Effect of Cleaners and Sanitizers on Listeria monocytogenes Attached to Product Contact Surfaces

Krysinski, E. P.; Brown, L. Jackson; Marchisello, T. J.

doi:10.4315/0362-028x-55.4.246

Cited by 208 publications

(109 citation statements)

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“…There have been reports of results similar to our experiment (13). In this research, the hygienic conditions of 86 different surfaces of a line of fluid milk in 4 dairy plants were evaluated by the ATP bioluminescence technique and mesophilic aerobic counts.…”

Section: Resultssupporting

confidence: 70%

“…In our experiment, the influence of chemical agent residues, basically sodium hydroxide applied to remove organic substances, such as proteins and fats from surfaces was not evaluated, but some researches have demonstrated that the RLU measurement can be affected by chemical cleaning agents and commercial sanitizers (9,10,13). An ATP extractant effect is attributed to detergents and sanitizers solutions used in cleaning and sanitizing procedures.…”

Section: Resultsmentioning

confidence: 99%

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ATP-bioluminescence assay as an alternative for hygiene-monitoring procedures of stainless steel milk contact surfaces

Costa

Andrade

Brandão

et al. 2006

Braz. J. Microbiol.

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ATP-bioluminescence assay was used to evaluate the cleaning and sanitizing procedures of stainless steel milk contact surfaces. Surfaces of a raw milk transportation tank, a raw milk cooling storage tank, an equalization pasteurizing tank, a pasteurized milk storage tank and a pasteurized milk packaging tank, as well as a milk centrifuge were evaluated. The Relative Light Unit (RLU) using a commercial luminometer and mesophilic aerobic counts (CFU.cm -2 ) on surfaces were determined. There were no significant differences (p>0.05) in RLU on the assessed surfaces before and after the cleaning and sanitizing procedures. Also, there were no significant differences (p>0.05) in CFU.cm -2 on the surfaces before the cleaning and sanitizing procedures, whereas different bacterial numbers were found on surfaces after the hygiene procedures. The surfaces of the milk centrifuge, raw milk cooling tank and milk packaging tank showed the largest mesophilic aerobic numbers after the hygiene, reaching 2.21, 2.18, and 1.21 log10, respectively. The results demonstrated that there is no agreement among the techniques for classification of hygienic conditions of the evaluated surfaces, showing a non-direct relation between RLU and CFU.cm . The ATP-bioluminescence method showed that 100% of the surfaces were under inadequate hygiene conditions, while the plate count method detected only 50%, based on the APHA's recommendation, and 33%, based on the WHO's recommendation. High variations in RLU measurements were observed, suggesting the need for more than only one surface analysis. The ATPbioluminescence is not a direct replacement for microbiological testing, but provides complementary information and should be used as part of an integrated strategy.

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Section: Resultssupporting

confidence: 70%

Section: Resultsmentioning

confidence: 99%

ATP-bioluminescence assay as an alternative for hygiene-monitoring procedures of stainless steel milk contact surfaces

Costa

Andrade

Brandão

et al. 2006

Braz. J. Microbiol.

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“…Our results contrast with those of other authors (KRYSINSKI; BROWN; MARCHISELLO, 1992;RONNER;WONG, 1993;ABRISHAMI et al, 1994;BOURION;CERF, 1996;LINDSAY;VON HOLY, 1999;BREMER;MONK;BUTTLER, 2002), who reported that resistance of bacteria to sanitizers was influenced by the type of surface. These discrepancies could be explained, at least in part, by the fact that they challenged different sanitizers, bacterial species, or attachment surfaces.…”

Section: Effect Of Heatcontrasting

confidence: 99%

Evaluation of the efficacy of commercial sanitizers against adhered and planktonic cells of Listeria monocytogenes and Salmonella spp.

Carballo

Araujo

2012

Food Sci. Technol

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Evaluation of the efficacy of commercial sanitizers against adhered and planktonic cells of Listeria monocytogenes and Salmonella spp. Avaliação da eficiência de desinfetantes comerciais contra células aderidas e planctônicas de Listeria monocytogenes e Salmonella spp.Julia CARBALLO 1 *, Ana-Belén ARAÚJO 1 IntroductionCross contamination of food products due to contact with food processing surfaces might be a major problem in the food industry (KUSUMANINGRUM et al., 2003a). The persistence of bacteria on surfaces has been suggested to relate to their attachment to food contact materials (GRAVESEN; LEKKAS; KNØCHEL, 2005;VALERIANO et al., 2012). Pathogenic bacteria may remain on equipment surfaces increasing the risks of transmission of diseases (SILVA et al., 2008;BELESSI et al., 2011).Salmonella spp. and Listeria monocytogenes are examples of pathogenic bacteria implicated in outbreaks associated with the ingestion of contaminated food (LO FO WONG et al., 2002;GANDHI;CHIKINDAS, 2007). It has been proved that Salmonella Typhimurium can survive up to four weeks on dry surfaces in high-enough populations to be transferred to foods immediately on contact (DAWSON et al., 2007). It has also been found that L. monocytogenes can be transferred from foods to surfaces (RODRIGUEZ; AUTIO; and reciprocally . Indeed, it has been shown that these bacteria are able to attach to food contact surfaces (SINDE; CARBALLO, 2000;MAI et al., 2006;OLIVEIRA et al., 2006).Research in this area has indicated that adherent microorganisms may be much more resistant to sanitizing compounds than planktonic cells (JOSEPH et al., 2001;KUSUMANINGRUM et al., 2003b; ALI et al., 2006).The efficacy of the cleaning and disinfection operations of surfaces in the food industry depends on the design and type of surfaces, as well as on the procedures and products used. The outcome of these operations is limited by the ability of bacteria ResumoNeste estudo, foi avaliada a atividade antimicrobiana de dois desinfetantes comerciais, individualmente e combinados com calor, contra três estirpes de Salmonella e três de Listeria monocytogenes. A eficácia dos desinfetantes foi investigada contra bactérias em suspensão e contra bactérias aderidas a três superfícies de contacto comuns na indústria de alimentos (aço inoxidável, politetrafluoretileno e borracha). Os ensaios foram realizados com base nas concentrações de desinfetantes recomendadas pelos fabricantes e em concentração dupla e quádrupla. As concentrações recomendadas não foram efetivas contra as bactérias, especialmente quando estas estavam aderidas às superfícies. Foi necessário o dobro ou o quádruplo da concentração recomendada de desinfetante para eliminar as bactérias em suspensão. Essas mesmas concentrações não foram suficientes para eliminar as bactérias aderidas. De forma a erradicá-las da superfície, foi necessário um tratamento com a concentração recomendada em combinação com calor. Os resultados indicaram que essas estirpes patogênicas podem sobreviver a tratamentos de desinfecção habitualmente...

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“…To this respect, there are several reports on the in vitro capacity of these bacteria to adhere to stainless steel laminae and/or synthetic polymers [14][15][16][17][18].…”

Section: Open Access Research Articlementioning

confidence: 99%

“…To this respect, there are several reports on the in vitro capacity of these bacteria to adhere to stainless steel laminae and/or synthetic polymers [14][15][16][17][18].Bacterial adhesion to a surface is a two-phases process: in the first phase, planktonic bacteria are moved to a surface by the effect of physical forces such as Brownian motion, van der Waals attraction forces, gravitational forces, the effect of surface electrostatic charge and hydrophobic interactions; in the second phase of adhesion, molecular irreversible reactions between bacterial surface structures and substratum surfaces become predominant [19,20]. Adhesion is an essential stage for the formation and subsequent growth of biofilms [19,21,22].…”

mentioning

confidence: 99%

Adhesion Capacity of Bacillus thuringiensis Spores and its Relation with Biofilm Formation

León¹

2015

SOJMID

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Open Access Research Articlehaving an attenuated immuneresponse [7].It is known that Bt spores and those of other Bacillus species, presenting exosporium, have a higher hydrophobicity, conferring them a higher adhesive potential to diverse materials [8,9], such as those used in industrial food processing [1,[10][11][12][13]. To this respect, there are several reports on the in vitro capacity of these bacteria to adhere to stainless steel laminae and/or synthetic polymers [14][15][16][17][18].Bacterial adhesion to a surface is a two-phases process: in the first phase, planktonic bacteria are moved to a surface by the effect of physical forces such as Brownian motion, van der Waals attraction forces, gravitational forces, the effect of surface electrostatic charge and hydrophobic interactions; in the second phase of adhesion, molecular irreversible reactions between bacterial surface structures and substratum surfaces become predominant [19,20]. Adhesion is an essential stage for the formation and subsequent growth of biofilms [19,21,22].Biofilms are defined as microbial communities embedded in a polymer layer or matrix adhered to an inert surface or substrate, or to living cells or tissues [23,24]. Several members of the Bacillus genus are capable of forming biofilms in vitro at variable magnitudes dependent on the incubation time [6,25]. According to Auger et al. [25], less than 50% of the B. cereus and Bt strains showed capacity to form biofilm in their assays. Formation of biofilms has been related also to the resistance of these species to diverse antibiotics and disinfectants [26,27] found that the latent spores of B. cereus were more efficient in starting and forming biofilms as compared to bacterial cells in vegetative or sessile state. These same authors evidenced that the production of biofilm in cultures with spores depends on the growth curves, which does not occur in cultures in suspension, and could be related to the resistance of the biofilm to adverse factors.Despite the existence of important collections of Bt isolated from different regions of Mexico, few studies have focused on the possible associations and distribution of features that could contribute to the analysis and a better understanding of the relations at the level of species and subspecies of the very AbstractSpores of Bacillus thuringiensis (Bt), used as bioinsecticide, represent a potential contamination risk of the equipment used for food processing and could also induce potential gastrointestinal symptoms in special cases of susceptible individuals. The objective of this work was to explore the in vitro adhesion capacity of Bt spores and its relation to the ability to form biofilms. The adhering capacity of spores of 65 Bt strains was determined in stainless steel tubes, as well as their ability to form biofilms on polystyrene microplates. We analyzed the relation of these two features. All strains showed a variable adhering capacity to stainless steel, ranging from 0.04% to 1.21% of the initial spore load of ~1.7×10 7 CFU/ mL. Th...

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Effect of Cleaners and Sanitizers on Listeria monocytogenes Attached to Product Contact Surfaces

Cited by 208 publications

References 0 publications

ATP-bioluminescence assay as an alternative for hygiene-monitoring procedures of stainless steel milk contact surfaces

ATP-bioluminescence assay as an alternative for hygiene-monitoring procedures of stainless steel milk contact surfaces

Evaluation of the efficacy of commercial sanitizers against adhered and planktonic cells of Listeria monocytogenes and Salmonella spp.

Adhesion Capacity of Bacillus thuringiensis Spores and its Relation with Biofilm Formation

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