Effect of Chemical Modification of a Histidine and a Lysine Residue of Pea Seed Nucleoside Diphosphate Kinase

Abstract

Chemical m o d i f i c a t i o n o f a h i s t i d i n e and l y s i n e r e s i d u e i n a c t i v a t e s pea seed n u c l e o s i d e diphosphate k i n a s e (NDP k i n a s e ) . r e a c t i v e l y s i n e r e s i d u e , a t t h e a c t i v e s i t e o f pea seed NDP kinase, i n a d d it i o n t o t h e h i s t i d i n e r e s i d u e phosphorylated b y t h e s u b s t r a t e ATP as a con-sequence o f t h e enzyme r e a c t i o n . The presence o f a r e a c t i v e l y s i n e a t t h e