Effect of Chlorpyrifos Ethyl on Acetylcholinesterase Activity in Climbing Perch (Anabas testudineus, Bloch, 1972)

Abstract

The high use of pesticides in intensive rice farming in the Mekong Delta constitutes a potential hazard to the environment and to people's health. Chlorpyrifos ethyl (CPF) is a commonly used organophosphate (OP) insecticide, but information about its potential negative impacts on the aquatic environment in the Mekong Delta is scarce. Both acute and subacute toxicity tests were performed in a static nonrenewable system to investigate the effects of CPF on brain acetylcholinesterase (AChE) activity in native climbing perch fingerlings (Anabas testudineus, Bloch, 1972). Environmental parameters, such as dissolved oxygen, water temperature, and pH, were similar to field conditions in the Mekong Delta. In a 96-h lethal concentration (LC50) test, fingerlings of climbing perch were randomly exposed to five levels of CPF ranging from 0.8 to 4.5 ppm. Five sublethal levels of CPF (1, 5, 10, 15, and 20 % of the 96-h LC50 value) were tested to assess the sensitivity and recovery of the brain AChE activity in climbing perch fingerlings exposed to CPF. The results showed that CPF were moderately toxic to climbing perch with a 96-h median LC50 of 1.73 ppm. CPF also caused long-term AChE inhibition with 70 % inhibition remaining after 96 h for the four highest test concentrations. The recovery of brain AChE activity in fish placed in CPF-free water was very slow, and after 7 days the brain AChE activity was still significant lower in fish from the four highest concentrations compared with the control. The results from this study indicate that OP insecticides, such as CPF, can have long-lasting sublethal effects on aquatic species in the Mekong Delta.

Temefos’a Maruz Kalan Gökkuşağı Alabalıklarında, Antioksidan Cevaplar

“…AChE was present in the brain tissue of trout at the lowest TE concentration (3 mg L -1 ) and the enzyme activity in brain is sensitive to the inhibitory effects of TE. There are a large number of studies focusing on the connection between pesticides, organophosphates, and AChE activity in different species (Tam et al, 2015;Petrov et al, 2018). However, there is no study focusing on the relationship between TE and AChE activity in fish.…”

TOXICIDAD AGUDA DEL MALATION 500® Y TYSON 4E® EN Capitella sp.

“…López-Aca et al (2018) realizaron bioensayos de toxicidad aguda a 48 h con la especie Odontesthes bonariensis y registraron una CL 50 de 0.007 mg/L. En otro estudio, Tam et al (2015) encontraron en la especie Anabas testudineus un valor de CL 50 a 48 h de 2.00 mg/L.…”

The combined effect of Bassa 50EC and Vitashield 40EC on the brain acetylcholinesterase activity in climbing perch (Anabas testudineus)

“…All concentrations, including the control, were done with three replicates. The used concentrations of the pesticides corresponded to 10% of the 96-h LC 50 values of CPF (Tam et al 2015b ) and F (Lan 2004 ) for climbing perch fingerlings.…”