Effect of Centrifugation on the Storage Properties of Platelets

Solberg, C.; Moen, P.; Little, C.

doi:10.1159/000461846

Cited by 5 publications

(6 citation statements)

References 13 publications

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“…In our study, PCs were plasma-depleted by centrifugation at 3500g for four minutes. A short centrifugation for 2.25 minutes at 3270g yielded platelets with better storage properties than with longer centrifugation times (4.5 minutes at 2200g) (21). Exposure of platelets to physical stimuli such as centrifugation has been reported to diminish cyclic adenosine monophosphate levels (22).…”

Section: Discussionmentioning

confidence: 99%

The Functional Integrity of Platelets in Volume-Reduced Platelet Concentrates

Schoenfeld¹,

Muhm²,

Doepfmer³

et al. 2005

Anesthesia & Analgesia

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Premature and low-birth-weight infants usually require small-volume platelet transfusions to treat thrombocytopenia. Also, infants undergoing open-heart surgery with extracorporeal circulation and with compromised cardiac function are at risk for excessive intravascular volume. The small-volume platelet substitution can be achieved by dispensing an aliquot from the unit of a standard single-donor platelet concentrate (PC). Alternatively, there is an indication for volume reduction of PCs to maximize the number of platelets transfused in the smallest possible volume. We determined the spontaneous and induced activation of platelets before and after volume reduction in 20 consecutive single-donor-apheresis PCs. After a mean storage time of 2 days, the PCs were plasma-depleted by centrifugation. Spontaneous, adenosine diphosphate (ADP)-induced, and collagen-induced activation were determined by flow cytometry. Furthermore, ADP- and collagen-induced aggregation were measured. A total of 33.8% of platelets in standard PCs were activated spontaneously. Volume reduction of PCs led to a mild but significant increase of spontaneous activation of platelets (43.2%). Additionally, volume reduction resulted in an impaired ADP-induced aggregability of platelets, whereas collagen induction was unaffected. Transfusion of volume-reduced PCs is an effective alternative to use of standard PCs in patients at frequent risk for excessive intravascular volume, because equal volumes increase the platelet count twice as effectively.

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Section: Discussionmentioning

confidence: 99%

The Functional Integrity of Platelets in Volume-Reduced Platelet Concentrates

Schoenfeld¹,

Muhm²,

Doepfmer³

et al. 2005

Anesthesia & Analgesia

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“…The resulting lactate accumulation requires sufficient buffer capacity within the storage medium, because the maintenance of an adequate pH is necessary to prevent severe structural alterations and the release reaction which occur below pH 6 [73,84]. During the past few years, the use of suitable buffer systems and of storage bag materials with good gas permeability has led to only minimal changes or even steadiness of the pH [13,19,25,39,47,84,85], particularly in BC-PC [57]. The increasing consumption of glucose during storage is accompanied by a drop in the energy charge of the platelets [13,19,35,80], but no definitive data are yet available on the resulting deficiencies regarding functional in vitro tests, in vivo survival, and hemostatic efficacy [70].…”

Section: Metabolismmentioning

confidence: 99%

The storage lesion of platelets: ultrastructural and functional aspects

Klinger

1996

Annals of Hematology

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When prepared and stored as concentrates, platelets undergo a lot of structural, biochemical and functional alterations that lead to an impaired function after transfusion. Besides signs of activation like disc-to-sphere transformation, extension of pseudopodes and loss of storage granules, platelets may display a swollen open canalicular system and changes in the structure of their alpha-granules. These partly reversible morphological alterations correspond to a deterioration of basic metabolic parameters and a decrease in the reactivity of stored platelets to weak agonists. All these changes occur to a very different degree depending on the methods of preparation and storage. With the introduction of acetate-containing additive solutions, the storage conditions could be greatly improved, and platelets from pooled buffy coats and stored in an acetate-containing medium with at least 20% autologous plasma show the best structural integrity over 8 days of storage.

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“…The basic centrifugation protocol has been described elsewhere. 8 We used two different protocols, of 7 or 20 minutes, for the second centrifugation. After the removal of the supernatant plasma, the platelet button was undisturbed for approximately 90 minutes before resuspension.…”

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confidence: 99%

Adenylate kinase as a marker for platelet lysis

Gulliksson¹

1990

Transfusion

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The level of extracellular lactate dehydrogenase (LDH) activity is often used as an indicator for the leakage of cytosolic platelet contents. The objective of this investigation was to study the possibility of using a different enzyme, adenylate kinase (AK), as a substitute for LDH. A bioluminescent assay was used, and three different platelet storage containers were studied. In a comparative study of AK and LDH activities during platelet storage, similar results were obtained, but some differences were also observed. Because more AK (in percentages) than LDH is contained in platelets, the initial levels of AK are lower than those of LDH. The release of AK activity from damaged platelets is delayed or is somewhat lower than that of LDH. The amount of AK activity released per platelet is correlated with the volume of the platelet units. A smaller volume entails increased AK activity. This observation applies to all storage conditions investigated, and the results in one of the containers indicate that this is also true for LDH. The results of the investigation demonstrate that similar information is obtained with LDH and AK. AK can be used as an alternative to LDH for studies of lytic processes during platelet storage.

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Effect of Centrifugation on the Storage Properties of Platelets

Cited by 5 publications

References 13 publications

The Functional Integrity of Platelets in Volume-Reduced Platelet Concentrates

The Functional Integrity of Platelets in Volume-Reduced Platelet Concentrates

The storage lesion of platelets: ultrastructural and functional aspects

Adenylate kinase as a marker for platelet lysis

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