Effect of cellulase on the UCST behavior of sulfobetaine zwitterionic surfactants and the cellulase recovery mechanism

Li, Feiyun; Qin, Feiyang; Cai, Cheng; Pang, Yongqiang; Liu, Weifeng; Li, Qiong; Li, Hongming; Qiu, Xueqing

doi:10.1039/d0se01158d

Cited by 5 publications

(7 citation statements)

References 21 publications

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“…PPSP 10 -3 has a sensitive UCST response with r of 75.1% at 25 °C. Previous studies have shown that all sulfobetaine compounds co-precipitated with cellulase mainly through hydrophobic interaction at lower temperatures. , As predicted, cellulase was recovered at room temperature by adding PPSP 10 -3 in this work. First, the effect of the amount of PPSP 10 -3 on the recovery rate of cellulase was investigated, and the result is shown in Figure .…”

Section: Resultsmentioning

confidence: 99%

“…PPSP could only be used to recover about 25% of cellulase. This is due to the introduction of TPEG in PPSP, which increased the hydrophilicity of the polymer. , …”

Section: Resultsmentioning

confidence: 99%

“…On the basis of direct addition of recovered cellulase, 0, 10, 20, 30, 40, 50, 60, and 70% of initial enzyme loadings (free CTec2) were supplemented, respectively, and SED@48 h was measured again. The cellulase recovery rate was expressed by the amount of saved cellulase, by comparing with SED of the first enzymatic hydrolysis. , …”

Section: Methodsmentioning

confidence: 99%

“…Li et al reported that octadecyl sulfopropyl betaine, sulfobetaine polymer (PSPE) had upper critical solution temperature (UCST) response, which dissolved at higher temperature and precipitated at lower temperature. , Cellulase was recovered at room temperature by using temperature response performances of PSPE . However, the binding force between PSPE and cellulase was weak at lower temperature, and PSPE could not enhance lignocellulosic hydrolysis, which limits the application of PSPE in the recovery of cellulase.…”

Section: Introductionmentioning

confidence: 99%

“…The cellulase recovery rate was expressed by the amount of saved cellulase, by comparing with SED of the first enzymatic hydrolysis. 15,16 Analysis Method. A Fourier infrared spectrometer (Nicolet 5700, Thermoelectric Company, USA) and Bruker AV 400 (Brooke, Germany) were used to characterize PSPP, respectively.…”

Section: ■ Introductionmentioning

confidence: 99%

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Synthesis of Recyclable UCST-Type Copolymer PPSP and Its Application in Enhancing Lignocellulosic Enzymatic Hydrolysis and Recycling Cellulase

Zhang

et al. 2023

ACS Sustainable Chem. Eng.

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The cost of lignocellulosic enzymatic hydrolysis can be reduced by adding additives with upper critical solution temperature (UCST) response. A recyclable UCST-type copolymer PPSP was synthesized, for enhancing lignocellulosic enzymatic hydrolysis and the cellulase recovery effect of substrate reabsorption. For the hydrolysis system of corncob residue without adding PPSP, only 50% of cellulase amount was saved at a room temperature of 25 °C by adding the fresh corncob residue. The substrate enzymatic digestion efficiency for 72 h was increased by 1.3 times with only adding 0.5 g/L PPSP 10 -3. After the hydrolysis, 70% of cellulase amount was saved at 25 °C by using the above method. Both lignocellulosic hydrolysis and recovery of cellulase performances were improved by adding PPSP. This may be attributed to the fact that PPSP preferentially adsorbed on lignin to form a hydration membrane to reduce the no-productive adsorption of cellulase and enhanced lignocellulosic enzymatic hydrolysis, while increasing the concentration of free cellulase in the system and strengthening recovery of cellulase. This work will provide one way to reduce the cost of lignocellulosic enzymatic hydrolysis.

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Section: Resultsmentioning

confidence: 99%

“…PPSP could only be used to recover about 25% of cellulase. This is due to the introduction of TPEG in PPSP, which increased the hydrophilicity of the polymer. , …”

Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: ■ Introductionmentioning

confidence: 99%

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Synthesis of Recyclable UCST-Type Copolymer PPSP and Its Application in Enhancing Lignocellulosic Enzymatic Hydrolysis and Recycling Cellulase

Zhang

et al. 2023

ACS Sustainable Chem. Eng.

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Rational design of temperature‐responsive binary polymers containing sulfobetaine and quaternary ammonium fragments for application in lignocellulosic hydrolysis

Pan,

Li,

Yuan

et al. 2024

J of Applied Polymer Sci

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Enzymatic strengthening and cellulase recovery are effective ways for improving the economy of lignocellulosic hydrolysis. A series of temperature‐responsive quaternary ammonium salt type enzymatic hydrolysis promoters (PSDM) were obtained by aqueous‐solution free radical polymerization. PSDM dissolved under lignocellulosic enzymatic hydrolysis conditions (50°C). After hydrolysis, it co‐precipitated with cellulase when cooling. PSDM had significantly improved hydrolysis and recycling enzyme properties. The efficiency of high‐solid substrate enzymatic hydrolysis of corncob residues improved to 1.2 times, and 50% of the cellulase amount was saved when adding 1.5 g/L of PSDM1 to the system. Hydrophobic binding may be the main driving force for co‐precipitation between PSDM and cellulase. In this work, regulation of glucose yield and cellulase consumption were realized by adjusting the ratio of positive and negative charges in PSDM. This work provides a new idea for reducing the cost of technology for this lignocellulosic sugar platform.

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Synthesis of temperature and pH responsive lignin-grafted sulfobetaine for efficiently recycling cellulase

Pang²,

Li³

et al. 2023

Bioresource Technology

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Effect of cellulase on the UCST behavior of sulfobetaine zwitterionic surfactants and the cellulase recovery mechanism

Abstract: That recovering and recycling cellulase at room temperature by adding recyclable thermoresponsive zwitterionic surfactant is more significant for reducing the cost of the production of cellulosic ethanol. Sulfobetaines can...

Cited by 5 publications

References 21 publications

Synthesis of Recyclable UCST-Type Copolymer PPSP and Its Application in Enhancing Lignocellulosic Enzymatic Hydrolysis and Recycling Cellulase

Synthesis of Recyclable UCST-Type Copolymer PPSP and Its Application in Enhancing Lignocellulosic Enzymatic Hydrolysis and Recycling Cellulase

Rational design of temperature‐responsive binary polymers containing sulfobetaine and quaternary ammonium fragments for application in lignocellulosic hydrolysis

Synthesis of temperature and pH responsive lignin-grafted sulfobetaine for efficiently recycling cellulase

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