Effect of co-administration of extracts of Vernonia amygdalina and Azadirachta indica on lipid profile and oxidative stress in hepatocytes of normal and diabetic rats

Abstract

Effect of combined administration of extracts of Vernonia amydalina (VA) and Azadirachta indica (AI) on oxidative stress indices and serum lipid profile of normal and diabetic rats was evaluated. Result indicated a significant decrease (p<0.05) in catalase (CAT) activity, but non-significant decreases (p>0.05) in superoxide dismutase (SOD) and glutathione peroxidase (GPx) activities, and in the extent of lipid peroxidation non-significant increase (p>0.05) in malondialdehyde (MDA) concentration of untreated diabetic rats relative to the normal control. Treatment with combined extracts of VA and AI caused significant elevation in GPx, SOD and CAT activities, and reduction in MDA concentration in hepatocytes, hence antioxidant activity. This antioxidant potential was also demonstrated in normal rats which received the treatment. The combined extracts also caused mild increase and decrease in serum high density lipoprotein (HDL) cholesterol and triacylglycerol concentrations, but a respective significant (p<0.05) decrease and increase in serum total cholesterol (TC) and anti-atherogenic index (HDL/TC) when compared to diabetic control. Co-administration of extracts of VA and AI may positively modulate the antioxidant defense system and prevent atherosclerosis in diabetes.

Protective Effects of Parkia biglobosa Protein Isolate on Streptozotocin-Induced Hepatic Damage and Oxidative Stress in Diabetic Male Rats

“…It is believed that in STZ-diabetic conditions, hyperglycaemia produces ROS that exceeds endogenous antioxidant capacity; these ROS may react with polyunsaturated fatty acids of cell membranes thereby causing lipid peroxidation and membrane damage [ 35 , 36 ]. The result of the present study revealed that TBARS level in the liver of STZ-induced diabetic rats was markedly elevated when compared with the control.…”

“…Added to this, SOD and CAT play an essential role in attenuating cellular stress by maintaining physiological levels of oxygen and hydrogen peroxide. SOD scavenges the superoxide radical by converting it to hydrogen peroxide and molecular oxygen, while CAT brings about the reduction of hydrogen peroxides and protects higher tissues from the highly reactive hydroxyl radicals [ 35 , 41 ]. Interestingly, administration of PBPi to STZ-induced diabetic rats considerably revert the hepatic antioxidant status in a dose-dependent manner near normal when compared with the control group.…”

Anti-hepatotoxic and antioxidant influence of Ipomoea carnea against anti-tubercular drugs induced acute hepatopathy in experimental rodents

“…Pyrazinamide, in combination with isoniazid and rifampicin is also, associated with an increased incidence of hepatotoxicity [30] . In addition to these mechanisms, oxidative stress induced hepatic injury is one of the important mechanisms in hepatotoxicity produced by antitubercular drugs [31] .…”