Effect of Chloramphenicol on Light Dependent Development of Seedlings of Phaseolus vulgaris var. Black Valentine, With Particular Reference to Development of Photosynthetic Activity

Margulies, Maurice M.

doi:10.1104/pp.37.4.473

Cited by 62 publications

(37 citation statements)

References 14 publications

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“…As shown in table I, for the 10 to 12 day-old leaves used in these experiments, after the photoconversion of the protochlorophyllide present before illumination, a slow increase in chlorophyll content occurred. After 3 hours in the light, the level of chlorophyll was approximately 10 times greater than that of the protochlorophyllide present in unilluminated leaves; the net synthesis of chlorophyll during the first 3 The inhibitory effect of chloramphenicol on chlorophyll production seen here is similar to that on pigmentation of etiolated beans (18).…”

Section: Methodssupporting

confidence: 68%

Early Effects of Illumination on the Activity of Some Photosynthetic Enzymes

1967

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Sumimiitary. Leaves of dark-grown corn (Zea in'ays) were illulminated for periods ranging from 3 minutes to 12 hours. The changes in the activities of ribose-5-phosphate isomerase, ribulose-5-phosphate kinase, and ribulose-l,5-diphosphate carboxylase were followed.The activity of ribose-5-phosphate isomerase did ilot change significantly until between 12 and 24 hours of illumination. An increase in ribulose-5-phosphate kinase activity occturred after a lag of about 6 hours. The increase in carboxylase activity began after 3 minutes of illumination and increased uintil after 3 to 6 houtrs in the light, after which it began to decline. The increases in these enzymes appear to be the result of protein synthesis.The chloroplast, which has long been known to have at least partial genetic atutonomy, contains the components necessary for genetically directed protein synthesis. These include D'NA, RNA, ribosomes, and a soluble amino acid activating system ( 13,25,26,27,30). When etiolated leaves are exposed to light the proplastids uindergo great struictlural and biochemical changes in which nucleic acid and protein metabolism may be involved. Therefore it is of interest to follow the production of enzymes foulnd in the chloroplast and cytoplasm upon illuimination of leaves of dark-grown plants. The activities of 3 enzvmes which participate in the photosynthetic fixationi of carbon were sttudied in the present investigation: ribose-5-phosphate (R-5-P) isomerase; ribiilose-5-phosphate kinase; and ribulose-l,5-diphosphate (RuDP) carboxylase. The first of these is fouind both in the 1

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Section: Methodssupporting

confidence: 68%

Early Effects of Illumination on the Activity of Some Photosynthetic Enzymes

1967

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“…Etiolated p!ants were grown in the dark for 6 days at 250 (18 Thirtv g of leaves were soaked for 15 minutes at 1 Research was supported in part by funds provided by the United States Atomic Energy Commission Contract No. AT 2372.…”

Section: Methodsmentioning

confidence: 99%

In Vitro Protein Synthesis by Plastids of Phaseolus vulgaris. I. Localization of Activity in the Chloroplasts of a Chloroplast Containing Fraction from Developing Leaves

Parenti¹,

Margulies²

1967

Plant Physiol.

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“…bacillaris, strains Z and B, were cultured witll L-glutamate and DL-malate as carbon sources (8 (9). For development in light, the 6 day etiolated seedlings were exposed to a mixture of white and red fluorescent lamps.…”

Section: Methodsmentioning

confidence: 99%