Effect of chronic stress in newborn rats on the progression of ligature-induced-periodontitis in adulthood

Abstract

PURPOSE:To investigate the effect chronic stress in newborn rats on the progression of ligature-induced-periodontitis in adulthood. METHODS:Ten couples of adult Wistar rats were selected for mating. After birth, the female and their respective offsprings were allocated into two groups. Ligature Group (LG): offsprings were only watched during breast-feeding; Stress-ligature Group (SLG): after 24h of live birth, the offsprings were moved away from their mothers every day for four hours during breast-feeding for 20 days; in both approaches, after reaching ± 250g, ten rats were included in the groups. Periodontal disease was induced by a silk suture placed around the maxillary right second molar. The left side was used as control. After 15 days, the animals were subjected to euthanasia, maxillary bones were removed and stored in 10% formaldehyde. After 48h, radiographs were taken and revealed and were used for bone destruction analysis. Examiner was blind and calibrated for measurements. RESULTS:Stress-ligature group presented higher bone loss values in relation to ligature group (p<0.05). CONCLUSION:Exposure to chronic stress imposed on offsprings produced a greater progression of bone loss induced during adulthood.

Effects of stress hormones on the production of volatile sulfur compounds by periodontopathogenic bacteria

“…4,7,8,9,10 However, the mechanisms involved in this process are still not clear. Potentially pathogenic microorganisms can recognize stress hormones, a fact that has led to the development of the concept of "microbial endocrinology."…”

Chronic stress associated to contraceptives use on the progression of ligature-induced periodontitis in rats

“…Specific lineages of animals were chosen to test the study hypothesis because it seems that inbred rats are only minimally susceptible to periodontitis 27 and do not experience substantial destruction of periodontal structures.…”

“…After 15 days, the effects of disease progression are likely to be identified. However, Chronic stress associated to contraceptives… 295 after 30 days, the responses begin to differentiate according to stress modulation 15,27 ; furthermore, this length of time is a reasonable period within which to detect hormonal changes 28 .…”

Enfermedad periodontal y disfunción eréctil

“…Sin embargo, las disfunciones eréctiles son también de naturaleza psicológica 40 . El estrés, a su vez, también es un indicador de riesgo de enfermedad periodontal 41,42 . Evaluar el efecto de posibles variables confundentes como diabetes, obesidad, estrés cró-nico y tabaquismo y ajustarlas con modelo de regresión logística multinivel es necesario para aclarar la asociación, así como también estudios longitudinales mediante ensayos clínicos aleatorizados controlados, para evaluar el efecto de la terapia periodontal en el manejo de la DE.…”