Effect of chronic chlorpyrifos exposure on diaphragmatic muscle contractility and MHC isoforms in adult rats

Sabbouri, Hiba El Khayat El; Hallal, Nancy; Darwiche, Walaa; Gay-Quéheillard, Jérôme; Bach, Véronique; Ramadan, Wiâm; Joumaa, Wissam H.

doi:10.1007/s13530-021-00121-6

Cited by 4 publications

(3 citation statements)

References 66 publications

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“…One of the main consequences of chronic respiratory failure is the progressive increase in tone and strength of muscle contraction, due to the workload exerted on the muscle (33,34). In cases of alteration in the diaphragmatic workload, there is a significant variation in the recruitment of motor units, causing muscle hyperactivation (33). In this sense, it is common to find hypertrophy in muscle fibers (35) and in the junctional postsynaptic compartments (36), as found in the present study.…”

Section: Discussionsupporting

confidence: 77%

“…As one of the main routes of human contamination with GBH is the nasal-oral route, intoxication by this and other organophosphates may be related to respiratory problems, such as cases of respiratory failure (8,31,32). One of the main consequences of chronic respiratory failure is the progressive increase in tone and strength of muscle contraction, due to the workload exerted on the muscle (33,34). In cases of alteration in the diaphragmatic workload, there is a significant variation in the recruitment of motor units, causing muscle hyperactivation (33).…”

Section: Discussionmentioning

confidence: 99%

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Peripubertal Exposure to Glyphosate-Based Herbicides Promotes Histopathological Impairment in the Structure of the Diaphragm Muscle of C57BL/6 Mice

Zazula,

Marca,

de Campos Oliveira

et al. 2024

Muscle Ligaments and Tendons J

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Introduction.Glyphosate is an organophosphate herbicide most used in Latin America, with multisystemic effects, including the respiratory system. In this sense, the objective of this research was to analyze the muscle fibers and neuromuscular junctions (NMJs) of the diaphragm muscle of adult mice exposed to the glyphosate-based herbicide in the peripubertal period. Methods. Twelve male mice were used, divided into a control group (CTL, n = 6), which received water and a glyphosate-based herbicide group (GBH, n = 6), which received 50 mg/Kg/day of Roundup ® , both by gavage from 30 to 60 days. At 150 days, the animals were euthanized, and the diaphragm was collected for analysis of the muscle fibers through hematoxylin-eosin, Masson's trichrome and Picrossirius Red and the NMJs through the nonspecific esterases reaction. Results. Rounded fibers, hypereosinophilic sarcoplasm and enlarged nuclei were found predominantly in GBH. A reduction in body weight, an increase in muscle fiber morphometry, an increase in type III collagen, a decrease in the overlapping of type I and III collagen, and an increase in the area and greater diameter of the NMJs in GBH were observed. Conclusions. Peripubertal exposure to glyphosate-based herbicides showed morphological changes characteristic of muscle degeneration and altered the morphometry of muscle fibers and NMJs of the diaphragm of adult mice.

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Section: Discussionsupporting

confidence: 77%

Section: Discussionmentioning

confidence: 99%

Peripubertal Exposure to Glyphosate-Based Herbicides Promotes Histopathological Impairment in the Structure of the Diaphragm Muscle of C57BL/6 Mice

Zazula,

Marca,

de Campos Oliveira

et al. 2024

Muscle Ligaments and Tendons J

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“…Hallal et al found that long-term exposure to CPF has a negative effect on the movement and contraction of slow and fast skeletal muscles in rats (6). In another study, it was reported that the chronic exposure of adult male rats to CPF is associated with an increase in the contractile function of the diaphragm and fatigue, which can be related to a disturbance in the catabolic and anabolic hormonal balance and/or a possible change in the excitation-contraction coupling mechanism (7). In this regard, the mammalian target of rapamycin mTORC is known as a key regulator in the control of skeletal muscle mass following contraction and hypertrophy caused by mechanical load (8).…”

Section: Introductionmentioning

confidence: 98%

The effect of aerobic training and eugenol supplementation on the PI3K/AKT/mTOR pathway in skeletal muscle of male rats poisoned with chlorpyrifos

Behnam Moghadam,

Matinhomaee,

Azarbayjani

2023

J Shahrekord Univ Med Sci

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Background and aims: Chlorpyrifos (CPF) is an insecticide that is widely used in the world. The purpose of this research was to investigate the effect of 4-week aerobic exercise and eugenol supplementation on the phosphatidylinositol-3-kinases/protein kinase/mammalian target of rapamycin (Pi3K/AKT/mTOR) pathway on the skeletal muscle of male rats poisoned with CPF. Methods: Overall, 12-week-old female rats were used in this experimental research. The rats were randomly divided into 8 groups (8 rats in each group), including healthy control, toxic control, poison solvent, corn oil solvent, poisoned+eugenol, poisoned+aerobic exercise, and poisoned+aerobic exercise+eugenol. Moderate training was in the range of 50-60% VO2 max, including 5 training sessions per week (treadmill). Poisoning was performed with CPF poison with a dose of 3 mg/kg, and the dose of eugenol was determined to be 250 mg/kg. Results: There was no significant difference between the groups in terms of mTOR and AKT expression (P=0.369, P=0.59). However, the expression of PI3k in the poisoned control group was lower than that in the healthy control group (P=0.049). In addition, the expression of PI3k was higher in the poisoned+eugenol+exercise group compared to the poisoned control group (P=0.009). The corn solvent group also had a higher PI3k expression in comparison to the poisoned control group (P=0.025). Finally, there was no significant difference among the other groups. Conclusion: In general, 4 weeks of CPF poisoning caused a significant decrease in PI3K, but it did not have a significant effect on AKT and mTOR. Based on the finding, 4 aerobic exercises and eugenol consumption could significantly increase in PI3K, while it had no significant effect on AKT and mTOR.

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Can animals tune tissue mechanics in response to changing environments caused by anthropogenic impacts?

James

Seebacher

Tallis

2023

Journal of Experimental Biology

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Anthropogenic climate change and pollution are impacting environments across the globe. This Review summarises the potential impact of such anthropogenic effects on animal tissue mechanics, given the consequences for animal locomotor performance and behaviour. More specifically, in light of current literature, this Review focuses on evaluating the acute and chronic effects of temperature on the mechanical function of muscle tissues. For ectotherms, maximal muscle performance typically occurs at temperatures approximating the natural environment of the species. However, species vary in their ability to acclimate to chronic changes in temperature, which is likely to have longer-term effects on species range. Some species undergo periods of dormancy to avoid extreme temperature or drought. Whilst the skeletal muscle of such species generally appears to be adapted to minimise muscle atrophy and maintain performance for emergence from dormancy, the increased occurrence of extreme climatic conditions may reduce the survival of individuals in such environments. This Review also considers the likely impact of anthropogenic pollutants, such as hormones and heavy metals, on animal tissue mechanics, noting the relative paucity of literature directly investigating this key area. Future work needs to determine the direct effects of anthropogenic environmental changes on animal tissues and related changes in locomotor performance and behaviour, including accounting for currently unknown interactions between environmental factors, e.g. temperature and pollutants.

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Effect of chronic chlorpyrifos exposure on diaphragmatic muscle contractility and MHC isoforms in adult rats

Cited by 4 publications

References 66 publications

Peripubertal Exposure to Glyphosate-Based Herbicides Promotes Histopathological Impairment in the Structure of the Diaphragm Muscle of C57BL/6 Mice

Peripubertal Exposure to Glyphosate-Based Herbicides Promotes Histopathological Impairment in the Structure of the Diaphragm Muscle of C57BL/6 Mice

The effect of aerobic training and eugenol supplementation on the PI3K/AKT/mTOR pathway in skeletal muscle of male rats poisoned with chlorpyrifos

Can animals tune tissue mechanics in response to changing environments caused by anthropogenic impacts?

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