Effect of citric acid on toxicity of graphene towards freshwater algae (Chlorella pyrenoidosa)

Guo, Chuntong; Song, Pingping; Cao, Xuesong

doi:10.2991/emcpe-16.2016.70

Cited by 3 publications

(2 citation statements)

References 13 publications

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“…The data obtained can be explained by the fact that the use of citric acid in working concentrations goes beyond the boundaries of the chlorella optimum zone. In other studies, it was shown that citric acid plays the role of a protector (at concentrations of 5, 10, 20 mg/l) during the action of graphene on the Chlorella pyrenoidesa cell culture [27]. The role of citric acid in the protection of higher plants during copper intoxication was also noted [28].…”

Section: Resultsmentioning

confidence: 87%

Cytotoxicity of some preservatives for cultureChlorella VulgarisGKO strain

Azhikina,

Makhnin,

Berenev

et al. 2023

BIO Web Conf.

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One of the ways to preserve the culture of microalgae for a long time is conservation. All modern methods of preservation and long-term storage of microorganism cultures are based on the transfer of cells to a state of anabiosis, in which metabolic processes are either completely or partially stopped. One of the mechanisms that are realized during the transition to the anabiotic state is a change in cell permeability. This can be achieved by modifying the culture medium. Thus, the search for preservatives that contribute to the long-term preservation of biomass is very relevant. The primary condition for the use of any preservative or a new component of the culture medium should be the absence of a toxic effect on the cell culture. Goal of the work: to determine the cytotoxic effect of some preservatives for the cell culture of Chlorella vulgaris GKO strain. The cytotoxicity of ascorbic acid, lactic acid, citric acid, acetic acid, sodium chloride and urotropin was analyzed for the cell culture of Chlorella vulgaris strain GKO. The following parameters were determined: pH of Chlorella vulgaris suspension with preservative; Total number of cells, MM/ml; The ratio of dead cells to the total number of cells,%; Specific growth/death rate; The optical density of the suspension; The difference in the average optical density,%; Cell size (diameter), µm pH of suspension of Chlorella vulgaris with preservative; Total number of cells, MM/ml; The ratio of dead cells to the total number of cells,%; Specific growth/death rate; The optical density of the suspension; The difference in the average optical density,%; Cell size (diameter), µm pH of suspension of Chlorella vulgaris with preservative; Total number of cells, MM/ml; The ratio of dead cells to the total number of cells,%; Specific growth/death rate; The optical density of the suspension; The difference in the average optical density,%; Cell size (diameter), µm

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Section: Resultsmentioning

confidence: 87%

Cytotoxicity of some preservatives for cultureChlorella VulgarisGKO strain

Azhikina,

Makhnin,

Berenev

et al. 2023

BIO Web Conf.

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“…Wang et al (2016) also reported that graphene inhibited growth in Scenedesmus obliquus in a concentrationdependent manner. Guo et al (2016) observed dose-dependent toxicity of graphene in another species, Chlorella pyrenoidosa.…”

Section: Introductionmentioning

confidence: 90%

Comparative ecotoxicity of graphene, functionalized multiwalled CNT and their mixture in freshwater microalgae, Scenedesmus obliquus: Analysing the role of oxidative stress

Das

Giri

Wadhwa

et al. 2023

Preprint

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Due to their remarkable properties, the applications of carbon-based nanomaterials (CNMs) such as graphene and functionalized multi-walled carbon nanotubes (f-MWCNTs) are increasing. These CNMs can enter the freshwater environment via numerous routes potentially exposing various organisms. The current study assesses the effects of graphene, f-MWCNTs, and their binary mixture on the freshwater algal species Scenedesmus obliquus. The concentration for the individual materials was kept at 1 mg L− 1, while for the mixture, both graphene and f-MWCNTs were taken at 0.5 mg L− 1each. Both the CNMs caused a decrease in cell viability, esterase activity, and photosynthetic efficiency in the cells. The cytotoxic effects were accompanied by an increase in hydroxyl and superoxide radical generation, lipid peroxidation, antioxidant enzyme activity (catalase and superoxide dismutase), and mitochondrial membrane potential. Graphene was more toxic compared to f-MWCNTs. The binary mixture of the pollutants demonstrated a synergistic enhancement of the toxic potential. The oxidative stress generation played a critical role in toxicity responses, as noted by a strong correlation between the physiological parameters and the biomarkers of oxidative stress. The outcomes from this study emphasize the significance of considering the combined effects of various CNMs as part of a thorough evaluation of ecotoxicity in freshwater organisms.

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