Effect of Chlorinated Ethenes on <i>S</i><sub>min</sub> for a Methanotrophic Mixed Culture

Anderson, James E.; McCarty, Perry L.

doi:10.1021/es9606687

Cited by 7 publications

(2 citation statements)

References 42 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In fact, the concept of operational transformation yield is equivalent to the previously introduced calculation of the minimum substrate concentration required per mass of chlorinated solvent degraded for sustained microbial growth during chlorinated solvent degradation ( − ). As shown in Figures and , the operational transformation yields for >99% cDCE removal are 5−7-fold higher than those for TCE, suggesting that cDCE exerts less product toxicity than TCE.…”

Section: Discussionmentioning

confidence: 99%

Treatment of Chlorinated Solvents by Nitrogen-Fixing and Nitrate-Supplied Methane Oxidizers in Columns Packed with Unsaturated Porous Media

Chu

Alvarez‐Cohen

2000

Environ. Sci. Technol.

View full text Add to dashboard Cite

This study compares the feasibility of employing nitrogen-fixing and nitrate-supplied methane-oxidizing cultures grown in unsaturated porous media to degrade cis-1,2-dichloroethylene (cDCE) and trichloroethylene (TCE) in gas streams. Both nitrate-supplied and nitrogen-fixing columns (supplied with 10% CH4 and 10% O2) degraded TCE completely at a gaseous concentration of 0.7 mg/L for 8−10 days. However, when columns were supplied with 4% CH4 and 10% O2, nitrate-supplied columns were not able to recover after degrading TCE at a gaseous concentration of 0.13 mg/L for 7 days. In contrast, nitrogen-fixing columns recovered after degrading 0.13−0.4 mg/L TCE for 3−10 days and were capable of repeatedly degrading TCE at gaseous concentrations of 0.03−0.14 mg/L during long-term intermittent operation (lasting from 38 to 84 days) that was punctuated by appropriate microbial recovery periods (7−9 days). Both nitrate-supplied and nitrogen-fixing columns were capable of degrading cDCE at concentrations of 0.7−1.0 mg/L for 5−10 days, but only the nitrogen-fixing columns recovered from cDCE exposure. The operating period for columns treating a mixture of TCE and cDCE was significantly shorter than that for treatment of TCE or cDCE alone. Several operating curves (percent chlorinated solvent removal versus mass of chlorinated solvent loaded per mass of methane consumed) were developed to facilitate comparisons between operating conditions and to aid in predicting chlorinated solvent removals in such systems. Nitrogen-fixing columns consistently outperformed nitrate-supplied columns, and columns inoculated with a mixed culture outperformed those inoculated with Methylosinus trichosporium OB3b for TCE removal but not for cDCE removal.

show abstract

Section: Discussionmentioning

confidence: 99%

Treatment of Chlorinated Solvents by Nitrogen-Fixing and Nitrate-Supplied Methane Oxidizers in Columns Packed with Unsaturated Porous Media

Chu

Alvarez‐Cohen

2000

Environ. Sci. Technol.

View full text Add to dashboard Cite

show abstract

“…However, the model did not predict TCE degradation by resting cells. The presence of tri-and dichloroethylenes increased the minimum methane concentration for net growth of a methanotrophic mixed culture by a factor of five (Anderson and McCarty, 1997). Smith et al (1997) determined rates of methane and TCE use by a methanolrophic mixed culture using data from batch and pseudo-steady-state data and summarized previously reported values of rate coefficients for methanotrophic cultures.…”

mentioning

confidence: 99%