Effect of cigarette smoking on human oviductal ciliation and ciliogenesis

Pier, Bruce; Kazanjian, Avedis; Gillette, Laurie; Strenge, Karen; Burney, Richard O.

doi:10.1016/j.fertnstert.2012.08.041

Cited by 10 publications

(3 citation statements)

References 25 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Indeed the up-regulated cell survival gene BCL2 appeared to be exclusively expressed in the non-ciliated cells of the epithelium and could be accounted for by a relative decrease in ciliated cell numbers. However, a recent retrospective cohort study of the effects of cigarette smoke on epithelial ciliation and ciliogenesis in human FT did not reveal any significant changes in ciliated cell numbers or transcription factors involved in ciliogenesis [27].…”

Section: Discussionmentioning

confidence: 88%

The Association between Smoking and Ectopic Pregnancy: Why Nicotine Is BAD for Your Fallopian Tube

et al. 2014

View full text Add to dashboard Cite

Epidemiological studies have shown that cigarette smoking is a major risk factor for tubal ectopic pregnancy but the reason for this remains unclear. Here, we set out to determine the effect of smoking on Fallopian tube gene expression. An oviductal epithelial cell line (OE-E6/E7) and explants of human Fallopian tubes from non-pregnant women (n = 6) were exposed to physiologically relevant concentrations of cotinine, the principle metabolite of nicotine, and changes in gene expression analyzed using the Illumina Human HT-12 array. Cotinine sensitive genes identified through this process were then localized and quantified in Fallopian tube biopsies from non-pregnant smokers (n = 10) and non-smokers (n = 11) using immunohistochemistry and TaqMan RT-PCR. The principle cotinine induced change in gene expression detected by the array analysis in both explants and the cell line was significant down regulation (P<0.05) of the pro-apoptotic gene BAD. We therefore assessed the effect of smoking on cell turnover in retrospectively collected human samples. Consistent with the array data, smoking was associated with decreased levels of BAD transcript (P<0.01) and increased levels of BCL2 transcript (P<0.05) in Fallopian tube biopsies. BAD and BCL2 specific immunolabelling was localized to Fallopian tube epithelium. Although no other significant differences in levels of apoptosis or cell cycle associated proteins were observed, smoking was associated with significant changes in the morphology of the Fallopian tube epithelium (P<0.05). These results suggest that smoking may alter tubal epithelial cell turnover and is associated with structural, as well as functional, changes that may contribute to the development of ectopic pregnancy.

show abstract

Section: Discussionmentioning

confidence: 88%

The Association between Smoking and Ectopic Pregnancy: Why Nicotine Is BAD for Your Fallopian Tube

et al. 2014

View full text Add to dashboard Cite

show abstract

“…FOXJ1 and PAX8 are commonly used as markers of ciliated and secretory cells, respectively (Pier et al 2013;Lau et al 2014). Interestingly, the cyclic changes of the proportion of FOXJ1-positive cells (ciliated cells) were coincident with that of Ki67-positive cells.…”

Section: Discussionmentioning

confidence: 99%

Remodeling of bovine oviductal epithelium by mitosis of secretory cells

et al. 2016

View full text Add to dashboard Cite

Two types of oviductal epithelial cells, secretory and ciliated, play crucial roles in the first days after fertilization in mammals. Secretory cells produce various molecules promoting embryo development, while ciliated cells facilitate transport of oocytes and zygotes by ciliary beating. The proportions of the two cell types change during the estrous cycle. The proportion of ciliated cells on the oviductal luminal surface is abundant at the follicular phase, whereas the proportion of secretory cells gradually increases with the formation of the corpus luteum. In the present study, we hypothesize that the proportions of ciliated and secretory epithelial cells are regulated by mitosis. The proportion of the cells being positive for FOXJ1 (a ciliated cell marker) or Ki67 (a mitosis marker) in epithelial cells during the estrous cycle were immunohistochemically examined. Ki67 and FOXJ1 or PAX8 (a secretory cell marker), were double-stained to clarify which types of epithelial cells undergo mitosis. In the ampulla, the percentage of FOXJ1-positive cells was highest at the day of ovulation (Day 0) and decreased by about 50 % by Days 8-12, while in the isthmus it did not change during the estrous cycle. The proportion of Ki67-positive cells was highest at around the time of ovulation in both the ampulla and isthmus. All the Ki67-positive cells were PAX8-positive and FOXJ1-negative in both the ampulla and isthmus. These findings suggest that epithelial remodeling, which is regulated by differentiation and/or proliferation of secretory cells of the oviduct, provides the optimal environment for gamete transport, fertilization and embryonic development.

show abstract

“…This effect has been attributed to detachments of the connections linking the ciliated epithelium and the oocyte [104]. Moreover, though some authors have suggested that chronic tobacco exposure does not have a measurable effect on the number of ciliated cells or expression of ciliogenesis-specific transcription factors studied in human fallopian tube specimens obtained at the time of tubal sterilization [105], others have demonstrated a reduction in the number of ciliated cells and ciliogenesis in the tubal epithelium following exposure to tobacco smoke [106].…”

Section: Smokingmentioning

confidence: 99%

Tubal transport of gametes and embryos: a review of physiology and pathophysiology

Ezzati

Djahanbakhch

Arian³

et al. 2014

J Assist Reprod Genet

106

View full text Add to dashboard Cite

With the advent of assisted reproductive technology in the past three decades, the clinical importance of fallopian tubes has been relatively overlooked. However, successful spontaneous conception requires normal function of the tube to provide not only a conduit for the gametes to convene and embryo to reach the uterine cavity, but also a physiologically optimized environment for fertilization and early embryonic development. In this review, after a brief description of normal human tubal anatomy and histology, we will discuss tubal transport and its principal effectors, including ciliary motion, muscular contractility and tubal fluid. Furthermore, we will discuss the ciliary ultrastructure and regulation of ciliary beat frequency by ovarian steroids, follicular fluid, angiotensin system, autonomic nervous system and other factors such as adrenomedullin and prostaglandins. In the last section, we describe the adverse impact of various pathological conditions, such as endometriosis, infection and smoking on tubal function and ciliary motility.

show abstract

Effect of cigarette smoking on human oviductal ciliation and ciliogenesis

Cited by 10 publications

References 25 publications

The Association between Smoking and Ectopic Pregnancy: Why Nicotine Is BAD for Your Fallopian Tube

The Association between Smoking and Ectopic Pregnancy: Why Nicotine Is BAD for Your Fallopian Tube

Remodeling of bovine oviductal epithelium by mitosis of secretory cells

Tubal transport of gametes and embryos: a review of physiology and pathophysiology

Contact Info

Product

Resources

About