Effect of chemically synthesis compared to biosynthesized ZnO nanoparticles using aqueous extract of C. halicacabum and their antibacterial activity

Nithya, K.; Kalyanasundharam, S.

doi:10.1016/j.onano.2018.10.001

Cited by 82 publications

(42 citation statements)

References 21 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…FTIR spectrum wavenumber of ZnO NPs (Figure 2d) confirms the presence of polyphenol, flavonoids, carbonyl, and aliphatic amine group of the plant extract are documented to act as both reducing and capping [65]. A sharp peak in the lower energy region was found at 450 cm −1 confirming Zn-O bond bending vibration as metal-oxygen are reported in the range of 400 to 600 nm [66]. Therefore, FTIR assessed for C. verrucosa mediated ZnO NPs ( Figure 2d) corroborates with the result of FTIR spectrum of leaf extract, which shows that the organic compounds (phenols and flavonoid) of C. verrucosa still retain their original properties after calcination during ZnO synthesis and hence reduced the synthesized ZnO NPs.…”

Section: Zno Nps Synthesis and Its Characterizationmentioning

confidence: 59%

“…Zeta potential povides the zeta value which shows surface charge and stability of ZnO NPs (prepared using C. verrucosa leaf extract) was −21 mV ( Figure 4 a) demonstrating its stability. The hydroxyl group of C. verrucosa leaf extract is responsible for reduction of metal ion due to its strong binding affinity so that they can act as capping agent to avoid nanoparticle aggregation assuring its negative charge [ 38 ] and are in agreement with biosynthesized ZnO NPs from S. album leaf extract [ 67 ] and using C. halicacabum leaf extract [ 66 ]. TGA is used to assess the thermal stability of the synthesized NPs (20 mg) by subjecting the NPs to a temperature range of 0–1000 °C ( Figure 4 b) The reduction in weight measured at ~120 °C corresponds to the desertion of humidity.…”

Section: Resultsmentioning

confidence: 95%

See 1 more Smart Citation

Crotalaria verrucosa Leaf Extract Mediated Synthesis of Zinc Oxide Nanoparticles: Assessment of Antimicrobial and Anticancer Activity

et al. 2020

View full text Add to dashboard Cite

In this work, we present an ecofriendly, non-hazardous, green synthesis of zinc oxide nanoparticles (ZnO NPs) by leaf extract of Crotalaria verrucosa (C. verrucosa). Total phenolic content, total flavonoid and total protein contents of C. verrucosa were determined. Further, synthesized ZnO NPs was characterized by UV–visible spectroscopy (UV-vis), X-ray diffractometer (XRD), Fourier transform infra-red (FTIR) Spectra, transmission electron microscope (TEM), and Dynamic light scattering (DLS) analysis. UV-vis shows peak at 375 nm which is unique to ZnO NPs. XRD analysis demonstrates the hexagonal phase structures of ZnO NPs. FTIR spectra demonstrates the molecules and bondings associated with the synthesized ZnO NPs and assures the role of phytochemical compounds of C. verrucosa in reduction and capping of ZnO NPs. TEM image exhibits that the prepared ZnO NPs is hexagonal shaped and in size ranged between 16 to 38 nm which is confirmed by DLS. Thermo-gravimetric analysis (TGA) was performed to determine the thermal stability of biosynthesized nanoparticles during calcination. The prepared ZnO NPs showed significant antibacterial potentiality against Gram-positive (S. aureus) and Gram-negative (Proteus vulgaris, Klebsiella pneumoniae, and Escherichia coli) pathogenic bacteria and SEM image shows the generalized mechanism of action in bacterial cell after NPs internalization. In addition, NPs are also found to be effective against the studied cancer cell lines for which cytotoxicity was assessed using MTT assay and results demonstrate highest growth of inhibition at the concentration of 100 µg/mL with IC50 value at 7.07 µg/mL for HeLa and 6.30 µg/mL for DU145 cell lines, in contrast to positive control (C. verrucosa leaf extract) with IC50 of 22.30 µg/mL on HeLa cells and 15.72 µg/mL on DU145 cells. Also, DAPI staining was performed in order to determine the effect on nuclear material due to ZnO NPs treatment in the studied cell lines taking leaf extract as positive control and untreated negative control for comparison. Cell migration assay was evaluated to determine the direct influence of NPs on metastasis that is potential suppression capacity of NPs to tumor cell migration. Outcome of the synthesized ZnO NPs using C. verrucosa shows antimicrobial activity against studied microbes, also cytotoxicity, apoptotic mediated DNA damage and antiproliferative potentiality in the studied carcinoma cells and hence, can be further used in biomedical, pharmaceutical and food processing industries as an effective antimicrobial and anti-cancerous agent.

show abstract

Section: Zno Nps Synthesis and Its Characterizationmentioning

confidence: 59%

Section: Resultsmentioning

confidence: 95%

Crotalaria verrucosa Leaf Extract Mediated Synthesis of Zinc Oxide Nanoparticles: Assessment of Antimicrobial and Anticancer Activity

et al. 2020

View full text Add to dashboard Cite

show abstract

“…Furthermore, we compared our results with a literature report on chemically synthesized ZnONPs to account for the significance of as-obtained ZnONPs using an aqueous extract of C. auriculata leaves. The results demonstrate that the as-obtained ZnONPs showed a greater antibacterial potency as compared to the commercially available ZnONPs [31].…”

Section: Bactericidal Activitymentioning

confidence: 80%

“…The XRD pattern reveals the crystalline nature of as-obtained ZnONPs. The significant diffraction peaks observed at angles (2θ) 31 (201), and (004) planes, respectively. In addition, a less intense peak observed at 2θ = 42.3 • reflects the amorphous phase of the biological functional groups from the organic components of the Cassia auriculata leaf extract [27].…”

Section: Absorption and Emission Spectral Studiesmentioning

confidence: 99%

Tumoricidal and Bactericidal Properties of ZnONPs Synthesized Using Cassia auriculata Leaf Extract

Prasad

Ansari

et al. 2020

Biomolecules

View full text Add to dashboard Cite

In this work, we aimed to synthesize zinc oxide nanoparticles (ZnONPs) using an aqueous extract of Cassia auriculata leaves (CAE) at room temperature without the provision of additional surfactants or capping agents. The formation of as-obtained ZnONPs was analyzed by UV–visible (ultraviolet) absorption and emission spectroscopy, X-ray photoemission spectroscopy (XPS), X-ray diffraction analysis (XRD), energy dispersive X-ray diffraction (EDX), thermogravimetric analysis/differential thermal analysis (TGA-DTA), scanning electron microscopy (SEM), transmission electron microscopy (TEM), high-resolution transmission electron microscopy (HRTEM), and selected area electron diffraction (SAED). The XRD results reflect the wurtzite structure of as-prepared ZnONPs, which produced diffraction patterns showing hexagonal phases. The SEM images indicate that the morphology of as-prepared ZnONPs is composed of hexagonal nanostructures with an average diameter of 20 nm. The HR-TEM result shows that the inter-planar distance between two lattice fringes is 0.260 nm, which coincides with the distance between the adjacent (d-spacing) of the (002) lattice plane of ZnO. The fluorescence emission spectrum of ZnONPs dispersed in ethanol shows an emission maximum at 569 nm, revealing the semiconductor nature of ZnO. As-obtained ZnONPs enhanced the tumoricidal property of CAE in MCF-7 breast cancer cells without significant inhibition of normal human breast cells, MCF-12A. Furthermore, we have studied the antibacterial effects of ZnONPs, which showed direct cell surface contact, resulting in the disturbance of bacterial cell integrity.

show abstract

“…According to Ma et al [ 7 ], the simplest route is an acid–base precipitation method which was widely used to obtain ZnO nanoparticles for either biological or coating application. An aqueous precipitation process was employed using precursors, such as zinc acetate (Zn(CH 3 COO) 2 ) [ 8 ], zinc nitrate (Zn(NO 3 ) 2 ) [ 9 ] or zinc sulfate (ZnSO 4 ) [ 10 ], and an alkaline aqueous solution, such as sodium hydroxide (NaOH), are usually prepared in deionized/distilled water.…”

Section: Introductionmentioning

confidence: 99%

Effect of Synthesis Temperature on the Size of ZnO Nanoparticles Derived from Pineapple Peel Extract and Antibacterial Activity of ZnO–Starch Nanocomposite Films

et al. 2020

View full text Add to dashboard Cite

This research investigated the effect of synthesis temperature on the size and shape of zinc oxide (ZnO) nanoparticles (NPs) synthesized using pineapple peel waste and antibacterial activity of ZnO NPs in starch films. Zinc oxide NPs synthesized at different temperatures were characterized by Fourier transform infrared spectroscopy, X-ray diffraction analysis, field-emission scanning electron microscopy, energy-dispersive X-ray spectroscopy, and transmission electron microscopy. Micrographs of ZnO NPs synthesized at 28 and 60 °C showed that synthesis temperature affected the sizes and shapes of ZnO NPs. The non-heated (28 °C) condition resulted in NPs with diameters in the range of 8–45 nm with a mixture of spherical and rod shapes, whereas the heated (60 °C) condition led to NPs with diameters in the range of 73–123 nm with flower rod shapes. The ZnO–starch nanocomposite films incorporated with 1, 3, and 5 wt.% ZnO NPs were prepared via a film casting method. The antibacterial activity of the films against Gram-positive and Gram-negative bacteria was investigated using the disc diffusion method. The results showed an increase in the inhibition zone for Gram-positive bacteria, particularly Bacillus subtilis, when the concentration of ZnO NPs incorporated in the film was increased from 1 to 5 wt.%.

show abstract

Effect of chemically synthesis compared to biosynthesized ZnO nanoparticles using aqueous extract of C. halicacabum and their antibacterial activity

Cited by 82 publications

References 21 publications

Crotalaria verrucosa Leaf Extract Mediated Synthesis of Zinc Oxide Nanoparticles: Assessment of Antimicrobial and Anticancer Activity

Crotalaria verrucosa Leaf Extract Mediated Synthesis of Zinc Oxide Nanoparticles: Assessment of Antimicrobial and Anticancer Activity

Tumoricidal and Bactericidal Properties of ZnONPs Synthesized Using Cassia auriculata Leaf Extract

Effect of Synthesis Temperature on the Size of ZnO Nanoparticles Derived from Pineapple Peel Extract and Antibacterial Activity of ZnO–Starch Nanocomposite Films

Contact Info

Product

Resources

About