Effect of Cholesterol Versus Ergosterol on DPPC Bilayer Properties: Insights from Atomistic Simulations

Alavizargar, Azadeh; Keller, Fabian; Wedlich‐Söldner, Roland; Heuer, Andreas

doi:10.1021/acs.jpcb.1c03528

Cited by 11 publications

(11 citation statements)

References 70 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The higher bilayer thickness of DPPC-rich L o regions can be attributed to the presence of cholesterol, which disrupts the lipid tilt. 43 Although the presence of two phases was evident in the SAXS region, they could not be clearly resolved from the WAXS diffraction pattern (Fig. 4d), thus preventing an accurate estimation of the respectively, in good agreement with previous reports (~67Å 2 and 52 Å 2 for L d and L o regions).…”

Section: Resultssupporting

confidence: 88%

Viscosity-sensitive membrane dyes as tools to estimate the crystal-line structure of lipid bilayers

Paez-Perez

Dent

Brooks

et al. 2023

Preprint

View full text Add to dashboard Cite

Lipid membranes are crucial for cellular metabolism, and their correct function has been linked to a tight regulation of their structural and mechanical properties, such as viscosity. Fluorescent probes sensitive to the membrane’s environ- are being extensively used to investigate the membrane’s properties, yet there is currently a lack of understanding on how the lipid organization impacts the readout from these dyes. Here, we investigate this relationship by simultaneously characterizing the membrane’s viscosity and structural properties using a combination of X-Ray diffraction, together with environmentally-sensitive optical membrane probes and fluorescence lifetime imaging microscopy. Our results reveal a phase-dependent connection between the different membrane’s structural and mechanical parameters and give insight into the relationship between two widely used membrane probes with the structural descriptors of the lipid bi-layer. Such relationship is believed to dictate the lateral organization of lipid bilayers, including the presence of distinct lipid domains which have been traditionally ascribed to a difference in the membrane thickness of the lipid phases; yet we later demonstrate how such connection is not universal and can disrupted by the presence of line-active molecules. Our results show the capability of membrane dyes to directly report on the membrane’s molecular structure – after appropriate calibration – and highlight the need of multiple orthogonal characterization strategies for a proper understanding of the membrane’s properties.

show abstract

Section: Resultssupporting

confidence: 88%

Viscosity-sensitive membrane dyes as tools to estimate the crystal-line structure of lipid bilayers

Paez-Perez

Dent

Brooks

et al. 2023

Preprint

View full text Add to dashboard Cite

show abstract

“…The higher bilayer thickness of DPPC-rich L o regions can be attributed to the presence of cholesterol, which disrupts the lipid tilt. 49 Although the presence of two phases was evident in the SAXS region, they could not be clearly resolved from the WAXS diffraction pattern (Fig. 4d), thus preventing an accurate estimation of the lipid-lipid dis- respectively, in good agreement with previous reports (~67Å 2 and 52 Å 2 for L d and L o regions).…”

Section: Combined Xrd/flim Characterization Of Ternary Lipid Mixtures...supporting

confidence: 86%

Viscosity-sensitive membrane dyes as tools to estimate the crystalline structure of lipid bilayers

Paez-Perez

Dent

Brooks

et al. 2023

Preprint

View full text Add to dashboard Cite

Lipid membranes are crucial for cellular integrity and regulation, and tight control of their structural and mechanical properties is vital to ensure that they function properly. Fluorescent probes sensitive to the mem-brane’s microenvironment are useful for investigating lipid membrane properties, however, there is currently a lack of quantitative correlation between the exact parameters of lipid organization and a readout from these dyes. Here, we investigate this relationship for ‘molecular rotors’, or microviscosity sensors, by simultaneously measuring their fluorescence lifetime to determine the membrane viscosity, while using the X-Ray diffraction the determine the membrane’s structural properties. Our results reveal a phase-dependent correlation be-tween the membrane’s structural parameters and mechanical properties measured by a BODIPY-based mo-lecular rotor, giving excellent predictive power for the structural descriptors of the lipid bilayer. We also demonstrate that differences in membrane thickness between different lipid phases is not a prerequisite for formation of lipid microdomains and that this requirement can be disrupted by the presence of line-active molecules. Our results underpin the use of membrane-sensitive dyes as reporters of the structure of lipid membranes.

show abstract

“…A tetracyclic fused ring along with a flexible hydrocarbon side chain helps to attract phospholipid acyl chains. As a result, an ordered structure is formed, which is strongly stabilized by the van der Waals cohesive interactions between the sterol rings of Chol molecules and the alkyl chains of DPPC [ 31 , 40 ]. Therefore, the presence of Chol molecules has a condensing effect on the DPPC and DPPC + MPs monolayers.…”

Section: Resultsmentioning

confidence: 99%

Effect of Silica Microparticles on Interactions in Mono- and Multicomponent Membranes

Tim

Rojewska

Prochaska

2022

IJMS

View full text Add to dashboard Cite

Advancing our understanding of the mechanism of the interaction between inhaled pollutant microparticles and cell membrane components is useful to study the impact of fine particulate matter on human health. In this paper, we focus on the effect of cholesterol (Chol) molecules on the surface properties of a model membrane in the presence of silica microparticles (MPs). Mixed monolayers containing phospholipid-dipalmitoylphosphatidylcholine (DPPC), Chol and silica particle dispersions (MPs; 0.033% w/w, 0.33% w/w and 0.83% w/w) were formed and studied using the Langmuir monolayer technique complemented by Brewster Angle Microscopy (BAM) images. It was shown that Chol caused a condensation of the DPPC monolayer, which influenced the penetration of MPs and their interactions with the model membrane. The relaxation experiments of the lipid–MP monolayer proved that the presence of Chol molecules in the monolayer led to the formation of lipid and MP complexes. Strong interactions between Chol and MPs contributed to the formation of more stable monolayers. The presented results can be useful to better comprehend the interaction between particulate materials and the lipid components of biomembranes.

show abstract

Effect of Cholesterol Versus Ergosterol on DPPC Bilayer Properties: Insights from Atomistic Simulations

Cited by 11 publications

References 70 publications

Viscosity-sensitive membrane dyes as tools to estimate the crystal-line structure of lipid bilayers

Viscosity-sensitive membrane dyes as tools to estimate the crystal-line structure of lipid bilayers

Viscosity-sensitive membrane dyes as tools to estimate the crystalline structure of lipid bilayers

Effect of Silica Microparticles on Interactions in Mono- and Multicomponent Membranes

Contact Info

Product

Resources

About