Effect of co-inoculation of methylotrophic Methylobacterium oryzae with Azospirillum brasilense and Burkholderia pyrrocinia on the growth and nutrient uptake of tomato, red pepper and rice

Abstract

The present greenhouse study was undertaken to evaluate the effects of co-inoculating methylotrophic Methylobacterium oryzae CBMB20 along with nitrogen-fixing Azospirillum brasilense CW903 or a phosphate solubilizing bacterium Burkholderia pyrrocinia CBPB-HOD on the growth and nutrient uptake of tomato, red pepper and rice. Seed inoculation and soil/foliar application of the bacterial strains alone or under dual inoculation increased the plant growth in terms of shoot or root length and increased the nutrient uptake in the plants studied compared to uninoculated control plants. Co-inoculation of M. oryzae CBMB20 with A. brasilense CW903 or B. pyrrocinia CBPB-HOD improved the N and P concentration of plants, while the results varied among the plant species tested. Also, co-inoculation of the bacterial strains increased the activity of nitrogenase, urease and phosphatase enzymes in soil when compared to uninoculated control or individual inoculations. Though the inoculation effects were analyzed at an early stage of plant growth, the results conclusively suggest that M. oryzae being compatible with other microorganisms in the rhizosphere can potentially be used as individual inoculant or co-inoculated with other plant growth promoting bacteria to increase the production in sustainable agricultural systems.

Effect of Azospirillum brasilense and Methylobacterium oryzae Inoculation on Growth of Red Pepper (Capsicum annuum L.)

“…Although the positive responses of crop plants to inoculation of A. brasilense CW903 and M. oryzae CBMB20 were clearly demonstrated under controlled conditions (Kim et al, 2011;Madhaiyan et al, 2010;Ryu et al, 2006), in this study conducted under field condition, inoculations of A. brasilense CW903 and M. oryzae CBMB20 were not effective on promotion of the growth and fruit yield of red pepper plant. Bashan et al (2004) suggested that the good positive plant growth-promoting effects obtained under pot experiments cannot always be dependably reproduced under field conditions.…”

“…These results indicate that the positive plant growthpromoting potential of the inoculants expressed in pot experiments cannot always be reproduced under field conditions. As demonstrated in numerous worldwide trials, we have already found positive effects of A. brasilense CW903 and M. oryzae CBMB20 inoculation on plant growth in the previous laboratory and pot experiments with wheat, rice, red pepper, tomato, canola, and groundnut (Madhaiyan et al, 2004;Madhaiyan et al, 2006a;Madhaiyan et al, 2006b;Madhaiyan et al, 2007;Madhaiyan et al, 2010;Ryu et al, 2006). Plant growth promoting effect of A. brasilense could be attributed to several mechanisms including enhancement of root development, production of growth regulators and nitrogen fixation (Bashan and Holguin, 1997;Kim et al, 2005).…”

“…Various bacterial species have been successfully used in inoculation trials in pot experiments under greenhouse condition (Bashan and Holguin, 1997;Masheshawari, 2011). However, commercial application of plant growth promoting microbes on a large scale is still very limited, and the main obstacle is the unpredictability and inconsistency of field results (Bashan and Holguin, 1997 mg kg Recently we isolated bacterial species of Methylobacterium and Azospirillum from rice and taro, respectively, and their plant growth promoting abilities were confirmed in laboratory and greenhouse experiments (Chauhan et al, 2010;Kim et al, 2005;Madhaiyan et al, 2004Madhaiyan et al, , 2007Madhaiyan et al, , 2010Ryu et al, 2006). Methylobacterium spp.…”

“…While appropriate application of chemical fertilizers is essential in modern farming systems, deleterious environmental impacts of fertilization should be minimized. Using plant growth promoting microbes, possessing the versatile plant-beneficial traits, fertilizer application can be reduced (Adesemoye et al, 2008;Ahemad and Khan, 2011;Dobbelaere et al, 2002;Kloepper et al, 2004;Madhaiyan et al, 2004Madhaiyan et al, , 2009Madhaiyan et al, , 2010Vessey, 2003;Weller, 2007). Various bacterial species have been successfully used in inoculation trials in pot experiments under greenhouse condition (Bashan and Holguin, 1997;Masheshawari, 2011).…”

Advances in plant growth-promoting bacterial inoculant technology: formulations and practical perspectives (1998–2013)

“…Additionally, inoculants must overcome two major problems inherent to living microorganisms: (1) loss of viability during short storage in the grower's warehouse, and (2) long shelf life and stability of the product over a range of −5 to 30°C, within growers' storage conditions. Anith et al 2004;Bacilio et al 2003Bacilio et al , 2004Bashan et al 2006;Boruah and Kumar 2002;Cakmakci et al 2001Cakmakci et al , 2006Carrillo-Garcia et al 2000;Dominguez-Nuñez et al 2012;Egamberdiyeva 2007;Esitken et al 2003;Fürnkranz et al 2012;Guetsky et al 2002a, b;Guiñazú et al 2010;Hossain and Mårtensson 2008;Jetiyanon et al 2003;Khan et al 2003;Kurek and Jaroszuk-Ściseł 2002;Landa et al 2001;Lopez et al 2013;Madhaiyan et al 2010;Mehnaz et al 2010;Odee et al 2002;Ozturk et al 2003;Puente et al 2004aPuente et al , b, 2009aRajapaksha et al 2011;Singh et al 2011;Sigler et al 2001;Sudhakar et al 2000;Yan et al 2003;Yu et al 2011;Zafar et al 2012 Liquid (culture media or water) Clayton et al 2004a, b;Hamaoui et al 2001;Hungria et al 2010; Bashan et al 2002a, b;deBashan et al 2004;…”