Effect of chondroitin sulfate and hyaluronic acid on gene expression in a three-dimensional culture of chondrocytes

Nishimoto, Shohei; Takagi, Mutsumi; Wakitani, Shigeyuki; Nihira, Takuya; Yoshida, Takemi

doi:10.1263/jbb.100.123

Cited by 42 publications

(34 citation statements)

References 6 publications

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“…On the other hand, the effect of CS seems to be due by the inhibition of both the nuclear translocation of NF-kβ and targeting TLR4 and CD44, thus preventing ECM-f from unleashing the inflammatory cascade (15). Another important role of CS is to increase the expression of TGF-β1 on the extracellular matrix (17) and to promote the synthesis of HA and collagen type II (18)(19). The mechanism of action of HA and CS seems to be complementary, developing a synergistic effect that could explain the results obtained in this study.…”

Section: Discussionmentioning

confidence: 99%

Short-term effect of the combination of hyaluronic acid, chondroitin sulfate, and keratin matrix on early symptomatic knee osteoarthritis

Galluccio¹,

Barskova²,

Cerinic³

2015

Eur J Rheumatol

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Short-term effect of the combination of hyaluronic acid, chondroitin sulfate, and keratin matrix on early symptomatic knee osteoarthritis Abstract Objective: In the last years, symptomatic slow-acting drugs for osteoarthritis (SYSADOA) have been vastly studied and have generated considerable interest among clinicians. SYSADOA are generally used as a ground therapy with the main rationale to reduce the consumption of nonsteroidal anti-inflammatory drugs (NSAIDs) and thus limit the related adverse events. Material and Methods:In this study, we evaluated the short-term effect of an oral combination of hyaluronic acid, chondroitin sulfate, and keratin matrix on early symptomatic knee osteoarthritis. Forty patients were treated for 1 month and were allowed to assume analgesics or NSAIDs if necessary.Results: At 2 months, the mean reduction of the Western Ontario and McMaster Universities Osteoarthritis Index (WOMAC) score was 36% (p<0.001), and the mean reduction of the WOMAC pains score was 40% (p<0.001). Only two patients reported a sporadic need to assume analgesics; no patient reported any side effect during the study period.Conclusion: This data demonstrates that the oral combination of hyaluronic acid, chondroitin sulfate, and keratin matrix is safe, well tolerated, and shows a rapid action reducing pain and improving joint function and stiffness in early symptomatic knee osteoarthritis.

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Section: Discussionmentioning

confidence: 99%

Short-term effect of the combination of hyaluronic acid, chondroitin sulfate, and keratin matrix on early symptomatic knee osteoarthritis

Galluccio¹,

Barskova²,

Cerinic³

2015

Eur J Rheumatol

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“…73 The addition of hyaluronan to cell culture medium increased deposition of type II collagen and glycosaminoglycans, markers of the cartilage phenotype, by chondrocytes encapsulated in alginate hydrogels, 82 but decreased such markers by chondrocytes encapsulated in collagen hydrogels. 83 Other studies have shown that the addition of hyaluronan to alginate hydrogels caused an increase in the expression of type I collagen, a marker of fibrous scar-like cartilage formation. 72 Interestingly, this trend was reversed in the presence of exogenous insulin-like growth factor-1 (IGF-1), suggesting a role for hyaluronan in the modulation of IGF-1 signaling by entrapped chondrocytes.…”

Section: Type Of Polymermentioning

confidence: 99%

Hydrogels for the Repair of Articular Cartilage Defects

Spiller

Maher

Lowman

2011

Tissue Engineering Part B: Reviews

404

312

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“…A previous report indicated that high-molecularweight CSAs did not affect COL II expression, but that high-molecular-weight CSC could enhance it (Nishimoto et al 2005). In our study, low-molecularweight CSAs increased the ratio of COL II/COL I gene expression levels, and the dp-8 CSA was most effective in stimulating aggrecan and COL II gene expression.…”

Section: Discussionmentioning

confidence: 95%

The effects of different molecular weight chondroitin-4-sulfates in chondrocyte pellet culture

Yang

Peng

et al. 2014

Cytotechnology

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For this study, we cultured chondrocyte pellets in Dulbecco's modified Eagle's medium plus a 2 % fetal bovine serum medium, and treated them with 2-to 8-mer oligosaccharides of chondroitin sulfate A to examine the effects of these oligosaccharides on the differentiation and protection of chondrocytes. We found low-molecular-weight CSAs to increase the ratio of the gene expression levels of collagen II/collagen I of chondrocytes from the first day up to 14 days after culture compared with those under a CSA-free medium. Moreover, low-molecularweight CSAs inhibited the expression of matrix metalloproteinases and peptidases, and stimulated an endogenous tissue inhibitor of metalloproteinases. The dp-8 (8-mer) CSA yielded the most effective response among promoting collagen type II protein secretions compared with other groups.

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Effect of chondroitin sulfate and hyaluronic acid on gene expression in a three-dimensional culture of chondrocytes

Cited by 42 publications

References 6 publications

Short-term effect of the combination of hyaluronic acid, chondroitin sulfate, and keratin matrix on early symptomatic knee osteoarthritis

Short-term effect of the combination of hyaluronic acid, chondroitin sulfate, and keratin matrix on early symptomatic knee osteoarthritis

Hydrogels for the Repair of Articular Cartilage Defects

The effects of different molecular weight chondroitin-4-sulfates in chondrocyte pellet culture

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