community across treatments. This result suggested that different total classes of additives as well as different moisture could significantly affect the microbial composition of HMEC, which is in agreement with the results of Xu et al (2022) about the effect of moisture and additives on the microbial composition of the community in silage.…”

Section: Discussionsupporting

confidence: 90%

Effect of additives and moisture on the fermentation quality and bacterial community of high moisture ear corn

Li,

Shang

et al. 2023

Front. Microbiol.

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Maize (Zea mays L) is one of the most widely cultivated crops used as energy feeds. The aim of this study was to evaluate the effects of two lactic acid bacteria additives on the fermentation quality and bacterial community of high moisture ear corn (HMEC) silage at different moisture levels. The study utilized corn kernels and cobs harvested at the stage of complete ripeness as the primary material. The cob was crushed and divided into three treatment groups: an untreated control group (CK), a group treated with a mixture of Lactobacillus plantarum and Lactobacillus brucei (TQ), or a group treated with a mixture of Lactococcus lactis and Lactobacillus brucei (KT). Moisture contents were adjusted to 37.5% (L), 42.5% (M) or 47.5% (H) and then silaged for 180 days. Compared to CK, TQ, and KT elevated the dry matter, crude protein, starch, lactic and acetic acid content of HMEC and reduced the pH, neutral detergent fiber, acid detergent fiber and ammonia nitrogen content (p < 0.05). Even though both additives improved the bacterial community structure after fermentation, KT experienced the greater enhancement. At a phylum and genus level, KT had the higher relative abundance of Firmicutes and Lactobacillus, respectively. Compared with the group of 37.5% (L) moisture content, the 42.5% (M) and 47.5% moisture content (H) group increased lactic acid, acetic acid and ammonia nitrogen concentrations and reduced the pH value (p < 0.05). In conclusion, the addition of TQ and KT at the appropriate moisture content might be helpful for producing high-quality HMEC. Among the three moisture contents, 42.5% (M) moisture content provides the best silage qualities.

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Section: Discussionsupporting

confidence: 90%

Effect of additives and moisture on the fermentation quality and bacterial community of high moisture ear corn

Li,

Shang

et al. 2023

Front. Microbiol.

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“…This indicated that the fermentation of the mixed silage of king grass and rice straw was dominated by homofermentation. Consistently, Liu et al showed that the abundance of Lactiplantibacillus was positively correlated with WSC content [ 51 ], and Lactiplantibacillus had a positive effect on nutrient preservation [ 52 ].…”

Section: Discussionmentioning

confidence: 93%

Effects of Cellulase and Xylanase on Fermentation Characteristics, Chemical Composition and Bacterial Community of the Mixed Silage of King Grass and Rice Straw

Qiu,

Liu,

Diao

et al. 2024

Microorganisms

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This study was to investigate the effects of cellulase and xylanase on fermentation characteristics, nutrient composition and the bacterial community of the mixed silage of king grass and rice straw. Lab-scale bag silage was produced and seven groups were studied: blank control (CK); added 1%, 2% cellulase (CE1, CE2); added 1%, 2% xylanase (XY1, XY2); and added 0.5% cellulase +0.5% xylanase, 1% cellulase +1% xylanase (CX1, CX2). The results showed that the application of additives in six treated groups exerted a positive effect on lactic acid (LA) content and their pH values decreased significantly (p < 0.05). The addition of cellulase and xylanase decreased (p < 0.05) the content of neutral detergent fiber (NDF) and acid detergent fiber (ADF) significantly and increased (p < 0.01) the crude protein (CP) and water-soluble carbohydrate (WSC) content. Filter paper enzyme activity (FPA) declined and xylanase activity (XA) intensified (p < 0.05) as ensiling was prolonged, where most of the enzymatic treatments (especially XY2, CX2) resulted in increased enzyme activities. Moreover, the addition of cellulase and xylanase reduced the abundance of harmful bacteria such as Acinetobacter and Klebsiella and increased the abundance of lactic acid bacteria such as Lacticaseseibacillus, Lactiplantibacillus. In conclusion, the addition of cellulase and xylanase would improve fermentation quality and nutrient preservation via altering the bacterial community, with 1% cellulase or complex enzyme best.

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“…The relative abundance of Proteobacteria was lower in the PAA 2000 and CTC 500 groups than in the control group. Proteobacteria play a pivotal role in fermentation [56]. Proteobacteria consist mostly of pathogenic bacteria [57].…”

Section: Discussionmentioning

confidence: 99%

Dietary Palygorskite-Based Antibacterial Agent Supplementation as an Alternative to Antibiotics Improves Growth Performance, Blood Parameters, and Rumen Microbiota in Sheep

Liu

Zeng

et al. 2023

Antibiotics

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This research aimed to investigate the effects of a palygorskite-based antibacterial agent (PAA) as an alternative to antibiotics on growth performance, blood parameters, and rumen microbiota in sheep. A total of 120 sheep were randomly divided into five groups of six replicates with four sheep each. Sheep were fed a basal diet, an antibiotic diet supplemented with 500 g/t chlortetracycline (CTC), and a basal diet supplemented with 500, 1000, and 2000 g/t PAA for 80 d, respectively. Supplementation with 2000 g/t PAA and 500 g/t CTC increased the average daily gain (ADG) of sheep compared with the control group (p < 0.05). Diets supplemented with 2000 g/t PAA and 500 g/t CTC reduced (p < 0.05) the feed:gain ratio (F/G ratio) in the overall periods. Dietary supplementation with 1000 g/t PAA significantly increased albumin and total protein (p < 0.05). A significant positive correlation was found between growth hormone concentration and PAA supplementation (p < 0.05). In addition, compared to the control group, the CTC group had higher growth hormone concentration and lower lipopolysaccharide concentration (p < 0.05). No difference was observed between the five groups in terms of rumen fermentation characteristics (p > 0.05). At the phylum level, the relative abundance of Proteobacteria was lower in the PAA 2000 and CTC 500 groups than in the control and PAA 500 groups (p < 0.05). At the genus level, a significant decrease (p < 0.05) in the relative abundance of RuminococcaceaeUCG-010 was observed in the PAA 1000, PAA 2000, and CTC 500 groups compared with that in the control group. In addition, the relative abundance of Prevotella1 (p < 0.05) was higher in the PAA 2000 group than in the control group. These findings indicate that dietary supplementation with PAA has ameliorative effects on growth performance, blood parameters, and rumen microbiota, with an optimal dosage of 2000 g/t for sheep.

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Effect of cellulase and lactic acid bacteria on the fermentation quality, carbohydrate conversion, and microbial community of ensiling oat with different moisture contents

Cited by 17 publications

References 51 publications

Effect of additives and moisture on the fermentation quality and bacterial community of high moisture ear corn

Effect of additives and moisture on the fermentation quality and bacterial community of high moisture ear corn

Effects of Cellulase and Xylanase on Fermentation Characteristics, Chemical Composition and Bacterial Community of the Mixed Silage of King Grass and Rice Straw

Dietary Palygorskite-Based Antibacterial Agent Supplementation as an Alternative to Antibiotics Improves Growth Performance, Blood Parameters, and Rumen Microbiota in Sheep

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