Effect of Citrus bergamia juice on human neuroblastoma cells in vitro and in metastatic xenograft models

Navarra, Michele; Ursino, Maria Rita; Ferlazzo, Nadia; Russo, Marina; Schumacher, Udo; Valentiner, Ursula

doi:10.1016/j.fitote.2014.02.009

Cited by 69 publications

(55 citation statements)

References 35 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Cytotoxic effects of Citrus sinensis essential oil were demonstrated by MTT assay in a study with SW480 and HT-29 cells in different times of incubation (Chidambara-Murthy et al, 2012). Similarly, the incubation of LAN-1 and SK-N-SH cells with growing concentrations of Citrus bergamia juice ranging from 0.5 to 10% for 24, 48 and 72h reduced cell proliferation in a concentrationdependent manner (Navarra et al, 2014). Thus, based on our data, our results demonstrated to be safe and gave us support to continue with behavioral tests.…”

Section: Discussionsupporting

confidence: 70%

See 1 more Smart Citation

Anxiolytic-like effect of Citrus limon (L.) Burm f. essential oil inhalation on mice

Viana

Cardoso

Silva

et al. 2016

Rev. bras. plantas med.

View full text Add to dashboard Cite

Experimental in vivo study aimed to characterize the anxiolytic-like effect of the Citrus limon fruit peel's essential oil (CLEO) in animal models of anxiety, besides evaluating the viability J774.A1 cells in vitro through the MTT reduction method at the concentrations of 10 and 100 µg/mL. The anxiolytic behavior was evaluated in Swiss mice (n = 8) using the methodology of Elevated Plus-Maze (EPM) and Open-Field (OF). CLEO was tested by inhalation at the doses of 100, 200, and 400 µL, and as control, animals were subjected to inhalation of the vehicle (saline solution 0.9% + Tween80®) and intraperitoneal administration of diazepam (1.5 mg/kg). In the cell viability assay, it was observed that none of the concentrations showed cytotoxicity. OF test showed significant anxiolytic activity at all tested doses of OECL, compared to the control group, without changing the motor performance of the animals. Corroborating OF data, the EPM test confirmed anxiolytic activity in at least two doses of the tested oil (200 and 400 µL), justified by the number of entries and increase in the percentage of time in the open arms. The data analysis of this study evidenced that inhalation of OECL was able to induce an anxiolytic behavior in mice; however, further studies are required to ensure its safe use by the population. .A1 através do método de redução do MTT nas concentrações de 10 e 100 µg/mL. O comportamento ansiolítico foi avaliado em camundongos Swiss (n = 8), utilizando a metodologia do Labirinto em Cruz Elevado (LCE) e do Campo Aberto (CA). O OECL foi testado por inalação nas doses de 100, 200 e 400 µL, e como controle, os grupos foram submetidos à inalação do veículo (Solução fisiológica 0,9% + Tween80®) e administração intraperitoneal de diazepam (1,5 mg/kg). No ensaio de viabilidade celular, observou-se que nenhuma das concentrações apresentou citotoxicidade. O teste de CA demonstrou atividade ansiolítica significativa em todas as doses testadas do OECL, comparada ao grupo controle, sem alterar a performance motora dos animais. Corroborando os dados do CA, o teste do LCE confirmou a atividade ansiolítica em pelo menos duas das doses do óleo testadas (200 e 400 µL), justificada pelo número de entradas e aumento na percentagem de tempo nos braços abertos. A análise dos dados deste estudo evidenciou que a inalação do OECL foi capaz de induzir um comportamento ansiolítico em camundongos; entretanto, outros estudos são necessários para garantir o seu uso seguro pela população.Palavras-chave: Ansiolítico. Citrus limon. Óleo essencial. Inalação. Camundongos.

show abstract

Section: Discussionsupporting

confidence: 70%

“…Thereby, development of new anxiolytics that do not induce sedative effects and/or inhibit locomotion would be highly useful (Park et al, 2005). Hence, CLEO might serve as an appealing alternative therapeutic target, because the behavior analyzed in the animals is suggestive of anxiolyticlike effect with no motor impairment.…”

Section: Discussionmentioning

confidence: 99%

Anxiolytic-like effect of Citrus limon (L.) Burm f. essential oil inhalation on mice

Viana

Cardoso

Silva

et al. 2016

Rev. bras. plantas med.

View full text Add to dashboard Cite

show abstract

“…Moreover, a clinical research showed that the bergamot-derived polyphenolic fraction, given orally in patients suffering from metabolic syndrome, produces significant reduction of serum cholesterol, triglycerides and glycaemia [28], strengthen the finding obtained in animal model. More recently, we demonstrated that BJ is also able to inhibit important molecular pathways related to cancer-associated aggressive phenotype, thus reducing growth, adhesion and migration in different in vitro [29] and in vivo models [30]. Finally, studies performed on colorectal cancer cell line verified that the antiproliferative effect of BJ is due to its flavonoid fraction which is able to act by multiple mechanisms depending on the concentration [31].…”

Section: Introductionmentioning

confidence: 81%

Flavonoid Fraction of Bergamot Juice Reduces LPS-Induced Inflammatory Response through SIRT1-Mediated NF-κB Inhibition in THP-1 Monocytes

et al. 2014

View full text Add to dashboard Cite

Plant polyphenols exert anti-inflammatory activity through both anti-oxidant effects and modulation of pivotal pro-inflammatory genes. Recently, Citrus bergamia has been studied as a natural source of bioactive molecules with antioxidant activity, but few studies have focused on molecular mechanisms underlying their potential beneficial effects. Several findings have suggested that polyphenols could influence cellular function by acting as activators of SIRT1, a nuclear histone deacetylase, involved in the inhibition of NF-κB signaling. On the basis of these observations we studied the anti-inflammatory effects produced by the flavonoid fraction of the bergamot juice (BJe) in a model of LPS-stimulated THP-1 cell line, focusing on SIRT1-mediated NF-κB inhibition. We demonstrated that BJe inhibited both gene expression and secretion of LPS-induced pro-inflammatory cytokines (IL-6, IL-1β, TNF-α) by a mechanism involving the inhibition of NF-κB activation. In addition, we showed that BJe treatment reversed the LPS-enhanced acetylation of p65 in THP-1 cells. Interestingly, increasing concentrations of Sirtinol were able to suppress the inhibitory effect of BJe via p65 acetylation, underscoring that NF-κB–mediated inflammatory cytokine production may be directly linked to SIRT1 activity. These results suggest that BJe may be useful for the development of alternative pharmacological strategies aimed at reducing the inflammatory process.

show abstract

“…However, bergamot juice, obtained from the endocarp after essential oils extraction, has long time been considered just a secondary and discarded product of the essential oil industry due to its bitter taste. Recently, it gained attention because of its hypolipemic and hypoglycaemic activity (Mollace et al, 2011), as well as its anti-inflammatory (Impellizzeri et al, 2014; Risitano et al, 2014) and anti-cancer properties (Delle Monache et al, 2013; Navarra et al, 2014; Visalli et al, 2014). …”

Section: Introductionmentioning

confidence: 99%

Citrus bergamia essential oil: from basic research to clinical application

et al. 2015

View full text Add to dashboard Cite

Citrus bergamia Risso et Poiteau, also known as “Bergamot,” is a plant belonging to the Rutaceae family, defined as a hybrid of bitter orange and lemon. It is an endemic plant of the Calabria region (Italy). Bergamot fruit is primarily used for the extraction of its essential oil (bergamot essential oil: BEO), employed in perfume, cosmetics, food, and confections. The aim of this review was to collect recent data from the literature on C. bergamia essential oil and, through a critical analysis, focus on safety and the beneficial effects on human health. Clinical studies on the therapeutic applications of BEO exclusively focus on the field of aromatherapy, suggesting that its use can be useful for reducing anxiety and stress.

show abstract

Effect of Citrus bergamia juice on human neuroblastoma cells in vitro and in metastatic xenograft models

Cited by 69 publications

References 35 publications

Anxiolytic-like effect of Citrus limon (L.) Burm f. essential oil inhalation on mice

Anxiolytic-like effect of Citrus limon (L.) Burm f. essential oil inhalation on mice

Flavonoid Fraction of Bergamot Juice Reduces LPS-Induced Inflammatory Response through SIRT1-Mediated NF-κB Inhibition in THP-1 Monocytes

Citrus bergamia essential oil: from basic research to clinical application

Contact Info

Product

Resources

About