Effect of cholesterol on lipogenesis and VLDL–TG assembly and secretion in goose primary hepatocytes

Han, Chunchun; Wang, J. W.; Pan, Zhenxiao; Tang, Huaizhi; Xiang, Shuxia; Li, Liang; Xu, Funeng; Wei, Shuhong

doi:10.1007/s11010-012-1516-3

Cited by 11 publications

(9 citation statements)

References 32 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Most apolipoproteins, endogeneous lipids, and lipoproteins are synthesized in the liver, which depend on the cellular integrity and functions (Han et al, 2013). In the present study, treatment of male rats with FLU (10 mg or 500 mg/kg) exhibited significant increases in both serum and liver TL, TC and TG in addition to serum LDL-C levels, in a dose dependent manner, when compared to the control group.…”

Section: Discussionsupporting

confidence: 50%

Exploring the protective effect of silymarin against flutamide-induced hepatotoxicity in male rats

2016

Res. Opin. Anim. Vet. Sci.

View full text Add to dashboard Cite

The current study was designed to explore the protective impact of silymarin (SIL) against the hepatotoxicity induced by daily treatment with flutamide (FLU), for one month, in intact male rats. Two different doses of FLU, one was low and corresponds to the human therapeutic dose (10 mg/kg) and the other was high and corresponds to ½ lowest lethal dose (500 mg/kg) were applied. Obtained results showed that oral administration of FLU in male rats significantly increased hepatic malonic dialdehyde (MDA), and markedly decreased hepatic reduced glutathione (GSH), superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx) and glutathione-stranseferase (GST). In parallel, activities of alanine aminotranseferase (ALT), aspartate aminotranseferase (AST) and alkaline phosphatase (ALP), and level of total bilirubin in serum were significantly elevated; while serum total proteins, albumin and globulin, and hepatic total proteins were decreased in FLU-treated animals. Also, significant increases in serum and liver contents of total lipids (TL), total cholesterol (TC) and triglycerides (TG), and serum low density lipoproteins-cholesterol (LDL-c), which accompanied with decrease in serum level of high density lipoproteinscholesterol (HDL-c) following administration of FLU. The adverse effects of FLU on measured parameters appeared to be dose-dependent. Treatment with SIL (50 mg/kg) in rats given FLU (10 mg or 500 mg/kg) greatly improved the levels of investigated biomarkers of liver status. However, the beneficial effect of SIL was more remarkable in rats receiving FLU at a dose of 10 mg/kg. Obtained biochemical changes induced by the two used doses of FLU and the ameliorative impact of SIL were confirmed by histopathological examinations of liver sections of treated rats. In conclusion, SIL can be used effectively in the protection against hepatotoxicity induced by FLU.

show abstract

Section: Discussionsupporting

confidence: 50%

Exploring the protective effect of silymarin against flutamide-induced hepatotoxicity in male rats

2016

Res. Opin. Anim. Vet. Sci.

View full text Add to dashboard Cite

show abstract

“…Excessive production of TG-rich VLDL (VLDL-TG) contributes to hypertriglyceridemia [30]. Many genes are involved in the assembly and secretion of VLDL-TG, such as the microsomal triglyceride transfer protein (MTP) and apolipoprotein A-V (apoA-V) [31,32]. Previous studies showed that testosterone regulates hepatic MTP expression in rats and mice [25,33].…”

Section: Discussionmentioning

confidence: 99%

Effect of testosterone deficiency on cholesterol metabolism in pigs fed a high-fat and high-cholesterol diet

et al. 2015

View full text Add to dashboard Cite

BackgroundTestosterone deficiency is associated with increased serum cholesterol levels. However, how testosterone deficiency precisely affects cholesterol metabolism remains unclear. Therefore, in the current study, we examined the effect of testosterone deficiency on cholesterol metabolism and liver gene expression in pigs fed a high-fat and high-cholesterol (HFC) diet.MethodsSexually mature male miniature pigs (6–7 months old) were randomly divided into 3 groups as follows: intact male pigs fed an HFC diet (IM + HFC), castrated male pigs fed an HFC diet (CM + HFC), and castrated pigs with testosterone replacement fed an HFC diet (CM + HFC + T). Serum testosterone levels and lipid profiles were measured, and gene expression levels associated with hepatic cholesterol metabolism were determined. Furthermore, total hepatic cholesterol contents and the activities of enzymes mediating hepatic cholesterol metabolism were measured.ResultsSerum testosterone levels were significantly decreased in CM + HFC pigs, and testosterone replacement attenuated castration-induced testosterone deficiency. Castration significantly increased the serum levels of total cholesterol, low-density lipoprotein cholesterol and triglycerides, as well as hepatic lipid contents in pigs fed an HFC diet. Compared with IM + HFC and CM + HFC + T pigs, low-density lipoprotein receptor (LDLR) mRNA expression and protein levels were significantly decreased in the livers of CM + HFC pigs. In contrast, we found that compared with IM + HFC pigs, hepatic proprotein convertase subtilisin/kexin type 9 (PCSK9) mRNA and serum PCSK9 protein levels were significantly increased in CM + HFC pigs. Moreover, testosterone treatment reversed the increase in PCSK9 expression in CM + HFC pigs. However, neither castration nor testosterone replacement affected the expression of the other hepatic genes that were tested.ConclusionsThis study demonstrated that castration-induced testosterone deficiency caused severe hypercholesterolemia in pigs fed an HFC diet; furthermore, these effects could be reversed by testosterone replacement therapy. Altered hepatic PCSK9 and LDLR expression, resulting in reduced LDL-cholesterol clearance, may contribute to the increased serum cholesterol levels induced by testosterone deficiency and an HFC diet. These results deepen our understanding of the underlying molecular mechanisms that mediate the effects of testosterone deficiency on cholesterol metabolism.

show abstract

“…There have studies demonstrated that the mRNA expressions of fatty acid synthase (FAS), acetyl‐CoA carboxylase‐ α (ACC α ), sterol regulatory element binding protein‐1c (SREBP‐1c) and extracellular VLDL concentration in goose primary hepatocytes were increased after incubated with cholesterol (Han et al., ). Mannisto et al.…”

Section: Discussionmentioning

confidence: 99%

Effects of β‐hydroxybutyricacid on the synthesis and assembly of very low‐density lipoprotein in bovine hepatocytes in vitro

Deng

Huang

et al. 2015

Animal Physiology Nutrition

View full text Add to dashboard Cite

β-Hydroxybutyricacid (BHBA) is an important metabolite that involved in the development of ketosis and fatty liver in dairy cows. Dairy cows with fatty liver displayed high blood concentration of BHBA and very low-density lipoprotein (VLDL) assembly. The effects of BHBA on VLDL synthesis and assembly in hepatocytes of cows were unclear. In this study, bovine hepatocytes were cultured and treated with different concentrations of BHBA. We found that BHBA treatment upregulated the mRNA and protein levels of apolipoprotein B100 (ApoB 100), apolipoprotein E (ApoE) and microsomal triglyceride transfer protein (MTTP) and showed in a firstly increased and then decreased trend. Meanwhile, the mRNA and protein levels of LDLR showed in a reverse trend. Consequently, VLDL content was significantly increased in medium-dose BHBA treatment group, while decreased in high-dose group. These results indicate that the effects of BHBA on the VLDL synthesis showed in a dose-dependent manner that low levels of BHBA increase VLDL synthesis and high levels of BHBA decrease VLDL synthesis.

show abstract

Effect of cholesterol on lipogenesis and VLDL–TG assembly and secretion in goose primary hepatocytes

Cited by 11 publications

References 32 publications

Exploring the protective effect of silymarin against flutamide-induced hepatotoxicity in male rats

Exploring the protective effect of silymarin against flutamide-induced hepatotoxicity in male rats

Effect of testosterone deficiency on cholesterol metabolism in pigs fed a high-fat and high-cholesterol diet

Effects of β‐hydroxybutyricacid on the synthesis and assembly of very low‐density lipoprotein in bovine hepatocytes in vitro

Contact Info

Product

Resources

About