Effect of chlorination and ultraviolet disinfection on tetA-mediated tetracycline resistance of Escherichia coli

Huang, Jingyun; Hu, Hong-Ying; Wu, Yin-Hu; Wei, Bin; Lu, Yun

doi:10.1016/j.chemosphere.2012.10.008

Cited by 108 publications

(61 citation statements)

References 26 publications

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“…However, studies have reported the survival of E. coli survival in chlorinated effluents [30,31]. In the study by [32] the tolerance of E. coli CGMCC to 10 mg/L chlorine dose was observed with only 4.0 log reduction achieved in a bacterial population of approximately 10 7 colony forming units (CFU/mL).…”

Section: Introductionmentioning

confidence: 98%

Chlorine Tolerance and Inactivation of Escherichia coli recovered from Wastewater Treatment Plants in the Eastern Cape, South Africa

2017

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Featured Application: This study examined the tolerance of Escherichia coli isolates, a fecal indicator of water contamination, to chlorine as a common water disinfectant and the effectiveness of chlorine at different concentrations in eliminating E. coli in wastewater effluent. Data obtained can be used as baseline monitoring data for future epidemiological surveillances that could further enhance the control of E. coli and some bacteria species of public health concern in wastewater treatment plants and ensure protection of public and environmental health.Abstract: This study investigated the survival of Escherichia coli (E. coli) recovered from secondary effluents of two wastewater treatment plants in the Eastern Cape Province, South Africa, in the presence of different chlorine concentrations. The bacterial survival, chlorine lethal dose and inactivation kinetics at lethal doses were examined. The bacterial isolates were identified by 16S rRNA gene sequencing. Comparison of the nucleotide sequences of 16S rRNA gene of bacteria with known taxa in the GenBank revealed the bacterial isolates to belong to Escherichia coli. At the recommended free chlorine of 0.5 mg/L, reduction of E. coli isolates (n = 20) initial bacterial concentration of 8.35-8.75 log was within a range of 3.88-6.0 log at chlorine residuals of 0.14-0.44 mg/L after 30 min. At higher doses, a marked reduction (p < 0.05) in the viability of E. coli isolates was achieved with a greater than 7.3 log inactivation of the bacterial population. Inactivation kinetics revealed a high rate of bacterial kill over time (R 2 > 0.9) at chlorine dose of 1.5 mg/L. This study indicates poor removal of bacteria at free chlorine at 0.5 mg/L and a greater efficacy of 1.5 mg/L in checking E. coli tolerance.

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Section: Introductionmentioning

confidence: 98%

Chlorine Tolerance and Inactivation of Escherichia coli recovered from Wastewater Treatment Plants in the Eastern Cape, South Africa

2017

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“…More recently, Huang et al [56] showed that the inactivation rates of ARB (namely, chloramphenicol-, rifampicin-, tetracycline-resistant bacteria) were not lower than that of total heterotrophic bacteria, even if the proportion of several ARB increased after chlorination. The same group also showed that the chlorination process increased the average tetracycline resistance of tetracycline-resistant E. coli [62]. Particularly, the inactivation rates of tetracycline-resistant E. coli were lower than those of antibiotic-sensitive E. coli when the initial concentration of chlorine was higher than 1.5-mg Cl 2 L À1 .…”

Section: Chlorinationmentioning

confidence: 90%

“…Heterotrophic ARB (cephalexin, ciprofloxacin, erythromycin, gentamicin, vancomycin, sulfadiazine, rifampicin, tetracycline and chloramphenicol) UV radiation 5 mJ cm The average tetracycline resistance increased [62] UV radiation 10 mJ cm MDR multidrug resistant chemicals [55]. In this section, the effect of these disinfection processes on the inactivation of resistant microorganisms is briefly reviewed, and the relevant data (disinfectant dose and inactivation rate) are summarised in Table 3.…”

Section: Wastewater Disinfection By Conventional Processes: Effect Onmentioning

confidence: 99%

Conventional and New Processes for Urban Wastewater Disinfection: Effect on Emerging and Resistant Microorganisms

Ferro

Polo-López

Fernández‐Ibáñez

2015

The Handbook of Environmental Chemistry

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The continuous release of chemical and microbiological pollutants into the environment and the increasing demand for safe water call for effective water and wastewater treatment processes. In particular, the detection of resistant microorganisms (e.g. antibiotic-resistant bacteria) in the effluents of urban wastewater treatment plants disposed into surface water or reused (e.g. in crop irrigation) shows that conventional treatments and disinfection processes do not effectively control the spread of pathogens into the environment. There is a need for new and more effective disinfection processes and technologies. The aim of this chapter is to briefly describe some of the emerging and antimicrobial-resistant microorganisms detected in wastewater, as well as the conventional and new advanced available technologies for wastewater disinfection, and to evaluate and discuss their effect on these microorganisms. Moreover, regulations and policies on wastewater reuse are also critically discussed and compared.

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“…Additionally, the proportion of multidrug-resistant bacteria isolated from the chlorinated effluent increases compared to the influent, and antibiotic-resistance genes remain detectable in the chlorinated effluent [25]. Huang et al [26] also indicated that tetracycline-resistant E. coli showed a higher resistance to chlorine than tetracycline-sensitive E. coli. These results suggested that the tolerance to chlorine may vary depending on the type of ARB.…”

Section: Inactivation Ratio Of Vre and Detection Of Vana In Secondarymentioning

confidence: 99%

“…Our results showed that the effect of chlorine disinfection could not destroy antibiotic-resistance genes, though it could inactivate and decrease bacterial counts of ARB. On the other hand, it has been reported that UV can decrease ARB more effectively than chlorination [21,26]. Sequential UV/chlorination, in particular, enhanced the inactivation of antibiotic resistance genes [27].…”

Section: Time (Min)mentioning

confidence: 99%

Inactivation Effect of Antibiotic-Resistant Gene Using Chlorine Disinfection

Furukawa

Jikumaru

Ueno

et al. 2017

Water

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Abstract:The aim of this study was to elucidate the inactivation effects on the antibiotic-resistance gene (vanA) of vancomycin-resistant enterococci (VRE) using chlorination, a disinfection method widely used in various water treatment facilities. Suspensions of VRE were prepared by adding VRE to phosphate-buffered saline, or the sterilized secondary effluent of a wastewater treatment plant. The inactivation experiments were carried out at several chlorine concentrations and stirring time. Enterococci concentration and presence of vanA were determined. The enterococci concentration decreased as chlorine concentrations and stirring times increased, with more than 7.0 log reduction occurring under the following conditions: 40 min stirring at 0.5 mg Cl 2 /L, 20 min stirring at 1.0 mg Cl 2 /L, and 3 min stirring at 3.0 mg Cl 2 /L. In the inactivation experiment using VRE suspended in secondary effluent, the culturable enterococci required much higher chlorine concentration and longer treatment time for complete disinfection than the cases of suspension of VRE. However, vanA was detected in all chlorinated suspensions of VRE, even in samples where no enterococcal colonies were present on the medium agar plate. The chlorine disinfection was not able to destroy antibiotic-resistance genes, though it can inactivate and decrease bacterial counts of antibiotic-resistant bacteria (ARB). Therefore, it was suggested that remaining ARB and/or antibiotic-resistance gene in inactivated bacterial cells after chlorine disinfection tank could be discharged into water environments.

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Effect of chlorination and ultraviolet disinfection on tetA-mediated tetracycline resistance of Escherichia coli

Cited by 108 publications

References 26 publications

Chlorine Tolerance and Inactivation of Escherichia coli recovered from Wastewater Treatment Plants in the Eastern Cape, South Africa

Chlorine Tolerance and Inactivation of Escherichia coli recovered from Wastewater Treatment Plants in the Eastern Cape, South Africa

Conventional and New Processes for Urban Wastewater Disinfection: Effect on Emerging and Resistant Microorganisms

Inactivation Effect of Antibiotic-Resistant Gene Using Chlorine Disinfection

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