Effect of chemical structure of hydrogels on the adhesion and phenotypic characteristics of human monocytes such as expression of galectins and other carbohydrate-binding sites

Smetana, Karel; Lukáš, J.; Palečková, Věra; Bartůňková, Jiřina; Liu, Fu‐Tong; Vacı́k, J.; Gabius, Hans‐Joachim

doi:10.1016/s0142-9612(97)00037-9

Cited by 33 publications

(14 citation statements)

References 28 publications

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“…Cell adhesion can be reduced if the surface is modified with an antiadhesive coating. Two groups of chemicals, hydrophobic silanes [12], and hydrophilic polymers (hydrogels) [13,14], have been most often used in biological studies to reduce cell adhesion to the surface. Here, we studied the feasibility of glass and polystyrene surfaces modified with representatives of these two groups to be used as cell supports for cell injection into the capillary.…”

Section: Untreated Surfacesmentioning

confidence: 99%

“…Coating with hydrogels is known to prevent strong protein and cell adhesion to the surface [13,14]. The surface can be modified by creating a physical layer of a hydrogel or by covalent binding of a hydrogel to the surface material.…”

Section: Hydrophilic Coatingmentioning

confidence: 99%

“…Adhesion can be reduced by changing the physical-chemical properties of the surface, such as electrical charge, hydrophobicity, and chemical structure. A number of chemicals, mainly of two groups, hydrophobic silanes [12] and hydrophilic polymers (hydrogels) [13,14], have been reported to reduce cell adhesion to the surface. Here we evaluated several substances as potential modifiers of glass and polystyrene surfaces to be used as cell support materials for cell injection into the capillary.…”

Section: Introductionmentioning

confidence: 99%

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Single-cell analysis using capillary electrophoresis: Influence of surface support properties on cell injection into the capillary

Krylov

Dovic̀hi

2000

Electrophoresis

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Section: Untreated Surfacesmentioning

confidence: 99%

Section: Hydrophilic Coatingmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Single-cell analysis using capillary electrophoresis: Influence of surface support properties on cell injection into the capillary

Krylov

Dovic̀hi

2000

Electrophoresis

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“…Since it is the biomaterial surface that first comes into contact with the living tissue when the biomaterial is planted in the body, the initial response of the body to the biomaterial depends on its surface properties. Surface properties that can influence biocompatibility include surface charge, surface topography, etc [24][25][26][27][28][29]. To examine the cell-matrix interactions, HaCat cells were cultivated on the surface of the composite membranes, their morphology and distribution were observed and photographed by a phase contrast microscope on day 1, 3, 5, 7 d. Figure 6 and Figure 7 show the morphology of HaCat cell on the composite membrane on day 1 and day 7, respectively.…”

Section: Morphology Of Hacat Cell Adhered On the Surface Of Compositementioning

confidence: 99%

Preparation, properties, and cell attachment/growth behavior of chitosan/acellular derm matrix composite materials

Lü¹,

Li²,

Zhang³

et al. 2011

JBNB

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Composite membranes and sponge scaffolds consisting chitosan (CS) and acellular derm matrix (ADM) in six ratios were prepared by solvent evaporation technique and freeze-drying method, respectively. The composite materials were characterized by water contact angle measurement, scanning electron microscopy (SEM), water absorption and HaCat cells compatibility. The SEM result showed that CS/ADM three-dimensional (3D) micro-porous structures were successfully produced. The water absorption value of all scaffolds was over 18 times of its initial weight, which is high enough for skin regeneration scaffold, but there were no significant differences of water absorption ratio between deionized water and PBS solution for same scaffold (P > 0.05). HaCat cells were distributed uniformly on the surfaces of membrane 4 -6, and an almost confluent monolayer was formed on membrane 6 on the fifth day, whereas cells maintained round and spherical in shape on the surface of membrane 1. The results showed that the cell compatibility of pure CS membrane needed to be improved, and addition of ADM realized this purpose. The results of compatibility of HaCat cells on scaffolds showed that the cell proliferated well on the scaffolds 3 and 4. In our study, the cell's attachment and growth on the composite membranes was mainly determined by the content of the membrane, whereas the cell's attachment and growth in the scaffolds was determined by both the content and structure of the scaffolds.

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“…The cell adhesion characteristics of p(HEMA) have been improved by the incorporation of collagen14 and by the addition of hydrophobic caprolactone 15. Smetana et al 7 found that monocyte adhesion was much greater onto co-polymers of p(HEMA) and positively charged 2-(dimethylamino)ethyl methacrylate (DEM) (10 mol%) when compared with that onto p(HEMA) or a co-polymer of p(HEMA) and the negatively charged salt of methacrylic acid (MA) (3 mol%). Bergethon et al 10 had previously shown that the incorporation of either of the ionisable groups MA or DEM (0.1% vol) produced fibroblast spreading onto p(HEMA) hydrogels.…”

mentioning

confidence: 99%

Novel materials to enhance keratoprosthesis integration

Sandeman

2000

British Journal of Ophthalmology

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Background-The successful integration of keratoprostheses (KPros) within the cornea depends in part on peripheral host keratocyte adhesion to anchor the implant in place and prevent epithelial downgrowth. The following study incorporated diVerent acrylate co-monomers with poly(hydroxyethyl methacrylate) (p(HEMA)) and measured the suitability of these materials as potential skirt materials in terms of their ability to enhance keratocyte adhesion to p(HEMA). Methods-p(HEMA) hydrogels incorporating varying amounts of the acrylate co-monomers methacrylic acid (MA), 2-(dimethylamino)ethyl methacrylate (DEM), or phenoxyethyl methacrylate (PEM) were formed by free radical polymerisation. Keratocytes were seeded onto discs of each material and incubated at 37°C for 72 hours. Assays for viable cell adhesion were carried out. A viability/ cytotoxicity assay using solutions of calcein-AM (0.5 mM) and ethidium homodimer-1 (EthD-1) (0.5 µM) were used to measure viable and non-viable cell adhesion, respectively. An ATP assay was also used to quantify cell adhesion in terms of the amount of ATP present following lysis of adherent cells. Results-The viability/cytotoxicity assays indicated that the incorporation of 15 mol% of the co-monomer PEM or of 20 mol% DEM increased cell adhesion to p(HEMA) by at least four times. The ATP assays confirmed the results for PEM but absorption of ATP to the DEM containing hydrogel indicated that the assay was not a suitable measure of cell adhesion to this material. Conclusions-Theproperties of p(HEMA) may be moderated to enhance keratocyte adhesion by the incorporation of PEM or DEM suggesting that these may be suitable materials for use in the further development of a novel KPro skirt material. (Br J Ophthalmol 2000;84:640-644)

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Effect of chemical structure of hydrogels on the adhesion and phenotypic characteristics of human monocytes such as expression of galectins and other carbohydrate-binding sites

Cited by 33 publications

References 28 publications

Single-cell analysis using capillary electrophoresis: Influence of surface support properties on cell injection into the capillary

Single-cell analysis using capillary electrophoresis: Influence of surface support properties on cell injection into the capillary

Preparation, properties, and cell attachment/growth behavior of chitosan/acellular derm matrix composite materials

Novel materials to enhance keratoprosthesis integration

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