Effect of chitosan on the enamel demineralization process <i>in vitro</i>: an enamel solubility test

Surija, I.; Gunawan, Harun Asjiq; Amir, Lisa Rinanda

doi:10.1088/1742-6596/1073/5/052005

Cited by 3 publications

(1 citation statement)

References 7 publications

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“…3,38,41 Chitosan disrupts the H + ions of the acid solution, inhibiting the demineralization process and increasing the pH of the solution. 42 The chitosan-based layer formed by the reaction of these biopolymer molecules with the enamel surface leads to coverage of at least a few nanometers 43 of a ubiquitous chitosan layer. 44 The variation in the sample surface may explain the high standard deviation in volume loss found in this study.…”

Section: Discussionmentioning

confidence: 99%

Effect of an experimental chitosan/casein gel on demineralized enamel under a cariogenic challenge

et al. 2022

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Background. Dental caries is considered one of the most common oral health diseases.Objectives. The aim of the study was to evaluate the effects of an experimental chitosan/casein gel on enamel demineralization/remineralization in an environment with a high cariogenic challenge. Material and methods.Thirty-six specimens of bovine enamel (4 mm × 3 mm × 2 mm) were ground flat and polished. Then, the specimens were immersed in acetate buffer for 43 h with half of the surface protected (serving as control) and the other half exposed. All demineralized surfaces were randomly assigned into 3 groups (n = 12 per group) according to the type of treatment (G1 -control, G2 -1.5% chitosan gel with 1.5% casein, and G3 -1.5% chitosan gel without casein), and the corresponding treatment was applied once a week for 3 weeks. The specimens were also subjected to pH cycles of demineralization/ remineralization and the treatments were performed 3 times at 7-day intervals for a total of 21 days. Surface images were obtained for the analysis of initial roughness and, after the cariogenic challenge, new images were obtained to evaluate the final roughness, volume loss and wear profile using laser confocal microscopy. After the analyses, the specimens were cut and the depth of demineralization was measured. The data were analyzed using the Kruskal-Wallis analysis of variance (ANOVA) and the Tukey's test.Results. While the chitosan gel with casein showed a similar loss to the control group (p > 0.05), both gels resulted in similar volume loss (p > 0.05). There were no statistical differences regarding the wear profile, surface roughness and depth of demineralization between the groups (p > 0.05).Conclusions. The chitosan gel reduced volume loss of the demineralized enamel without significantly impacting the surface smoothness.

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Section: Discussionmentioning

confidence: 99%

Effect of an experimental chitosan/casein gel on demineralized enamel under a cariogenic challenge

et al. 2022

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Silver Nanoparticles–Chitosan Nanocomposites as Protective Coatings for Dental Remineralization Treatment: An In Vitro Study

de Rodríguez,

de González,

Castañeda Monroy

et al. 2025

Coatings

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Research with nanoparticles for the treatment and prevention of dental caries is of special interest given the high prevalence of the disease worldwide. Several studies support the use of nanoparticles associated with materials given their antimicrobial properties and potential demineralization reduction. This study aimed to evaluate the impact of the application of silver nanoparticles (AgNPs) and chitosan gel in combination with commercial fluoride varnish on the remineralization of dental enamel. Ninety-six tooth blocks were macroscopically evaluated via stereomicroscopy, ICDAS II, and laser fluorescence. Enamel blocks were subjected to artificial demineralization and divided into four exposure groups (24, 48, 120, and 168 h), and five different remineralizing agents were applied, namely, FV (fluoride varnish), FV + CG (fluoride varnish + chitosan gel), FV + AgNPs (fluoride varnish + AgNPs), FV + AgNPs + CG (fluoride varnish + AgNPs + chitosan gel), and AgNPs + CG (AgNPs + chitosan gel). Enamel surface changes were evaluated via laser fluorescence, X-ray diffraction, scanning electron microscopy, and energy-dispersive X-ray spectroscopy. Laser fluorescence results obtained from demineralized blocks and subsequently exposed to remineralizing treatment indicate significant differences. After exposure to remineralizing agents, hydroxyapatite and modified apatite phases were identified mainly in the samples treated with FV + AgNPs + CG in the groups exposed for 24, 48, and 120 h. The FV + AgNPs + CG and AgNPs + CG indicate good performance in terms of the Ca/P ratio in in vitro demineralization compared to the group treated with fluorine varnish.

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Applicability of a Natural Nano-derivative as a Mouth Rinse on Salivary pH and S. mutans Count: An Ex Vivo Study

Prathigudupu,

Gavarraju,

Kallam

et al. 2023

World Journal of Dentistry

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Effect of chitosan on the enamel demineralization process in vitro: an enamel solubility test

Cited by 3 publications

References 7 publications

Effect of an experimental chitosan/casein gel on demineralized enamel under a cariogenic challenge

Effect of an experimental chitosan/casein gel on demineralized enamel under a cariogenic challenge

Silver Nanoparticles–Chitosan Nanocomposites as Protective Coatings for Dental Remineralization Treatment: An In Vitro Study

Applicability of a Natural Nano-derivative as a Mouth Rinse on Salivary pH and S. mutans Count: An Ex Vivo Study

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