Effect of Chronic Moderate Caloric Restriction on the Reproductive Function in Aged Male Wistar Rats

Jesús, Pablo López de; Arenas‐Ríos, Edith; Ruíz-Ramos, Mirna; Flores-Alonso, Juan Carlos; Mendoza-Núñez, Vı́ctor Manuel; Arrieta‐Cruz, Isabel; Arteaga-Silva, Marcela

doi:10.3390/nu14061256

Cited by 6 publications

(5 citation statements)

References 56 publications

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“…Comparatively, the mice in previous studies were around 4 – 6 months of age on their last MSB test (David, Wyrosdic, & Park, 2022; David, Wyrosdic, Wan, et al ., 2022). This is a potential confound as ageing is known to impair MSB (Wilson et al ., 2018; Jesús et al ., 2022). Future studies could independently test these variables (i.e social isolation, social exposure, and ageing) to determine how each may contribute to the altered percentage of SI persistent maters.…”

Section: Discussionmentioning

confidence: 99%

Neuron- and microglia-specific immunoexpression in steroid-independent male sexual behaviour in castrated B6D2F1 male mice

Chiang

Lipkin

McCusker

et al. 2022

Preprint

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Sexual behaviour is the sine qua non for procreation for several species and is traditionally viewed to be regulated by sex steroid hormones. However, several species exhibit steroid-independent sexual behaviour, and its molecular understanding is only beginning to be uncovered. We do not yet have a cell-specific understanding of the roles non-neuronal cells play in this phenomenon. Forty B6D2F1 hybrid male mice underwent orchidectomy and some of them exhibited consistent ejaculatory reflexes at least 62 weeks post-orchidectomy over 11 sexual behaviour tests (>180 minutes each), defined as steroid-independent (SI) maters (21.80%), and those that do not, defined as SI non-maters (25%). Using flow cytometry, we compared the preoptic area immunoexpression in NeuN+ neurons and Iba1+ microglia between SI maters and SI non-maters (n = 6 per group). We found neuronal immunoexpression up-regulated for amyloid precursor protein and androgen receptor, as well as down-regulated for glucocorticoid receptor. In conjunction, microglial immunoexpression of amyloid precursor protein up-regulated. These data suggest there are cell-specific immunoexpression changes including a role of non-neuronal cells in steroid-independent male sexual behaviour.

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Section: Discussionmentioning

confidence: 99%

Neuron- and microglia-specific immunoexpression in steroid-independent male sexual behaviour in castrated B6D2F1 male mice

Chiang

Lipkin

McCusker

et al. 2022

Preprint

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“…High-calorie diets generally have negative effects on male fertility (e.g. Rato et al (2014)), whereas the effects of dietary restriction on male fertility and reproduction vary: Dietary restriction had a negative effect on sperm count, sperm motility and capacitation ability in young 2-month-old Sprague-Dawley rats (Rattus norvegicus) (Li et al, 2021), but had no effects on ejaculate quality in 2.5-month-old rats that were also exposed to heat stress (Aydilek et al, 2015) and led to a positive effect on ejaculate quality in aged 12-month-old Wistar rats (Jesús et al, 2022).…”

Section: Introductionmentioning

confidence: 99%

“…Rato et al. (2014)), whereas the effects of dietary restriction on male fertility and reproduction vary: Dietary restriction had a negative effect on sperm count, sperm motility and capacitation ability in young 2‐month‐old Sprague–Dawley rats ( Rattus norvegicus ) (Li et al., 2021), but had no effects on ejaculate quality in 2.5‐month‐old rats that were also exposed to heat stress (Aydilek et al., 2015) and led to a positive effect on ejaculate quality in aged 12‐month‐old Wistar rats (Jesús et al., 2022). A randomised trial of dietary restriction in obese men showed an increase in sperm count and sperm concentration in the trial group under caloric restriction (Andersen et al., 2022), whereas a systematic meta‐analysis on the impact of dietary manipulation on sperm has reported that food reduction led to alterations in ejaculate traits in mammals, arthropods and fish, but the extension of the effects varied across taxa (Macartney et al., 2019).…”

Section: Introductionmentioning

confidence: 99%

Paternal starvation affects metabolic gene expression during zebrafish offspring development and lifelong fitness

Jimenez‐Gonzalez,

Ansaloni,

Nebendahl

et al. 2024

Molecular Ecology

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Dietary restriction in the form of fasting is a putative key to a healthier and longer life, but these benefits may come at a trade‐off with reproductive fitness and may affect the following generation(s). The potential inter‐ and transgenerational effects of long‐term fasting and starvation are particularly poorly understood in vertebrates when they originate from the paternal line. We utilised the externally fertilising zebrafish amenable to a split‐egg clutch design to explore the male‐specific effects of fasting/starvation on fertility and fitness of offspring independently of maternal contribution. Eighteen days of fasting resulted in reduced fertility in exposed males. While average offspring survival was not affected, we detected increased larval growth rate in F1 offspring from starved males and more malformed embryos at 24 h post‐fertilisation in F2 offspring produced by F1 offspring from starved males. Comparing the transcriptomes of F1 embryos sired by starved and fed fathers revealed robust and reproducible increased expression of muscle composition genes but lower expression of lipid metabolism and lysosome genes in embryos from starved fathers. A large proportion of these genes showed enrichment in the yolk syncytial layer suggesting gene regulatory responses associated with metabolism of nutrients through paternal effects on extra‐embryonic tissues which are loaded with maternal factors. We compared the embryo transcriptomes to published adult transcriptome datasets and found comparable repressive effects of starvation on metabolism‐associated genes. These similarities suggest a physiologically relevant, directed and potentially adaptive response transmitted by the father, independently from the offspring's nutritional state, which was defined by the mother.

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“…Dietary restriction had a negative effect on sperm count and sperm motility and capacitation ability in young two-months old Sprague Dawley rats (Li et al, 2021). However, it had no effects on 2.5-months old Sprague Dawley rats that were also exposed to heat stress (Aydilek et al, 2015) or led to a positive effect in aged 12-months old Wistar rats (Jesús et al, 2022). A randomised trial of dietary restriction in obese men showed an increase in sperm count and sperm concentration in the trial group under caloric restriction (Andersen et al, 2022).…”

Section: Introductionmentioning

confidence: 99%

Paternal starvation affects metabolic gene expression during zebrafish offspring development and life-long fitness

Jimenez-Gonzalez,

Ansaloni,

Nebendahl

et al. 2023

Preprint

View full text Add to dashboard Cite

Dietary restriction is a putative key to a healthier and longer life, but these benefits may come at a trade-off with reproductive fitness and may affect the following generation(s). The potential inter- and transgenerational effects of starvation are particularly poorly understood in vertebrates when they originate from the paternal line. We utilised the externally fertilising zebrafish amenable to a split-egg clutch design to explore the male-specific effects of starvation on fertility and fitness of offspring independently of maternal contribution. Eighteen days of fasting resulted in reduced fertility in exposed males. While average offspring survival was not affected, we detected higher larval growth in offspring from starved males and increased malformation rates at 24 hours post fertilisation in the F2 embryos produced by the offspring of the starved males. The transcriptome analysis of embryos from starved and fed fathers revealed robust and reproducible induction of muscle composition genes and a contrasting repressive effect on lipid metabolism and lysosome genes. A large proportion of these genes showed enrichment in the yolk syncytial layer suggesting gene regulatory responses associated with metabolism of nutrients through paternal impact on extra embryonic tissues which are loaded with maternally deposited factors. We compared the embryo transcriptome to adult transcriptome datasets and demonstrated comparable repressive effects on metabolism-associated genes. These similarities suggest a physiologically relevant, directed and potentially adaptive response transmitted by the father, independently from the offspring's nutritional state, which was defined by the mother.

show abstract

Effect of Chronic Moderate Caloric Restriction on the Reproductive Function in Aged Male Wistar Rats

Cited by 6 publications

References 56 publications

Neuron- and microglia-specific immunoexpression in steroid-independent male sexual behaviour in castrated B6D2F1 male mice

Neuron- and microglia-specific immunoexpression in steroid-independent male sexual behaviour in castrated B6D2F1 male mice

Paternal starvation affects metabolic gene expression during zebrafish offspring development and lifelong fitness

Paternal starvation affects metabolic gene expression during zebrafish offspring development and life-long fitness

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