Effect of ciglitazone on adipogenic transdifferentiation of bovine
                    skeletal muscle satellite cells

Zhang, Junfang; Li, Qiang; Yan, Yan; Sun, Bin; Wang, Ying; Tang, Lin; Wang, Enze; Yu, Jia; Nogoy, Kim Margarette Corpuz; Li, Xiangzi; Choi, Seong‐Ho

doi:10.5187/jast.2021.e87

Cited by 6 publications

(5 citation statements)

References 34 publications

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“…Therefore, oil red O staining solution adsorbs dyes from the solvent to stain lipid droplets, and positive staining results for fat are orange to red [38]. In this study, the effects observed after oil red O staining of HepG2 cells were consistent with the above predictions and showed that luteolin, naringin, and apigenin alleviate oleic acid-induced lipid accumulation.…”

Section: Discussionsupporting

confidence: 87%

Hypolipidemic activity and mechanism of active components of Dracocephalum moldavica L. tea

Li,

Zhang

et al. 2024

Food Science and Human Wellness

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Section: Discussionsupporting

confidence: 87%

Hypolipidemic activity and mechanism of active components of Dracocephalum moldavica L. tea

Li,

Zhang

et al. 2024

Food Science and Human Wellness

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“…Indeed, thiazolidine derivatives act as PPARγ agonists and effectively increase the adiponectin concentration and adipogenic gene expression [28,78]. Unsaturated fatty acids, eicosanoids and non-steroidal anti-inflammatory drugs function in a similar manner [79]. Further metabolic pathways, e.g., the IGF transport and uptake of IGF-binding proteins pathway, type I diabetes mellitus signaling, lipid and atherosclerosis, C-type leptin receptor signaling, estrogen-dependent nuclear events and extranuclear signaling and RETN signaling, encoding resistin, are dysregulated in HS, as shown in the present review.…”

Section: Hs Pathogenesismentioning

confidence: 99%

Hidradenitis Suppurativa and Comorbid Disorder Biomarkers, Druggable Genes, New Drugs and Drug Repurposing—A Molecular Meta-Analysis

Zouboulis

2021

Pharmaceutics

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Chronic inflammation and dysregulated epithelial differentiation, especially of hair follicle keratinocytes, have been suggested as the major pathogenetic pathways of hidradenitis suppurativa/acne inversa (HS). On the other hand, obesity and metabolic syndrome have additionally been considered as an important risk factor. With adalimumab, a drug has already been approved and numerous other compounds are in advanced-stage clinical studies. A systematic review was conducted to detect and corroborate HS pathogenetic mechanisms at the molecular level and identify HS molecular markers. The obtained data were used to confirm studied and off-label administered drugs and to identify additional compounds for drug repurposing. A robust, strongly associated group of HS biomarkers was detected. The triad of HS pathogenesis, namely upregulated inflammation, altered epithelial differentiation and dysregulated metabolism/hormone signaling was confirmed, the molecular association of HS with certain comorbid disorders, such as inflammatory bowel disease, arthritis, type I diabetes mellitus and lipids/atherosclerosis/adipogenesis was verified and common biomarkers were identified. The molecular suitability of compounds in clinical studies was confirmed and 31 potential HS repurposing drugs, among them 10 drugs already launched for other disorders, were detected. This systematic review provides evidence for the importance of molecular studies to advance the knowledge regarding pathogenesis, future treatment and biomarker-supported clinical course follow-up in HS.

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“…BSCs were isolated from the semimembranosus muscle of 8-day-old Yanbian cattle using pronase (10165921001, Roche, Basel, Switzerland) digestion, as described previously [ 14 ]. Details of the BSC culture were also been described in our previous studies [ 14 , 15 ]. Briefly, the BSCs were incubated in DMEM (Gibco, Thermo Fisher Scientific, Waltham, MA, USA) containing 10% fetal bovine serum (FBS) (Gibco, Thermo Fisher Scientific, Waltham, MA, USA) in a humidified incubator at 37 °C and 5% CO 2 .…”

Section: Methodsmentioning

confidence: 99%

Overexpression of DGAT2 Stimulates Lipid Droplet Formation and Triacylglycerol Accumulation in Bovine Satellite Cells

Zhang

et al. 2022

Animals

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Intramuscular fat (IMF) is closely related to the tenderness, juiciness, and flavor of beef, and is an important indicator for beef quality assessment internationally. The main components of skeletal intramuscular fat (IMF) are phospholipids and triacylglycerols (TAG), and the final step of TAG biosynthesis is catalyzed by diacylglycerol acyltransferase 2 (DGAT2). To explore the effect of DGAT2 on the differentiation of bovine muscle satellite cells (BSCs) and its role in the signaling pathway related to lipid metabolism, the adenovirus overexpression and interference vector of the DGAT2 gene was constructed in this study, and the overexpression adenovirus Ad-DGAT2 and interfering adenovirus sh-DGAT2 were used to infect BSCs. Overexpression of DGAT2 resulted in a significant increase in the contents of TAG and ADP, and the mRNA and protein expression levels of PPARγ, C/EBPα, and SREBF1 (p < 0.05). Interfering with the expression of DGAT2 reduced the intracellular TAG content and lipid droplet accumulation. Furthermore, the mRNA and protein expression levels of PPARγ, C/EBPα, and SREBF1 (p < 0.05) were significantly downregulated. Transcriptome sequencing showed that a total of 598 differentially expressed genes (DEGs) were screened in BSCs infected with Ad-DGAT2, and these DEGs included 292 upregulated genes and 306 downregulated genes. A total of 49 DEGs were screened in BSCs infected with sh-DGAT2, and these DEGs included 25 upregulated and 24 downregulated genes. KEGG enrichment analysis showed that the DEGs, after overexpression of DGAT2, were mainly enriched in the PPAR signaling pathway, and the fat digestion and absorption, glycerophospholipid metabolism, fatty acid biosynthesis, and AMPK signaling pathways. The DEGs obtained after interfering with DGAT2 were mainly enriched in the metabolic pathways, such as the PPAR signaling pathway and PI3K/AKT signaling pathway. In summary, our study demonstrated that the lipid droplet formation, TAG accumulation, and adipogenic gene expression in BSCs overexpressing DGAT2 were higher than those in the control cells. These results highlight the important role of DGAT2 in regulating BSCs during adipogenic transdifferentiation and underscore the complexity of intramuscular adipogenesis.

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Effect of ciglitazone on adipogenic transdifferentiation of bovine skeletal muscle satellite cells

Abstract: Funding sourcesState funding sources (grants, funding sources, equipment, and supplies). Include name and number of grant if available.

Cited by 6 publications

References 34 publications

Hypolipidemic activity and mechanism of active components of Dracocephalum moldavica L. tea

Hypolipidemic activity and mechanism of active components of Dracocephalum moldavica L. tea

Hidradenitis Suppurativa and Comorbid Disorder Biomarkers, Druggable Genes, New Drugs and Drug Repurposing—A Molecular Meta-Analysis

Overexpression of DGAT2 Stimulates Lipid Droplet Formation and Triacylglycerol Accumulation in Bovine Satellite Cells

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