Three groups of 6 healthy mature Yankasa rams aged 2.0 to 2.6 years weighing between 30.50 and 30.60kg were used to determine the effect of Pergonal ® on daily sperm output, gonadal and extragonadal sperm reserves. The rams were assigned to either 49.50 i.u (T2), 99.00 i.u (T3) or 148.50 i.u (T4) Pergonal ® injections (Ferring Labs, USA) each divided into 3 doses and administered for 3 consecutive days according to the manufacturer's prescription. Another group of 6 rams was given normal saline (1ml) during the same period to serve as control (T1). All treatments were given to study the effect of the drug on daily sperm output, gonadal and extragonadal sperm reserves. All the treatments were given by intra-muscular injection. The results showed significant differences (P>0.05) among the treatment groups in daily sperm output, (x10 9) daily sperm output per gram testis (x10 9), gonadal sperm reserve (x10 9), caput sperm reserve (x10 8), corpus sperm reserve (x10 8), cauda sperm reserve (x10 8) and vas deferens sperm reserve (x10 8). High correlations were observed between testis weight, caput weight and daily sperm output, gonadal sperm reserve, daily sperm output per gram testis, between caput weight, corpus weight, cauda weight and cauda sperm reserve. The results of this study suggest that the daily sperm output, daily sperm output per gram testis, gonadal, caput, corpus, cauda and vas deferens sperm reserves would be improved when 99.00 i.u of Pergonal ® is used for induction of spermatogenesis in Yankasa rams.