Effect of chloride ions in Tris buffer solution on bioactive glass apatite mineralization

Kirste, Gloria; Brandt-Slowik, Juliane; Bocker, Christian; Steinert, Michael; Geiß, Reinhard; Brauer, Delia S.

doi:10.1111/ijag.12288

Cited by 20 publications

(15 citation statements)

References 51 publications

(63 reference statements)

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“…Kirste et al also compared two different Tris formulations, comparing ion release and apatite formation of 45S5 immersed in 0.062 mol L −1 Tris‐HCl and Tris‐HAc, where the pH was adjusted using acetic acid rather than hydrochloric acid. Both solutions had an initial pH of 7.4.…”

Section: Impact Of Immersion Media Compositionmentioning

confidence: 99%

A review of acellular immersion tests on bioactive glasses––influence of medium on ion release and apatite formation

Nommeots‐Nomm

Hupa

Rohanová

et al. 2020

Int J of Appl Glass Sci

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When evaluating new bioactive glass compositions for their suitability as a biomaterial, one of the first steps is the study of their behavior in contact with aqueous solutions. Ion release, pH changes, and apatite precipitation are investigated during immersion experiments, and a wide variety of solutions is used, including simulated body fluid, Tris buffer solution, various cell culture medium formulations or deionized water. This paper reviews the different parameters used for immersion experiments on bioactive glasses. Results show that, depending on solution composition, pH, and buffering capacity, the experimental outcome is likely to vary. In addition, bioactive glass particle size and solution volume/glass surface area ratio affect the resulting ion concentration in solution, and, thus, the rate at which apatite is formed. It is, therefore, important to consider these effects when planning experiments, interpreting results or comparing the results of experiments performed in different laboratories.

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Section: Impact Of Immersion Media Compositionmentioning

confidence: 99%

A review of acellular immersion tests on bioactive glasses––influence of medium on ion release and apatite formation

Nommeots‐Nomm

Hupa

Rohanová

et al. 2020

Int J of Appl Glass Sci

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“…5 show the FTIR spectra of 0B and 5B after SBF immersion with three different solution treatments (still, adjusted and refreshed), along with a FTIR spectrum of a commercial HAp powder for reference. The appearance of a split phosphate P–O bending band (∼560 and 600 cm −1 ), P–O stretching band (∼1015 cm −1 ) and a carbonate band (∼870 cm −1 ) indicates the formation of HAp [ 46 ]. For both 0B and 5B samples, refreshing SBF every 24 h promotes the HAp formation on glass surface, while still and adjusted treatments exhibit slower and similar HAp formation.…”

Section: Resultsmentioning

confidence: 99%

“…HCO 3 − concentration in SBF can affect the thickness of calcium phosphate formed [ 34 ], as well as the heterogeneity and the crystal size of the calcium phosphate precipitates [ 57 ]. Additionally, it was found that chloride ions in TRIS-HCl butter solution were incorporated in the apatite formation during immersion tests, affecting the final composition of precipitates [ 46 ]. Future detailed compositional studies of formed HAp are desired for better understanding the apatite formation mechanism, which can benefit the improvement of in vitro bioactivity evaluation of glass materials, as well as designing new functional biomaterials (e.g., apatite formed matches bone or dentin tissue [ 58 , 59 ]).…”

Section: Discussionmentioning

confidence: 99%

Effect of solution condition on hydroxyapatite formation in evaluating bioactivity of B2O3 containing 45S5 bioactive glasses

Kolzow

Chen

et al. 2019

Bioactive Materials

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The effects of testing solutions and conditions on hydroxyapatite (HAp) formation as a means of in vitro bioactivity evaluation of B 2 O 3 containing 45S5 bioactive glasses were systematically investigated. Four glass samples prepared by the traditional melt and quench process, where SiO 2 in 45S5 was gradually replaced by B 2 O 3 (up to 30%), were studied. Two solutions: the simulated body fluid (SBF) and K 2 HPO 4 solutions were used as the medium for evaluating in vitro bioactivity through the formation of HAp on glass surface as a function of time. It was found that addition of boron oxide delayed the HAp formation in both SBF and K 2 HPO 4 solutions, while the reaction between glass and the K 2 HPO 4 solution is much faster as compared to SBF. In addition to the composition and medium effects, we also studied whether the solution treatments (e.g., adjusting to maintain a pH of 7.4, refreshing solution at certain time interval, and no disturbance during immersion) affect HAp formation. Fourier transform infrared spectrometer (FTIR) equipped with an attenuated total reflection (ATR) sampling technique and scanning electron microscopy (SEM) were conducted to identify HAp formation on glass powder surfaces and to observe HAp morphologies, respectively. The results show that refreshing solution every 24 h produced the fastest HAp formation for low boron-containing samples when SBF was used as testing solution, while no significant differences were observed when K 2 HPO 4 solution was used. This study thus suggests the testing solutions and conditions play an important role on the in vitro bioactivity testing results and should be carefully considered when study materials with varying bioactivities.

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“…[45][46][47] Indeed, SBF has much more ions dissolved, thus being more saturated and prone to precipitation reactions. Instead, bioactivity behavior was favored in rare earth-containing glasses.…”

Section: Discussionmentioning

confidence: 99%

“…The causes of such different behavior between SBF and TRIS-HCl solution have been attributed to solution ionic strength and supersaturation. [45][46][47] Indeed, SBF has much more ions dissolved, thus being more saturated and prone to precipitation reactions. 28 Besides, silicon concentration in SBF is at least ten times lower than in TRIS-HCl solution, indicating that the ionic strength of SBF solution has slowed glass dissolution, and the effect of rare earth on glass dissolution is not pronounced.…”

Section: Discussionmentioning

confidence: 99%

Dissolution, bioactivity behavior, and cytotoxicity of rare earth‐containing bioactive glasses (RE = Gd, Yb)

Zambanini

Borges

Faria

et al. 2019

Int J Applied Ceramic Tech

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Rare earth‐containing bioactive glasses (RE‐BGs) have been poorly explored in the biomaterials field, although RE has optical, nuclear, and magnetic properties that could be used in different biomedical applications. In order to verify whether these glasses can be promising as biomaterials, we studied the dissolution, bioactivity, and cytotoxicity of RE‐BGs based on the SiO2–Na2O–CaO–P2O5–RE2O3 (RE = Gd, Yb) system. The glasses were obtained by melting‐quenching and their particle size was determined by laser diffraction. Their dissolution behavior was studied in Tris‐HCl, while bioactivity was performed in simulated body fluid solution under physiological conditions during several periods. The cytotoxicity test was performed using glass‐derived conditioned medium and mesenchymal stem cell derived from deciduous teeth. The dissolution results showed that the glasses dissolved under two different kinetics, which are lower for rare earth‐containing glasses, due to the more covalent character of Si–O–RE bonds. The bioactivity results evidenced that all glasses showed bioactivity after 24 hours. However, gadolinium and ytterbium promoted a more calcium phosphate deposition, which contrasts with the slower dissolution kinetics of rare earth‐containing glasses. All the glasses were considered biocompatible, showing cell viability higher than 80%. The overall results showed that RE‐BGs are promising materials for applications that require bioactivity and/or biocompatibility.

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Effect of chloride ions in Tris buffer solution on bioactive glass apatite mineralization

Cited by 20 publications

References 51 publications

A review of acellular immersion tests on bioactive glasses––influence of medium on ion release and apatite formation

A review of acellular immersion tests on bioactive glasses––influence of medium on ion release and apatite formation

Effect of solution condition on hydroxyapatite formation in evaluating bioactivity of B2O3 containing 45S5 bioactive glasses

Dissolution, bioactivity behavior, and cytotoxicity of rare earth‐containing bioactive glasses (RE = Gd, Yb)

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