Effect of cigarette smoking on DNA damage of human cumulus cells analyzed by comet assay

Sinkó, Ildikó; Mórocz, Mónika; Zádori, János; Kokavszky, Katalin; Raskó, István

doi:10.1016/j.reprotox.2004.12.007

Cited by 47 publications

(39 citation statements)

References 40 publications

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“…In previous studies in which the effects of cigarette smoke condensate/cigarette smoke on DNA strand breaks were investigated by the comet assay, only DNA single-strand breaks were detected (18,35,36). The interaction of CSC on DNA strand breaks in HPV-transformed cervical cells has not been reported previously.…”

Section: Discussionmentioning

confidence: 92%

Cigarette smoke-induced DNA damage and repair detected by the comet assay in HPV-transformed cervical cells

Moktar

Ravoori²,

Vadhanam³

et al. 2009

Int J Oncol

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Abstract. Human papillomavirus (HPV) is the causative factor in the development and progression of cervical cancers in >97% of the cases, although insufficient. Epidemiological studies suggest an elevated risk of cervical cancer for cigarette smokers; therefore, we examined cigarette smoke-induced DNA damage and repair in HPV16-transformed human ectocervical cells (ECT1/E6 E7). Cells were treated with cigarette smoke condensate (CSC) for 72 h to assess the formation of single-and double-strand DNA breaks, measured by alkaline and neutral single cell gel electrophoresis assays, respectively. The mean tail length of cells with single-strand breaks was increased by 1.8-, 2.7-and 3.7-fold (p<0.001) after treatment with 4, 8 and 12 μg/ml CSC, respectively. The tail length with double-strand breaks was also increased dose-dependently. These results were further supported by measurement of the mean tail moment: the increase in both single-and double-strand breaks were much more pronounced with increasing concentration of CSC, by up to 23.5-fold (p<0.0001 for both assays). To examine the DNA repair, cells were treated with CSC for 72 h, followed by CSC withdrawal and re-incubation of the cells with fresh medium for 24, 48, or 72 h. Both single-and double-strand DNA breaks were removed during the initial 24 h but no further removal of the damage was observed. Up to 80% of residual single-and double-strand DNA breaks (p<0.05) were found to persist at all CSC concentrations examined. Ellagic acid, a known antioxidant and free-radical scavenger, was found to significantly inhibit DNA breaks induced by CSC. Thus, free radicals may be a plausible source of CSCinduced DNA damage. These data show that CSC-mediated DNA strand breaks are highly persistent, and suggest that persistence of cigarette smoke-associated DNA damage in the presence of HPV infection may lead to increased mutations in cervical cells and ultimately higher cancer risk.

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Section: Discussionmentioning

confidence: 92%

Cigarette smoke-induced DNA damage and repair detected by the comet assay in HPV-transformed cervical cells

Moktar

Ravoori²,

Vadhanam³

et al. 2009

Int J Oncol

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“…This approach showed that oocyte maturation was inhibited in smokers, who produced significantly more diploid oocytes than did nonsmokers (Zenzes et al, 1995). In addition, cumulus cells collected during IVF procedures showed more DNA damage in smokers than did nonsmokers (Sinko et al, 2005). Zona pellucida thickness has also been found to be thicker around oocytes from active and passive smokers (Shiloh et al, 2004).…”

Section: Ovarian Follicular Explantsmentioning

confidence: 95%

In vitro assessment of reproductive toxicity of tobacco smoke and its constituents

Talbot

2008

Birth Defects Research Pt C

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Epidemiological studies have repeatedly shown that reproductive processes in pregnant women are adversely affected by exposure to cigarette smoke. The potential reproductive targets of smoke during pregnancy include the ovaries, oviducts, uterus, placenta, umbilical cord, and embryo/fetus. In vitro methods for studying the effects of smoke and its individual components have been developed and applied to each of these reproductive targets. In vitro assays have been useful in determining the biological processes that are affected in the reproductive organs and in identifying the cellular and molecular targets of smoke in each organ. In vitro methods have also been used to study the mechanism of action of smoke constituents, such as nicotine, on specific processes in reproductive organs and to screen smoke solutions to identify the molecules that affect reproduction. In general, data collected in vitro have confirmed, extended, and helped explain what has been learned from epidemiological studies. This review summarizes some of the in vitro assays that have been used to study cigarette smoke's effect on the nonpregnant and pregnant female reproductive tract and spotlights examples of their applications.

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“…Genotoxicity of various industrial chemicals, agrochemicals, pharmaceuticals and pollutants can be assessed with comet assay [25]. This assay can detect DNA damage in different cell types such as in skeletal muscle cells [26], cumulus cells [27], eosinophils [28] and ovarian cells [29]. It has also been used to detect DNA damage in blood of Polish copper smelter workers occupationally exposed to inorganic arsenic [30].…”

Section: Introductionmentioning

confidence: 99%

Assessment of Arsenic Induced DNA Fragmentation by using Comet Assay

Mahjabeen¹

2016

J Carcinog Mutagen

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Arsenic is metalloid present in measureable quantity in air, water, and soil through natural and anthropogenic sources. It is neurotoxic, hepatotoxic and genotoxic effects, variety of health problems have been associated to arsenic exposure. This review was designed to investigate the possible association between arsenic exposure and DNA damage in animals and humans using comet assay. Total 28 studies were selected for measuring DNA damage by comet assay. Trend of significance in tail length and tail moment was observed using regression analysis. Due to limited number of studies available the regression analysis was non-significant. Individually each study suggested a significant increase in tail moment and tail length in a dose and time-dependent manner. Overall trend observed in this review is the positive association between arsenic exposure either experimentally or occupationally and DNA damage. This initial effort may provide future guideline for the assessment of DNA fragmentation using comet assay.

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Effect of cigarette smoking on DNA damage of human cumulus cells analyzed by comet assay

Cited by 47 publications

References 40 publications

Cigarette smoke-induced DNA damage and repair detected by the comet assay in HPV-transformed cervical cells

Cigarette smoke-induced DNA damage and repair detected by the comet assay in HPV-transformed cervical cells

In vitro assessment of reproductive toxicity of tobacco smoke and its constituents

Assessment of Arsenic Induced DNA Fragmentation by using Comet Assay

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