During a rapid compression machine (RCM) experiment, the temperature field that develops is expected to strongly depend on the initial temperature of the gas and, thus, the wall boundary temperatures. Two computational fluid dynamics (CFD) studies, one with buoyancy and one without buoyancy, have been conducted to investigate the role of nonuniform boundary temperatures and buoyancy as a potential cause for the top-to-bottom flame propagation behavior observed in previous ethanol autoignition experiments using a RCM. The effect of these boundary conditions and buoyancy on the initial temperature field development and how it evolves during and after compression has been investigated for ethanol autoignition using a creviced piston. Simulations with and without buoyancy showed that a unique temperature gradient forms in each case over the piston surface due to the nonuniformities present in the initial temperature fields. Finally, the simulations demonstrated the effect of these unique nonuniformities on ethanol autoignition behavior. It was determined through this work that simulations with buoyancy considered closely reproduced the features of the ethanol autoignition that were observed in the experiments.