Effect of Cerium Oxide Nanoparticles on Sepsis Induced Mortality and NF-κB Signaling in Cultured Macrophages

Selvaraj, Vellaisamy; Manne, Nandini D.P.K.; Arvapalli, Ravikumar; Rice, Kevin M.; Nandyala, Geeta; Fankenhanel, Erin; Blough, Eric R.

doi:10.2217/nnm.14.205

Cited by 64 publications

(38 citation statements)

References 39 publications

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“…*vs control, # vs cI: P,0.05 by two-way repeated measures aNOVa. diminished systemic inflammation 15 with the latter occurring most likely due to diminished Kupffer cell activation. 21 In this study, we show that CeO 2 nanoparticle treatment attenuates the diaphragm dysfunction induced by the septic condition.…”

Section: Discussionmentioning

confidence: 99%

“…15 Whether this increase in survivability following CeO 2 nanoparticle intervention also associated with improvements of diaphragm function is currently unclear. On the basis of previous data demonstrating that rodent diaphragm is particularly susceptible to elevations in oxidative stress and circulating cytokines, [16][17][18] we hypothesized that septic animals that had been treated with CeO 2 nanoparticles would exhibit improved diaphragmatic function compared with that observed in the untreated counterparts.…”

Section: Asano Et Almentioning

confidence: 99%

“…This dose was chosen based on our preliminary data from pilot studies examining animal mortality and previously published data. 15,21 The sham control and the CI groups received vehicle (200 µL of sterile distilled water). For the biochemical assessments, animals were sacrificed at 3 or 18 hours while functional assessments of diaphragm contractility were performed at 18 hours.…”

Section: Ceo 2 Nanoparticle Treatmentmentioning

confidence: 99%

“…These time points were selected based on previous data suggesting that the 3-hour time point appears to represent the initial systemic inflammatory response syndrome phase while the 18-hour time point coincides with the development of multiple organ dysfunction. 15 …”

Section: Ceo 2 Nanoparticle Treatmentmentioning

confidence: 99%

“…15 SEM and TEM analyses suggested that the particles were likely spherical in shape and ∼15-20 nm in diameter ( Figure 1A and B). ceO 2 nanoparticle treatment improves diaphragmatic function in the septic rat There were significant differences in pre-/post-body weight and serum hematocrit levels for the CI and CI+CeO 2 groups but not for the sham and CeO 2 -only treated animals at 18 hours after the surgery ( Table 1).…”

Section: Ceo 2 Nanoparticle Characterizationmentioning

confidence: 99%

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Cerium oxide nanoparticle treatment ameliorates peritonitis-induced diaphragm dysfunction

Asano¹,

Arvapalli²,

Manne³

et al. 2015

IJN

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The severe inflammation observed during sepsis is thought to cause diaphragm dysfunction, which is associated with poor patient prognosis. Cerium oxide (CeO 2 ) nanoparticles have been posited to exhibit anti-inflammatory and antioxidative activities suggesting that these particles may be of potential use for the treatment of inflammatory disorders. To investigate this possibility, Sprague Dawley rats were randomly assigned to the following groups: sham control, CeO 2 nanoparticle treatment only (0.5 mg/kg iv), sepsis, and sepsis+CeO 2 nanoparticles. Sepsis was induced by the introduction of cecal material (600 mg/kg) directly into the peritoneal cavity. Nanoparticle treatment decreased sepsis-associated impairments in diaphragmatic contractile ( P o ) function (sham: 25.6±1.6 N/cm 2 vs CeO 2 : 23.4±0.8 N/cm 2 vs Sep: 15.9±1.0 N/cm 2 vs Sep+CeO 2 : 20.0±1.0 N/cm 2 , P <0.05). These improvements in diaphragm contractile function were accompanied by a normalization of protein translation signaling (Akt, FOXO-1, and 4EBP1), diminished proteolysis (caspase 8 and ubiquitin levels), and decreased inflammatory signaling (Stat3 and iNOS). Histological analysis suggested that nanoparticle treatment was associated with diminished sarcolemma damage and diminished inflammatory cell infiltration. These data indicate CeO 2 nanoparticles may improve diaphragmatic function in the septic laboratory rat.

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Section: Discussionmentioning

confidence: 99%

Section: Asano Et Almentioning

confidence: 99%

Section: Ceo 2 Nanoparticle Treatmentmentioning

confidence: 99%

Section: Ceo 2 Nanoparticle Treatmentmentioning

confidence: 99%

Section: Ceo 2 Nanoparticle Characterizationmentioning

confidence: 99%

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Cerium oxide nanoparticle treatment ameliorates peritonitis-induced diaphragm dysfunction

Asano¹,

Arvapalli²,

Manne³

et al. 2015

IJN

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Ce₁₂V₆ Clusters with Multi‐Enzymatic Activities for Sepsis Treatment

Liu,

Sun,

Qiao

et al. 2024

Adv Healthcare Materials

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Artificial enzymes, especially nanozymes, have attracted wide attention due to their controlled catalytic activity, selectivity, and stability. The rising Cerium‐based nanozymes exhibit unique SOD‐like activity, and Vanadium‐based nanozymes always hold excellent GPx‐like activity. However, most inflammatory diseases involve polymerase biocatalytic processes that require multi‐enzyme activities. The nanocomposite can fulfill multi‐enzymatic activity simultaneously, but large nanoparticles (>10 nm) cannot be excreted rapidly, leading to biosafety challenges. Herein, atomically precise Ce12V6 clusters with a size of 2.19 nm are constructed. The Ce12V6 clusters show excellent glutathione peroxidase (GPx) ‐like activity with a significantly lower Michaelis‐Menten constant (Km, 0.0125 mM versus 0.03 mM of natural counterpart) and good activities mimic superoxide dismutase (SOD) and peroxidase (POD). The Ce12V6 clusters exhibit the ability to scavenge the ROS including O2·− and H2O2 via the cascade reactions of multi‐enzymatic activities. Further, the Ce12V6 clusters modulate the proinflammatory cytokines including tumor necrosis factor‐alpha (TNF‐α), interleukin‐6 (IL‐6), and interleukin‐1β (IL‐1β) and consequently rescue the multi‐organ failure in the lipopolysaccharide (LPS)‐induced sepsis mouse model. With excellent biocompatibility, the Ce12V6 clusters show promise in the treatment of sepsis.

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Large‐Scale Synthesis and Medical Applications of Uniform‐Sized Metal Oxide Nanoparticles

et al. 2018

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Thanks to recent advances in the synthesis of high-quality inorganic nanoparticles, more and more types of nanoparticles are becoming available for medical applications. Especially, metal oxide nanoparticles have drawn much attention due to their unique physicochemical properties and relatively inexpensive production costs. To further promote the development and clinical translation of these nanoparticle-based agents, however, it is highly desirable to reduce unwanted interbatch variations of the nanoparticles because characterizing and refining each batch are costly, take a lot of effort, and, thus, are not productive. Large-scale synthesis is a straightforward and economic pathway to minimize this issue. Here, the recent achievements in the large-scale synthesis of uniform-sized metal oxide nanoparticles and their biomedical applications are summarized, with a focus on nanoparticles of transition metal oxides and lanthanide oxides, and clarifying the underlying mechanism for the synthesis of uniform-sized nanoparticles. Surface modification steps to endow hydrophobic nanoparticles with water dispersibility and biocompatibility are also briefly described. Finally, various medical applications of metal oxide nanoparticles, such as bioimaging, drug delivery, and therapy, are presented.

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Effect of Cerium Oxide Nanoparticles on Sepsis Induced Mortality and NF-κB Signaling in Cultured Macrophages

Abstract: The findings of this study indicate that CeO2 nanoparticles may be useful for the treatment of sepsis.

Cited by 64 publications

References 39 publications

Cerium oxide nanoparticle treatment ameliorates peritonitis-induced diaphragm dysfunction

Cerium oxide nanoparticle treatment ameliorates peritonitis-induced diaphragm dysfunction

Ce₁₂V₆ Clusters with Multi‐Enzymatic Activities for Sepsis Treatment

Large‐Scale Synthesis and Medical Applications of Uniform‐Sized Metal Oxide Nanoparticles

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Effect of Cerium Oxide Nanoparticles on Sepsis Induced Mortality and NF-κB Signaling in Cultured Macrophages

Abstract: The findings of this study indicate that CeO2 nanoparticles may be useful for the treatment of sepsis.

Cited by 64 publications

References 39 publications

Cerium oxide nanoparticle treatment ameliorates peritonitis-induced diaphragm dysfunction

Cerium oxide nanoparticle treatment ameliorates peritonitis-induced diaphragm dysfunction

Ce12V6 Clusters with Multi‐Enzymatic Activities for Sepsis Treatment

Large‐Scale Synthesis and Medical Applications of Uniform‐Sized Metal Oxide Nanoparticles

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Product

Resources

About

Ce₁₂V₆ Clusters with Multi‐Enzymatic Activities for Sepsis Treatment