Effect of Chronic Caffeine Intake on Myocardial Function during Early Growth

Temples, Thomas E.; Geoffray, Dolly J; Nakamoto, Tetsuo; Hartman, Arthur D.; Miller, Heather G.

doi:10.1203/00006450-198704000-00014

Cited by 14 publications

(8 citation statements)

References 32 publications

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“…Although interspecies extrapolation from rat to human has to be made with caution, the present study clearly demonstrates that caffeine intake in early life exerts adverse effects on mitochondria of the heart muscle of the pups and may lead to the malfunction of cardiovascular systems in later life as was shown previously [9].…”

Section: Discussionsupporting

confidence: 78%

“…The present study cannot determine if this anatomical change will last. However, we have shown that various parameters of the cardiovascular function are permanently impaired even if caffeine is removed later, as the previous study has shown [9]. Thus, this study may also suggest that the early disruption of heart muscle could lead to the permanent impairment of cardiovascular function.…”

Section: Discussionsupporting

confidence: 73%

“…After slightly more than a week of sucking the caffeine-containing maternal milk, pups had heart mitochondria that were already swollen and disrupted. Chronic caffeine feeding over a period of 50 days after birth was shown to impair cardiac functioning [9].…”

Section: Discussionmentioning

confidence: 99%

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Effects of Caffeine on Heart Mitochondria in Newborn Rats

Wink

Rossowska²,

Joseph³

et al. 1999

Neonatology

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Caffeine consumption has been implicated in the development of cardiovascular disease. Therefore, in the present study, litters of rats were combined upon birth, and 8 pups were randomly assigned to each dam. Dams with pups were divided into 2 groups: group 1 received a 20% protein diet as a control, and group 2 received the 20% protein diet supplemented with caffeine (4 mg/100 g body weight). Pups from both groups were killed on days 11 and 15. Transmission electron microscopy revealed swollen, disrupted, degenerating mitochondria and intracellular edema in the hearts of rats in the caffeine groups when compared with those of the controls. Plasma Cu concentration was significantly decreased. These results indicate that early exposure to caffeine through maternal milk adversely affects cardiac mitochondria of rat pups and may be associated with decreased plasma Cu levels. It is unclear whether these results apply to the human infant. Interspecies extrapolation from rat to human must be made with caution.

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Section: Discussionsupporting

confidence: 78%

Section: Discussionsupporting

confidence: 73%

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Effects of Caffeine on Heart Mitochondria in Newborn Rats

Wink

Rossowska²,

Joseph³

et al. 1999

Neonatology

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“…The binding of Zn by skeletal muscle SR has shown that Zn competes with Ca for the same binding sites (Carvalho, 1968). Thus, we propose that Zn release from heart tissue as a result of caffeine intake plays a critical role in the functioning of the heart (Temples et a/. 1985).…”

Section: Discussionmentioning

confidence: 78%

Effect of caffeine on zinc absorption and Zn concentration in rat tissue

Rossowska

Nakamoto

1990

Br J Nutr

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The purposes of the present study were to determine whether caffeine has an effect on zinc absorption and tissue levels of Zn. Under anaesthesia, one side of the intestine of female rats was connected to infusion pumps and an infused solution (first caffeine and later Zn solution) was collected from the other side of the intestine using either 300 mm or the whole small intestine to determine Zn absorption. In a further study, different doses of caffeine solution were injected directly into the femoral vein and a saline or Zn solution was infused into the intestine to measure Zn absorption and tissue Zn concentrations. The results consistently showed that the caffeine solution infused into the intestine did not affect intestinal Zn absorption nor was absorption affected by the direct injection of caffeine into the vein. In contrast, injection of different doses of the caffeine solution significantly decreased Zn tissue levels for the heart only. Calcium concentrations in the heart tissue were also decreased, but not magnesium concentrations. Tissue Zn levels recovered immediately on infusion of a Zn solution into the intestine. Caffeine {1,3,7-trimethylxanthine) is contained in beverages such as coffee, tea, cola, other carbonated soft drinks and over-the-counter medications. Because of the widespread use of caffeine in our daily life, it is practically impossible to avoid its intake. A number of studies have described the effects of caffeine on various organs of the body. Chronic caffeine ingestion by growing rats through drinking water is reported to affect the zinc content of the testicle (Friedman et al. 1979) but not of the brain (Haydel et a!. 1986). Zn is known to play an important role in growth and development of animals and humans (Dreosti, 1982), and its absorption from the small intestine greatly increases during the latter part of pregnancy and lactation (Davies & Williams, 1977). On the other hand, Southon et al. (1989) have shown that Zn absorption increases in pregnant rats only at low intakes of Zn. Recently a close interaction was observed between the maternal dietary caffeine intake and Zn content of certain rat tissues such as the brain (Nakamoto et al. 19896) and bone (Nakamoto rt al. 1 9 8 9~) of their offspring. Because there was a decrease in Zn content in these tissues when caffeine was added to the maternal diet, the question raised was whether caffeine could impair intestinal Zn absorption. If impairment of maternal Zn absorption occurs, then it is possible that the tissue levels of Zn in the offspring might be decreased. It is also conceivable that caffeine could directly affect the uptake or release of Zn at the tissue level. Therefore, using adult animal models, we determined whether caffeine affects intestinal Zn absorption and Zn concentrations in various tissues. M A T E R I A L S A N D M E T H O D SAdult female Sprague-Dawley rats (Holtzman strain, Holtzman Co., Madison, WI) (230-250 g) were used for all experiments. The rats were kept in cages in a room with controlled lighting...

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“…Impair-evaporated to 1 mL, were used to determine the Zn content by ment of function of the growing rat heart as a result of chronic atomic absorption s~ectro~hotornetry (model 280; Fisher Scicaffeine intake during the neonatal period has been documented entific Co., Fair Lawn, NJ). Concentrated nitric acid (1 m~) was (7).…”

mentioning

confidence: 99%

Caffeine Decreases Zinc and Metallothionein Levels in Heart of Newborn and Adult Rats

Rossowska

Nakamoto

1992

Pediatr Res

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ABSTRAn. The purpose of the present study is to show (8). Inasmuch as the heart of the newborn rat is growing during that dietary caffeine, heart tissue Zn levels, and metallo-the lactational period, as is shown by the increase of DNA content thionein (MT) concentration are all interrelated in newborn (9), it is conceivable that maternal caffeine intake during this rats. Dams with eight pups in the control group were fed a period could affect Zn tissue levels in the hearts of their offspring 20% protein diet upon delivery, whereas dams in the through suckling milk and/or could alter the composition of the experimental group were fed a 20% protein diet supple-milk, which in turn may cause changes in cardiac Zn levels. The mented with caffeine (2 mg1100 g body weight). Offspring objective of the present study is to determine how chronic were killed at d 22 postbirth and the hearts and livers were caffeine intake by newborn rats affects MT concentration removed to determine the Zn and MT concentrations. through the tissue Zn levels in the heart. We have measured the Hearts of the newborn rats in the caffeine group showed Zn and MT contents of the liver and the heart to compare the decreased Zn levels as well as decreased MT concentration. different effects of caffeine on these organs. MT content of the To explain the observed effects in newborn rats, the rela-liver is well established in the literature, whereas the information tionship between Zn and MT levels was studied in adult on MT content of the heart of newborn rats is limited at the female rats. They were injected intraperitoneally with present time. either ZnClz (20 mg/kg body weight) dissolved in saline solution or ZnClz and caffeine (4 mglkg body weight) over MATERIALS AND METHODS a period of 2 d. Injection of ZnClz into adult female rats resulted in an increase in heart MT levels, whereas injec-Effect of chronic caffeine intake by dams and their newborn tion of caffeine caused decreased Zn levels and MT con-rats. Timed-pregnant rats were obtained from a commercial centration. Current findings indicate that dietary caffeine breeder (Holtzman Co., Madison, WI). At term, litters delivered intake during the lactational period by newborn PUPS within an 8-h period were combined and designated d 1; eight causes a decrease of the heart Zn and MT levels. (Pediatr pups were randomly assigned to each dam. Pup assignment was Res 32: [330][331][332]1992) made without regard to the sex of the pups. The control dams (n = 6) were fed a 20% protein diet a d Abbreviations libitum to determine the mean daily food intake and body weight changes from d 1. The caffeine dams (n = 6) were fed a 20% MT, metallothionein protein diet supplemented with caffeine, in amounts calculated i.p., intraperitoneal to provide 2 mg/100 g body weight to the dams. The dietary SF, supernatant fraction composition of the 20% protein diet has been described (10). The calculated concentration of Zn was 12 mg/kg diet. On d 22, dams and their pups from each group were killed between 0900 and 1100 ...

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Effect of Chronic Caffeine Intake on Myocardial Function during Early Growth

Cited by 14 publications

References 32 publications

Effects of Caffeine on Heart Mitochondria in Newborn Rats

Effects of Caffeine on Heart Mitochondria in Newborn Rats

Effect of caffeine on zinc absorption and Zn concentration in rat tissue

Caffeine Decreases Zinc and Metallothionein Levels in Heart of Newborn and Adult Rats

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