Effect of cholesterol concentration on size of liposome

Pathak, Sarvesh Kumar; Mishra, Roli; Kumar, Sandeep; Prakash, Gyan; Parthasarthy, R.

doi:10.9790/3008-0115053

Cited by 8 publications

(5 citation statements)

References 11 publications

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“…Regarding the cholesterol content in the formulation, a highly significant ( p ≤ 0.0001) decrease in the liposomes’ size occurred when the cholesterol content increased. This finding agrees with the results obtained by Pathak et al, who investigated the effect of the cholesterol concentration on the liposomes’ size [ 67 ].…”

Section: Resultssupporting

confidence: 93%

Development and Optimisation of Inhalable EGCG Nano-Liposomes as a Potential Treatment for Pulmonary Arterial Hypertension by Implementation of the Design of Experiments Approach

et al. 2023

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Epigallocatechin gallate (EGCG), the main ingredient in green tea, holds promise as a potential treatment for pulmonary arterial hypertension (PAH). However, EGCG has many drawbacks, including stability issues, low bioavailability, and a short half-life. Therefore, the purpose of this research was to develop and optimize an inhalable EGCG nano-liposome formulation aiming to overcome EGCG’s drawbacks by applying a design of experiments strategy. The aerodynamic behaviour of the optimum formulation was determined using the next-generation impactor (NGI), and its effects on the TGF-β pathway were determined using a cell-based reporter assay. The newly formulated inhalable EGCG liposome had an average liposome size of 105 nm, a polydispersity index (PDI) of 0.18, a zeta potential of −25.5 mV, an encapsulation efficiency of 90.5%, and a PDI after one month of 0.19. These results are in complete agreement with the predicted values of the model. Its aerodynamic properties were as follows: the mass median aerodynamic diameter (MMAD) was 4.41 µm, the fine particle fraction (FPF) was 53.46%, and the percentage of particles equal to or less than 3 µm was 34.3%. This demonstrates that the novel EGCG liposome has all the properties required to be inhalable, and it is expected to be deposited deeply in the lung. The TGFβ pathway is activated in PAH lungs, and the optimum EGCG nano-liposome inhibits TGFβ signalling in cell-based studies and thus holds promise as a potential treatment for PAH.

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Section: Resultssupporting

confidence: 93%

Development and Optimisation of Inhalable EGCG Nano-Liposomes as a Potential Treatment for Pulmonary Arterial Hypertension by Implementation of the Design of Experiments Approach

et al. 2023

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“…Variations of the PC:CH molar ratio did not affect significantly the vesicles properties (Mohammed et al, 2004). However, a slight reduction in vesicle size can be observed with increasing the CH amount, probably related to its ability to enhance the bilayer rigidity and, consequently, reduce the vesicles dimensions (López-Pinto et al, 2005;Pathak et al, 2012).…”

Section: Chitosomespreparation and Characterizationmentioning

confidence: 83%

Calcium alginate microspheres containing metformin hydrochloride niosomes and chitosomes aimed for oral therapy of type 2 diabetes mellitus

Maestrelli

Mura

González-Rodrı́guez

et al. 2017

International Journal of Pharmaceutics

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“…Furthermore, its proportion with respect to the phospholipid in membranes affects the drug encapsulation efficiency [27][28][29]. However, there are few reports regarding the ratio between cholesterol and phospholipid used in L formulation [26,28,[30][31][32], and there are even fewer regarding the ratio cholesterol/phospholipid to provide a controlled drug release [26,28,30].…”

Section: Introductionmentioning

confidence: 99%

Cholesterol Levels Affect the Performance of AuNPs-Decorated Thermo-Sensitive Liposomes as Nanocarriers for Controlled Doxorubicin Delivery

et al. 2021

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Stimulus-responsive liposomes (L) for triggering drug release to the target site are particularly useful in cancer therapy. This research was focused on the evaluation of the effects of cholesterol levels in the performance of gold nanoparticles (AuNPs)-functionalized L for controlled doxorubicin (D) delivery. Their interfacial and morphological properties, drug release behavior against temperature changes and cytotoxic activity against breast and ovarian cancer cells were studied. Langmuir isotherms were performed to identify the most stable combination of lipid components. Two mole fractions of cholesterol (3.35 mol% and 40 mol%, L1 and L2 series, respectively) were evaluated. Thin-film hydration and transmembrane pH-gradient methods were used for preparing the L and for D loading, respectively. The cationic surface of L allowed the anchoring of negatively charged AuNPs by electrostatic interactions, even inducing a shift in the zeta potential of the L2 series. L exhibited nanometric sizes and spherical shape. The higher the proportion of cholesterol, the higher the drug loading. D was released in a controlled manner by diffusion-controlled mechanisms, and the proportions of cholesterol and temperature of release media influenced its release profiles. D-encapsulated L preserved its antiproliferative activity against cancer cells. The developed liposomal formulations exhibit promising properties for cancer treatment and potential for hyperthermia therapy.

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Effect of cholesterol concentration on size of liposome

Cited by 8 publications

References 11 publications

Development and Optimisation of Inhalable EGCG Nano-Liposomes as a Potential Treatment for Pulmonary Arterial Hypertension by Implementation of the Design of Experiments Approach

Development and Optimisation of Inhalable EGCG Nano-Liposomes as a Potential Treatment for Pulmonary Arterial Hypertension by Implementation of the Design of Experiments Approach

Calcium alginate microspheres containing metformin hydrochloride niosomes and chitosomes aimed for oral therapy of type 2 diabetes mellitus

Cholesterol Levels Affect the Performance of AuNPs-Decorated Thermo-Sensitive Liposomes as Nanocarriers for Controlled Doxorubicin Delivery

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